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Effect of germination periods and hydrothermal treatments on in vitro protein and starch digestibility of germinated legumes

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Abstract

Germination of legumes followed by hydrothermal treatments is an effective mean of improving nutritive value of legumes. The protein content of mungbean, chickpea and cowpea increased by 9–11, 11–16 and 8–11% after germination. A significant (p ≤ 0.05) decrease in protein content was observed on pressure cooking and microwaving in all three legumes. The carbohydrates decreased by 1 to 3% during soaking and 2 to 6% during germination. A significant (p ≤ 0.05) improvement in in vitro protein digestibility (IVPD) was observed after soaking as well as after three germination periods. Germination resulted in an increase in IVPD from 15 to 25% in mungbean, 6 to 17% in chickpea and 6 to 17% in cowpea. A significant (p ≤ 0.05) increase in IVPD was observed when raw sprouts of three legumes were subjected to pressure cooking and microwaving. In vitro starch digestibility (IVSD) increased significantly (p ≤ 0.05) after germination, the percent increase being 8 to 12% in mungbean, 9 to 11% in chickpea and 10 to 13% in cowpea. The duration of germination had significant (p ≤ 0.05) effect on IVSD. A significant (p ≤ 0.05) improvement in IVSD was observed when legume sprouts were subjected to pressure cooking and microwave cooking.

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References

  • Akeson WR, Stachmann MA (1964) A pepsin pancreatin digest index of protein quality evaluation. J Nutr 83:257–261

    CAS  Google Scholar 

  • Akinyele IO, Akinlosotu A (1991) Effect of soaking, dehulling and fermentation on the oligosaccharides and nutrient content of cowpeas (Vigna unguiculata). Food Chem 41:43–53

    Article  CAS  Google Scholar 

  • AOAC (1990) Official methods of analysis (15th edn) Association of Official Analytical Chemists. Washington DC, USA

  • Barakoti L (2004) Development of recipes to enhance the bioavailability of iron from mungbean (Vigna radiata). M.Sc. Thesis, Punjab Agricultural University, Ludhiana

  • Colmenares De Ruiz AS, Bressani R (1990) Effect of germination on the chemical composition and nutritive value of amaranth grain. Cereal Chem 67:519–522

    CAS  Google Scholar 

  • Dhaliwal YS, Aggarwal RAK (1999) Composition of fat in soybeans as affected by duration of germination and drying temperature. J Food Sci Technol 36:266–267

    Google Scholar 

  • Dogra J, Dhaliwal YS, Kalia M (2001) Effect of soaking, germination, heating and roasting on the chemical composition and nutritional quality of soyabean and its utilization in various Indian leavened products. J Food Sci Technol 38:453–457

    CAS  Google Scholar 

  • Jimenez MJ, Elias LG, Bressani R, Navarrete DA, Gomez-Brenes R, Molina MR (1985) Biochemical and nutritional studies of germinated soybean seeds. Arch Latinoam Nutr 35:480–490

    CAS  Google Scholar 

  • Kelkar K, Shastri P, Rao BY (1996) Effect of processing on in vitro carbohydrate digestibility of cereals and legumes. J Food Sci Technol 33:493–497

    Google Scholar 

  • Khadar V (1983) Nutritional studies on fermented, germinated and baked soybean (Glycine max) preparations. J Plant Foods 5:31–37

    Google Scholar 

  • Khalil JK, Sawaya WN, Al-Mohammad HM (1986) Effect of experimental cooking on the yield and proximate composition of three selected legumes. J Food Sci 41:233–235

    Article  Google Scholar 

  • Khatoon N, Prakash J (2005) Cooking quality and sensory profile of microwave and pressure cooked legumes. Indian J Nutr Diet 42:13–21

    Google Scholar 

  • King RD, Puswastein P (1987) Effect of germination on proximate composition and nutritional quality of winged bean seed. J Sci Food Agric 52:104–106

    Google Scholar 

  • Malleshi NG, Klopfenstein CF (1996) Proximate composition, amino acid and vitamin contents of malted chickpea, mungbean and their seed coats. J Food Sci Technol 33:479–482

    CAS  Google Scholar 

  • Mehta P, Bedi B (1993) In vitro protein and starch digestibility in selected germinated legume flours. Indian J Nutr Diet 30:149–153

    Google Scholar 

  • Opuku AR, Ohenhen SO, Ejiofor N (1981) Nutrient composition of millet grains and malts. J Agric Food Chem 29:1247

    Article  Google Scholar 

  • Preet K, Punia D (2000) Proximate composition, phytic acid, polyphenols and digestibility (in vitro) of four brown cowpea varieties. Int J Food Sci Nutr 51:189–193

    Article  CAS  Google Scholar 

  • Savelkoul FH, Vander Poel AF, Tamminga S (1992) The presence and inactivation of trypsin inhibitors, tannins, lectins and amylase inhibitors in legume seeds during germination A review. Plant Foods Hum Nutr 42:71–85

    Article  CAS  Google Scholar 

  • Sharma S, Saxena AK, Bakshi AK, Brar JS (2007) Evaluation of different mungbean (vigna radiata) genotypes for physico-chemical and cooking quality characteristics. Indian J Nutr Diet 44:197–202

    CAS  Google Scholar 

  • Singh U, Jambunathan R (1981) Studies on desi and kabuli chickpea (cicer arietimun L.) cultivars: levels of protease inhibitors, levels of polyphenolic compounds and in vitro protein digestibility. J Food Sci 46:1364–1367

    Article  CAS  Google Scholar 

  • Singh U, Kherdekor MS, Jambunathan R (1982) Studies on desi and kabuli chickpea cultivars the levels of amylase inhibitors, level of oligosaccharides and in vitro starch digestibility. J Food Sci 47:510–512

    Article  CAS  Google Scholar 

  • Sood M, Malhotra SR, Sood BC (2002) Effect of processing and cooking on proximate composition of chickpea (Cicer arietinum) varieties. J Food Sci Technol 39:69–71

    Google Scholar 

  • Stephens JM (2003) Bean sprouts- Phaseolus aureus R. and Glycine max. Series no. HS557, Horticulture Sciences Department, Florida Cooperative Extension Service, Institute of Food Agricultural Sciences, University of Florida, US pp 1–2

  • Trugo LC, Donangele CM, Trugo NM, Bach Knudsen KE (2000) Effect oh heat treatment on nutritional quality of germinated legume seeds. J Agric Food Chem 48:2082–2086

    Article  CAS  Google Scholar 

  • Ziegler P (1995) Carbohydrate degradation during germination. In: Kigel J, Galili G (eds) Seed development and germination. Marcel Dekker Inc, New York, pp 447–474

    Google Scholar 

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Correspondence to Kiran Bains.

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Uppal, V., Bains, K. Effect of germination periods and hydrothermal treatments on in vitro protein and starch digestibility of germinated legumes. J Food Sci Technol 49, 184–191 (2012). https://doi.org/10.1007/s13197-011-0273-8

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  • DOI: https://doi.org/10.1007/s13197-011-0273-8

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