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Tissue culture and micropropagation of tree peony (Paeonia suffruticosa Andr.)

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Abstract

Tree peony (Paeonia suffruticosa) is one of China’s most traditional flowers. It is also one of the most remarkable flowering ornamentals with a rich history of breeding. The speed and success of propagation have always been a major concern for breeders and cultivators. Tissue culture is one of the few effective methods to overcome these inherent difficulties. This article reviews advances made on various aspects of tree peony tissue culture and micropropagation, including the in vitro culture of buds, stem apex, leaves and petioles, sexual embryos and ovules, somatic embryos, anther and callus. Since the vast majority of literature is published in Chinese, this review provides a unique and valuable resource and spring-board from which to initiate or further studies related to in vitro culture of tree peony.

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Abbreviations

ABST:

axillary bud stem tip

AC:

active charcoal

BA:

N6-benzyl adenine

B5:

B5 medium (Gamborg et al. 1968)

CCFL:

cold cathode fluorescent lamp

CH:

casein hydrolysate

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3:

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KT:

kinetin

LED:

light-emitting diode

LH:

lactoalbumin hydrolysate

MS:

Murashige and Skoog medium (1962)

NAA:

1-naphthyleneacetic acid

PGR:

plant growth regulator

PVP:

polyvinylpyrrolidone

RIM:

root induction medium

TDZ:

thidiazuron

VC:

vitamin C

WPM:

Woody Plant Medium (Lloyd and McCown 1980)

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Authors and Affiliations

  1. Miki-cho, Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Ikenobe 2393, Kagawa-ken, 761-0795, Japan

    Jaime A. Teixeira da Silva

  2. College of Landscape Architecture, Beijing Forestry University, No. 35 Qinghuadong Road, Beijing, 100083, P. R. China

    Miaomiao Shen & XiaoNan Yu

  3. National Engineering Research Center for Floriculture, No. 35 Qinghuadong Road, Beijing, 100083, P. R. China

    XiaoNan Yu

Authors
  1. Jaime A. Teixeira da Silva
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  2. Miaomiao Shen
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  3. XiaoNan Yu
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Correspondence to XiaoNan Yu.

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Teixeira da Silva, J.A., Shen, M. & Yu, X. Tissue culture and micropropagation of tree peony (Paeonia suffruticosa Andr.). J. Crop Sci. Biotechnol. 15, 159–168 (2012). https://doi.org/10.1007/s12892-012-0040-z

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  • DOI: https://doi.org/10.1007/s12892-012-0040-z

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