Abstract
Interleukin 10 (IL10) is a poor prognostic marker in several cancers. Its role in breast cancer is not well elucidated. The present study is designed to see the expression of IL10 in breast cancer tissue and to evaluate its correlation with the established markers of prognosis. Sixty female patients who underwent surgery for breast cancer were enrolled for the study. Immediately after surgery, 2–5 g of tumour tissue and similar volume of peritumoural normal breast tissue were collected for IL10 assay. IL10 expression was assayed by immunohistochemistry. IL10 expressing tumours and IL10 non expressing tumours were compared. Chi square/Fisher exact test and student’s t test were used to compare the data. p- valueless than 0.05 was considered as statistically significant. Thirty six patients (60 %) of carcinoma breast showed IL 10 expression in tumour tissue as compared to no IL 10 expression in any peritumouralnormal breast tissue (p < 0.01). IL10 expression had statistically significant correlation with locally advanced disease, tumour grade, HER2 + ve tumours and ER-ve, PR-ve, HER2 + ve breast cancer subtypes (p = 0.001, 0.001, 0.001 and 0.01 respectively). No correlation could be found with patient’s age, tumour size, tumour histology and ER and PR status. Correlation of IL10 expressing tumours with several established poor prognostic markers of breast cancer may indicate the possible association of IL10 expression with poor prognosis. Large studies with long term follow up are needed to substantiate the association of IL10 with poor prognosis.
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DeSantis C, Ma J, Bryan L, Jemal A (2014) Breast cancer statistics, 2013. CA Cancer J Clin 64:52–62
Hicks D, Turner B (2015) Pathologic diagnosis, immunohistochemistry, multigene assays and breast cancer treatment: progress toward"precision" cancer therapy. Biotech Histochem. 90:81–92
Wang J, Ding Q, Shi Y, et al. (2012) The interleukin-10-1082 promoter polymorphism and cancer risk: a meta-analysis. Mutagenesis 27:305–312
Lippitz BE (2013) Cytokine patterns in patients with cancer: a systematic review. Lancet Oncol 14:e218–e228
Hamidullah, Changkija B, Konwar R (2012) Role of interleukin-10 in breast cancer. Breast Cancer Res Treat 133:11–21
Mocellin S, Marincola F, Rossi CR, Nitti D, Lise M (2004) The multifaceted relationship between IL10 and adaptive immunity: putting together the pieces of a puzzle. Cytokine Growth Factor Rev 15:61–76
Huang S, Ullrich SE, Bar-Eli M (1999) Regulation of tumor growth and metastasis by interleukin-10: the melanoma experience. J Interf Cytokine Res 19:697–703
Venetsanakos E, Beckman I, Bradley J, Skinner JM (1997) High incidence of interleukin 10 mRNA but not interleukin 2 mRNA detected in human breast tumours. Br J Cancer 75:1826–1830
Llanes-Fernández L, Alvarez-Goyanes RI, Arango-Prado Mdel C, et al. (2006) Relationship between IL-10 and tumor markers in breast cancer patients. Breast 15:482–489
Chavey C, Bibeau F, Gourgou-Bourgade S, et al. (2007) Oestrogen receptor negative breast cancers exhibit high cytokine content. Breast Cancer Res 9:R15
Heckel MC, Wolfson A, Slachta CA, et al. (2011) Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70. Cell Immunol 266:143–153
Kozłowski L, Zakrzewska I, Tokajuk P, Wojtukiewicz MZ (2003) Concentration of interleukin-6 (IL-6), interleukin-8 (IL-8) and interleukin-10 (IL-10) in blood serum of breast cancer patients. Rocz Akad Med Bialymst 48:82–84
Lyon DE, McCain NL, Walter J, Schubert C (2008) Cytokine comparisons between women with breast cancer and women with a negative breast biopsy. Nurs Res 57:51–58
Eberl MM, Fox M, Edge CH, Carter SB, C, Mahoney, M. C (2006) BI-RADS classification for management of abnormal mammograms. J Am Board Fam Med 19:161–164
Eisenhauer EA, Therasse P, Bogaerts J, et al. (2009) New response evaluation criteria in solid tumours: revised RECIST guideline (version 1.1). Eur J Cancer 45:228–247
Ghosh R, Sharma A, Mitra DK, Agarwal SK, Dinda AK, Saxena A (2013) Study of CC chemokine receptor 5 in renal allograft rejection. Indian J Nephrol 23:196–200
Onitilo AA, Engel JM, Greenlee RT, Mukesh BN (2009) Breast cancer subtypes based on ER/PR and Her2 expression: comparison of clinicopathologic features andsurvival. Clin Med Res 7:4–13
Son KS, Kang HS, Kim SJ, et al. (2010) Hypomethylation of the interleukin-10 gene in breast cancer tissues. Breast 19:484–488
Toomey D, Harmey J, Condron C, Kay E, Bouchier-Hayes D (1999) Phenotyping of immune cell infiltrates in breast and colorectal tumours. Immunol Investig 28:29–41
Halak BK, Maguire HC Jr, Lattime EC (1999) Tumor-induced interleukin-10 inhibits type 1 immune responses directed at a tumor antigen as well as a non-tumor antigen present at the tumor site. Cancer Res 59:911–917
Sabat R (2010) IL-10 family of cytokines. Cytokine Growth Factor Rev 21(5):315–324
Ross JS, Slodkowska EA, Symmans WF, Pusztai L, Ravdin PM, Hortobagyi GN (2009) The HER-2 receptor and breast cancer: ten years of targeted anti-HER-2 therapy and personalized medicine. Oncologist 14:320–368
Slamon DJ, Clark GM, Wong SG, Levin WJ, Ullrich A, McGuire WL (1987) Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neuoncogene. Sci 235:177–182
Figueroa-Magalhães MC, Jelovac D, Connolly RM, Wolff AC (2014) Treatment of HER2-positive breast cancer. Breast 23:128–136
Su Y, Zheng Y, Zheng W, et al. (2011) Distinct distribution and prognostic significance of molecular subtypes of breast cancer in Chinese women: apopulation-based cohort study. BMC Cancer 11:292
Puig-Vives M, Sánchez MJ, Sánchez-Cantalejo J, et al. (2013) Distribution and prognosis of molecular breast cancer subtypes defined by immunohistochemical biomarkers in a Spanish population-based study. Gynecol Oncol 130:609–614
Kim MJ, Ro JY, Ahn SH, Kim HH, Kim SB, Gong G (2006) Clinicopathologic significance of the basal-like subtype of breast cancer: a comparison with hormone receptorand Her2/neu-overexpressing phenotypes. Hum Pathol 37:1217–1226
O’Brien KM, Cole SR, Tse CK, et al. (2010) Intrinsic breast tumor subtypes, race, and long-term survival in the Carolina Breast Cancer study. Clin Cancer Res 16:6100–6110
WJl J, HX J, HY F, YP Y, Chen K, FX S (2014) HER2-enriched tumors have the highest risk of local recurrence in Chinese patients treated with breast conservation therapy. Asian Pac J Cancer Prev 15:315–320
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Bhattacharjee, H.K., Bansal, V.K., Nepal, B. et al. Is Interleukin 10 (IL10) Expression in Breast Cancer a Marker of Poor Prognosis?. Indian J Surg Oncol 7, 320–325 (2016). https://doi.org/10.1007/s13193-016-0512-6
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DOI: https://doi.org/10.1007/s13193-016-0512-6