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Dietary effects of diacylglycerol rich mustard oil on lipid profile of normocholesterolemic and hypercholesterolemic rats

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Abstract

Several recent studies have established that diacylglycerol (DAG) rich oils significantly reduce the body weight. The present study was conducted to evaluate the dietary effects of DAG- rich mustard oil on normal and hypercholesterolemic rats. DAG- rich mustard oil (45.5% DAG) was prepared in the laboratory by enzymatic glycerolysis process. For the feeding experiment, 32 rats were taken and divided into four groups (average body weight 130 g) and body weight gain, food efficiency ratio, lipid profile of plasma, liver, mesentery and erythrocytes membrane (EM), HMG Co-A reductase activity and plasma leptin content were measured and compared with the normal TAG-rich diet. The dietary DAG rich mustard oil significantly decreased body weight and FER compared to TAG rich mustard oil both in normal and hypercholesterolemic rats. The total cholesterol content was decreased with significant increase in HDL- cholesterol by feeding DAG rich diet. Total lipid and TAG content of both liver and mesentery were significantly decreased in DAG diet group compared to control group. Liver HMG CoA: mevalonate ratio was also found to be significantly decreased in the DAG group. Blood leptin level significantly reduced with DAG rich diet compared to the TAG rich dietary groups.

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References

  • Allain CC, Poon LS, Chan CS, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20(4):470–475

    CAS  Google Scholar 

  • Bauer JE, Nagaoka D, Porterpan B, Bigley K, Umeda T, Otsuji K (2006) Postprandial lipolytic activities, lipids, and carbohydrate metabolism are altered in dogs fed diacylglycerol meals containing high- and low-glycemic-index starches. J Nutr 136(7 Suppl):1955S–1957S. doi:136/7/1955S

    CAS  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917

    Article  CAS  Google Scholar 

  • Bucolo G, David H (1973) Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem 19(5):476–482

    CAS  Google Scholar 

  • Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E, Novak S, Collins C, Welch CB, Lusis AJ, Erickson SK, Farese RV Jr (1998) Identification of a gene encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc Natl Acad Sci USA 95(22):13018–13023

    Article  CAS  Google Scholar 

  • Chen PS, Toribara TY, Warner H (1956) Microdetermination of phosphorous. Anal Chem 28:1756–1758

    Article  CAS  Google Scholar 

  • Dhara R, Ghosh M (2009) Lipase-catalyzed synthesis of diacylglycerol rich oil from mustard oil (Brassica juncea). In: Socio-economic development: challenges before women scientist, technologists & engineers. Excel India Publishers, New Delhi, pp 173–178

  • Fernandes MLM, Krieger N, Baron AM, Zamora PP, Ramos LP, Mitchell DA (2004) Hydrolysis and synthesis reactions catalysed by Thermomyces lanuginosa lipase. J Mol Catal B 30:43–49

    Article  CAS  Google Scholar 

  • Frayn KN (2002) Adipose tissue as a buffer for daily lipid flux. Diabetologia 45(9):1201–1210. doi:10.1007/s00125-002-0873-y

    Article  CAS  Google Scholar 

  • Hibi M, Takase H, Meguro S, Tokimitsu I (2009) The effects of diacylglycerol oil on fat oxidation and energy expenditure in humans and animals. Biofactors 35(2):175–177. doi:10.1002/biof.25

    Article  CAS  Google Scholar 

  • Hibi M, Sugiura Y, Yokoyama R, Takase H, Shiiba D, Meguro S, Katashima M, Shimizu A, Tokimitsu I (2011) The short-term effect of diacylglycerol oil consumption on total and dietary fat utilization in overweight women. Obesity (Silver Spring) 19(3):536–540. doi:oby201017310.1038/oby.2010.173

    Article  CAS  Google Scholar 

  • Ikeda I, Yanagita T (2004) Fate of hydrolysis product originating from diacylglycerol and triacylglycerol in the intestine. In: Diacylglecerol Oil. AOCS press, Champaign, Illinois, pp 64–69

  • John C (1976) Degumming, refining, bleaching, and deodorization theory. J Am Oil Chem Soc 6:53–56

    Google Scholar 

  • Jones JH, Foster CA (1942) A salt mixture for use with basal diet either low or high in phosphorus. J Nutr 24:245–256

    CAS  Google Scholar 

  • Kamphuis MM, Mela DJ, Westerterp-Plantenga MS (2003) Diacylglycerols affect substrate oxidation and appetite in humans. Am J Clin Nutr 77(5):1133–1139

    CAS  Google Scholar 

  • Kannel WB, Cupples LA, Ramaswami R, Stokes J 3rd, Kreger BE, Higgins M (1991) Regional obesity and risk of cardiovascular disease; the Framingham Study. J Clin Epidemiol 44(2):183–190

    Article  CAS  Google Scholar 

  • Kimuru S, Tsuchuya H, Inage H (2003) Effect of dietary diacylglycerol on the energy metabolism. In: AOCS Annual Meeting, Kansas City, May 4–7 2003

  • Kokie S, Hosaya N, Yasumasu T (2001) Oil composition and use thereof. European Patent EP1135991 (in English)

  • Lehner R, Kuksis A (1993) Triacylglycerol synthesis by an sn-1,2(2,3)-diacylglycerol transacylase from rat intestinal microsomes. J Biol Chem 268(12):8781–8786

    CAS  Google Scholar 

  • Litchfield C (1972) Analysis of triglycerides. Academic, New York

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275

    CAS  Google Scholar 

  • Macala LJ, Yu RK, Ando S (1983) Analysis of brain lipids by high performance thin-layer chromatography and densitometry. J Lipid Res 24(9):1243–1250

    CAS  Google Scholar 

  • Maki KC, Davidson MH, Tsushima R, Matsuo N, Tokimitsu I, Umporowicz DM, Dicklin MR, Foster GS, Ingram KA, Anderson BD, Frost SD, Bell M (2002) Consumption of diacylglycerol oil as part of a reduced-energy diet enhances loss of body weight and fat in comparison with consumption of a triacylglycerol control oil. Am J Clin Nutr 76(6):1230–1236

    CAS  Google Scholar 

  • Masui K, Katsuragi Y, Toi T (2001) Fat or oil composition. Patent International Application no.WO01011787 (in English)

  • Matsuzawa Y, Nakamura T, Shimomura I, Kotani K (1995) Visceral fat accumulation and cardiovascular disease. Obes Res 3(Suppl 5):645S–647S

    Google Scholar 

  • Mori Y, Nakagiri H, Kondo H, Murase T, Tokimitsu I, Tajima N (2005) Dietary diacylglycerol reduces postprandial hyperlipidemia and ameliorates glucose intolerance in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Nutrition 21(9):933–939. doi:10.1016/j.nut.2005.01.009

    Article  CAS  Google Scholar 

  • Murata M, Ide T, Hara K (1997) Reciprocal responses to dietary diacylglycerol of hepatic enzymes of fatty acid synthesis and oxidation in the rat. Br J Nutr 77(1):107–121. doi:S0007114597000159

    Article  CAS  Google Scholar 

  • Murase T, Mizuno T, Omachi T, Onizawa K, Komine Y, Kondo H, Hase T, Tokimitsu I (2001) Dietary diacylglycerol suppresses high fat and high sucrose diet-induced body fat accumulation in C57BL/6 J mice. J Lipid Res 42(3):372–378

    CAS  Google Scholar 

  • Murase T, Aoki M, Wakisaka T, Hase T, Tokimitsu I (2002) Anti-obesity effect of dietary diacylglycerol in C57BL/6 J mice: dietary diacylglycerol stimulates intestinal lipid metabolism. J Lipid Res 43(8):1312–1319

    CAS  Google Scholar 

  • Nagao T, Watanabe H, Goto N, Onizawa K, Taguchi H, Matsuo N, Yasukawa T, Tsushima R, Shimasaki H, Itakura H (2000) Dietary diacylglycerol suppresses accumulation of body fat compared to triacylglycerol in men in a double-blind controlled trial. J Nutr 130(4):792–797

    CAS  Google Scholar 

  • Osaki N, Meguro S, Yajima N, Matsuo N, Tokimitsu I, Shimasaki H (2005) Metabolites of dietary triacylglycerol and diacylglycerol during the digestion process in rats. Lipids 40(3):281–286

    Article  CAS  Google Scholar 

  • Pi-Sunyer FX (1991) Health implication of obesity. Am J Clin Nutr 53:1595–1603

    Google Scholar 

  • Rao AV, Ramakrishnan S (1975) Indirect assessment of hydroxymethylglutaryl-CoA reductase (NADPH) activity in liver tissue. Clin Chem 21(10):1523–1525

    CAS  Google Scholar 

  • Rose HG, Oklander M (1965) Improved procedure for the extraction of lipids from human erythrocytes. J Lipid Res 6:428–431

    CAS  Google Scholar 

  • Rudkowska I, Roynette CE, Demonty I, Vanstone CA, Jew S, Jones PJ (2005) Diacylglycerol: efficacy and mechanism of action of an anti-obesity agent. Obes Res 13(11):1864–1876. doi:10.1038/oby.2005.229

    Article  CAS  Google Scholar 

  • Saito S, Yamaguchi T, Shoji K, Hibi M, Sugita T, Takase H (2010) Effect of low concentration of diacylglycerol on mildly postprandial hypertriglyceridemia. Atherosclerosis 213(2):539–544. doi:10.1016/j.atherosclerosis.2010.07.062

    Article  CAS  Google Scholar 

  • Scheffe H (1961) The analysis of variance. In, vol Sec. 2.2; Sec. 3.6. John Wiley & Sons, New York, pp 27–28; 73–74

  • Tada N, Watanabe H, Matsuo N, Tokimitsu I, Okazaki M (2001) Dynamics of postprandial remnant-like lipoprotein particles in serum after loading of diacylglycerols. Clin Chim Acta 311(2):109–117. doi:S0009898101005836

    Article  CAS  Google Scholar 

  • Taguchi H, Watanabe H, Onizawa K, Nagao T, Gotoh N, Yasukawa T, Tsushima R, Shimasaki H, Itakura H (2000) Double-blind controlled study on the effects of dietary diacylglycerol on postprandial serum and chylomicron triacylglycerol responses in healthy humans. J Am Coll Nutr 19(6):789–796

    CAS  Google Scholar 

  • Taguchi H, Nagao T, Watanabe H, Onizawa K, Matsuo N, Tokimitsu I, Itakura H (2001) Energy value and digestibility of dietary oil containing mainly 1,3-diacylglycerol are similar to those of triacylglycerol. Lipids 36(4):379–382

    Article  CAS  Google Scholar 

  • Tomonobu K, Hase T, Tokimitsu I (2006) Dietary diacylglycerol in a typical meal suppresses postprandial increases in serum lipid levels compared with dietary triacylglycerol. Nutrition 22(2):128–135. doi:10.1016/j.nut.2005.04.016

    Article  CAS  Google Scholar 

  • Umeda T, Bauer JE, Otsuji K (2006) Weight loss effect of dietary diacylglycerol in obese dogs. J Anim Physiol Anim Nutr (Berl) 90(5–6):208–215. doi:10.1111/j.1439-0396.2005.00592.x

    Article  CAS  Google Scholar 

  • Wang W, Xu T, Li X, Zhu Q, Cheng A, Du F, Li D (2010) Effect of diacylglycerol supplementation on fasting serum triacylglycerol concentration: a meta-analysis. Lipids 45(12):1139–1146. doi:10.1007/s11745-010-3478-0

    Article  CAS  Google Scholar 

  • Warnick GR, Nguyen T, Albers AA (1985) Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol. Clin Chem 31(2):217–222

    CAS  Google Scholar 

  • Watanabe H, Tokimitsu I (2004) Diacylglecerol oil. In. AOCS press, pp 30–45

  • Watanabe H, Onizawa K, Taguchi H (1997) Nutritional characterization of diacylglycerols in rats. J Oleo Sci 46:301–307

    CAS  Google Scholar 

  • Wills ED (ed) (1987) Evaluation of lipid peroxidation in lipids and biological membranes in biological toxicology. A practical approach. IRL Press, Oxford

    Google Scholar 

  • Yamamoto K, Asakawa H, Tokunaga K, Watanabe H, Matsuo N, Tokimitsu I, Yagi N (2001) Long-term ingestion of dietary diacylglycerol lowers serum triacylglycerol in type II diabetic patients with hypertriglyceridemia. J Nutr 131(12):3204–3207

    CAS  Google Scholar 

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Acknowledgement

The work was funded by University Grants Commission, Govt. of India. Help and suggestions obtained from Prof. D.K. Bhattacharyya and Dr. Santinath Ghosh are gratefully acknowledged.

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Authors and Affiliations

  1. Dept. of Chemical Technology, University of Calcutta, Kolkata, India

    Rupali Dhara & Mahua Ghosh

  2. Dept. of Home Science, University of Calcutta, Kolkata, India

    Pubali Dhar

  3. Oil Technology Division, Department of Chemical Technology, University of Calcutta, 92, Acharyya Prafulla Chandra Road, Kolkata, 700009, India

    Mahua Ghosh

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  1. Rupali Dhara
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Correspondence to Mahua Ghosh.

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Dhara, R., Dhar, P. & Ghosh, M. Dietary effects of diacylglycerol rich mustard oil on lipid profile of normocholesterolemic and hypercholesterolemic rats. J Food Sci Technol 50, 678–686 (2013). https://doi.org/10.1007/s13197-011-0388-y

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  • DOI: https://doi.org/10.1007/s13197-011-0388-y

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