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miR-455-5p regulates atrial fibrillation by targeting suppressor of cytokines signaling 3

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Abstract

Atrial fibrillation (AF) is a condition that heart beats quaveringly or irregularly, which causes blood clots, heart failure, stroke, and other heart-related complications. Therefore, early diagnosis and timely preventions are necessary for AF treatment. Compelling evidence indicated that microRNAs (miRNAs) become emerging biomarkers of AF; thus, we aimed to investigate the possibility of miR-455-5p as an AF marker to provide a new strategy for early diagnosis of AF. A minipump containing angiotensin II was implanted into mice to induce AF, and adeno-associated virus (AAV) carrying anti-miR-negative control (NC) or anti-miR-455-5p was injected into the pericardial space of mice respectively. Next, myocytes isolated from wild-type newborn mice were stimulated with angiotensin II and anti-miR-NC or anti-miR-455-5p mimic. The results showed that the expression of miR-455-5p was positively correlated with the severity of AF, and miR-455-5p mimic accelerated the progression of AF by directly binding to its target gene suppressor of cytokines signaling 3 (SOCS3), leading to the activation of signal transducer and activator of transcription 3 (STAT3) signaling pathway. On the contrary, inhibition of miR-455-5p expression effectively ameliorated AF. In conclusion, miR-455-5p might serve as a biomarker of AF.

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Funding

This work was supported by the departmental funding.

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Authors and Affiliations

  1. The Fourth Hospital of Shijiazhuang, Shijiazhuang, 050000, Hebei, China

    Weiling Li

  2. Department of Respiratory and Critical Care Medicine, Hengshui People Hospital, Hengshui, 053000, China

    Na Qi

  3. Department of Cardiology, Shijiazhuang General Hospital, No. 9 Fangbei Road, Shijiazhuang, 050000, Hebei, China

    Shuo Wang

  4. Department of Cardiology, Tangshan People’s Hospital, Tangshan, 063000, Hebei, China

    Wenyan Jiang

  5. Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China

    Tao Liu

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  1. Weiling Li
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  2. Na Qi
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  5. Tao Liu
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Correspondence to Tao Liu.

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Key Points

1. The expression of miR-455-5p was positively correlated with the severity of AF.

2. Inhibition of miR-455-5p expression effectively ameliorated AF.

3. miR-455-5p might serve as a biomarker of AF.

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Li, W., Qi, N., Wang, S. et al. miR-455-5p regulates atrial fibrillation by targeting suppressor of cytokines signaling 3. J Physiol Biochem 77, 481–490 (2021). https://doi.org/10.1007/s13105-021-00808-x

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  • DOI: https://doi.org/10.1007/s13105-021-00808-x

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