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Microsatellites for the at-risk Mottled Duskywing butterfly, Erynnis martialis

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Abstract

We describe 24 microsatellite loci for an at-risk North American butterfly, the Mottled Duskywing (Erynnis martialis). Forty-one perfect tetra and di-nucleotide loci were originally identified using Illumina sequencing and were refined to 24 variable and cleanly amplifiable loci, which we attempted to characterized in 34 individuals from a single population. We detected 2 to 10 alleles per locus. Observed heterozygosity ranged from 0.06 to 0.76, and expected heterozygosity ranged from 0.06 to 0.79. We also describe conditions by which 18 microsatellites can be multiplexed in five separate reactions. These microsatellites will be used to investigate population differentiation and diversity of Mottled Duskywing, to inform reintroduction and conservation protocols for the species in Ontario, Canada where it is listed as endangered.

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References

  • COSEWIC (2015) Recovery strategy for the mottled Duskywing (Erynnis martialis) in Ontario. Ontario Ministry of Natural Resources and Forestry. Ontario Recovery Strategy Series, Canada. https://collections.ola.org/mon/29008/331486.pdf. Accessed 27 Mar, 2020

  • Koscinski D, Crawford LA, Keller HA, Keyghobadi N (2011) Effects of different methods of non-lethal tissue sampling on butterflies. Ecol Entomol 36:301–308

    Article  Google Scholar 

  • Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2009) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. J Bioinform 26:403–404

    Article  Google Scholar 

  • Rousset F (2008) GENEPOP ’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Van Oosterhout C, Hutchinson WFD, Wills DP, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

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Acknowledgements

This work was supported by the efforts of the Ontario Butterfly Species at Risk Recovery Team.

Funding

Funding was provided by an NSERC Alliance grant, NSERC Engage grant, and NSERC Canada Graduate Scholarship (Masters).

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Authors and Affiliations

  1. Department of Biology, Western University, London, ON, Canada

    Shayla L. Kroeze & Nusha Keyghobadi

  2. Cambridge Butterfly Conservatory, Cambridge, ON, Canada

    Adrienne Brewster & Yvonne Young

  3. Natural Resource Solutions Incorporated, Waterloo, ON, Canada

    Jessica E. Linton

  4. Department of Integrative Biology, University of Guelph, Guelph, ON, Canada

    D. Ryan Norris

  5. School of Environmental Sciences, University of Guelph, Guelph, ON, Canada

    Gard W. Otis

Authors
  1. Shayla L. Kroeze
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  2. Adrienne Brewster
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  3. Jessica E. Linton
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  4. D. Ryan Norris
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  5. Gard W. Otis
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  6. Yvonne Young
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  7. Nusha Keyghobadi
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Contributions

SLK conducted laboratory work, optimized non-lethal sampling, analyzed data, and wrote the manuscript under the supervision of NK, with input and contributions from GWO. JEL oversaw field collections, while AB and YY collected and housed whole females. JEL, DRN, AB and NK contributed to funding acquisition, development, and administration of the project. All authors reviewed and approved the manuscript.

Corresponding author

Correspondence to Shayla L. Kroeze.

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The authors have not disclosed any competing interests.

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Kroeze, S.L., Brewster, A., Linton, J.E. et al. Microsatellites for the at-risk Mottled Duskywing butterfly, Erynnis martialis. Conservation Genet Resour 14, 387–388 (2022). https://doi.org/10.1007/s12686-022-01280-5

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  • DOI: https://doi.org/10.1007/s12686-022-01280-5

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