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Exercise training and high-fat diet elicit endocannabinoid system modifications in the rat hypothalamus and hippocampus

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An Erratum to this article was published on 05 October 2017

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Abstract

The purpose of the present study was to examine the effect of chronic exercise on the hypothalamus and hippocampus levels of the endocannabinoids (eCBs) anandamide (AEA) and 2-arachidonoylglycerol (2-AG) and of two AEA congeners and on the expression of genes coding for CB1, CB2 receptors (Cnr1 and Cnr2, respectively), and the enzymes responsible for eCB biosynthesis and degradation, in rats fed with a standard or high-fat diet. Male Wistar rats (n = 28) were placed on a 12-week high-fat (HFD) or standard diet period, followed by 12 weeks of exercise training for half of each group. Tissue levels of eCBs and related lipids were measured by liquid chromatography mass spectrometry, and expression of genes coding for CB1 and CB2 receptors and eCB metabolic enzymes was measured by quantitative real-time polymerase chain reaction (qPCR). HFD induced a significant increase in 2-AG (p < 0.01) in hypothalamus. High-fat diet paired with exercise training had no effect on AEA, 2-AG, and AEA congener levels in the hypothalamus and hippocampus. Cnr1 expression levels were significantly increased in the hippocampus in response to HFD, exercise, and the combination of both (p < 0.05). Our results indicate that eCB signaling in the CNS is sensitive to diet and/or exercise.

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Change history

  • 05 October 2017

    Volume 73 issue 3 was published with an incorrect cover date. Correct is August 2017. The Publisher apologizes for this mistake and all related inconveniences caused by this.

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Acknowledgments

The authors thank Gamain J., Barbez P., Max, and Mahault G. for their advices and technical assistances.

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Authors and Affiliations

  1. Univ Lille Nord de France, F-59000, Lille, France

    François-Xavier Gamelin, Julien Aucouturier, Melissa Leriche, Erwan Dupont, Caroline Cieniewski-Bernard, Erwan Leclair, Bruno Bastide & Elsa Heyman

  2. Equipe dʼAccueil 7369, URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société - Equipe Activité Physique, Muscle, Santé, Eurasport, 413 rue Eugène Avinée, 59120, Loos, France

    François-Xavier Gamelin, Julien Aucouturier, Melissa Leriche, Erwan Dupont, Caroline Cieniewski-Bernard, Erwan Leclair, Bruno Bastide & Elsa Heyman

  3. CNR, Endocannabinoid Research Group, Institute of Biomolecular Chemistry, I-80078, Pozzuoli, Italy

    Fabio Arturo Iannotti, Fabiana Piscitelli, Enrico Mazzarella, Teresa Aveta & Vincenzo Di Marzo

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  1. François-Xavier Gamelin
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Correspondence to François-Xavier Gamelin.

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Vincenzo Di Marzo, and Elsa Heyman share the senior authorship.

An erratum to this article is available at https://doi.org/10.1007/s13105-017-0593-x.

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Gamelin, FX., Aucouturier, J., Iannotti, F.A. et al. Exercise training and high-fat diet elicit endocannabinoid system modifications in the rat hypothalamus and hippocampus. J Physiol Biochem 73, 335–347 (2016). https://doi.org/10.1007/s13105-017-0557-1

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