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A conservation-oriented SNP panel for Smallmouth Bass (Micropterus dolomieu), with emphasis on Interior Highlands lineages

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Abstract

The Smallmouth Bass (Micropterus dolomieu; SMB) is a widely distributed black bass species, but the southwestern edge of the species range within the Interior Highlands contains some of the most divergent ecotypes. The Neosho subspecies (M. d. velox) inhabits tributaries of the Arkansas River within the Ozark Mountains and a second lineage is reported from drainages of the Ouachita Mountains. We sought to develop a single nucleotide polymorphism (SNP) panel to (1) diagnose hybridization with sympatric Spotted Bass (Micropterus punctulatus; SPB) and non-native Northern SMB (M. d. dolomieu) stocked in the region, and (2) delineate population structure within the ranges of the Neosho and Ouachita SMB lineages. We obtained 76 individual SMB samples from across their range but concentrated within the Interior Highlands (n = 50). We also included 3 SPB to allow for hybrid detection and 3 Shoal Bass (Micropterus cataractae) as an outgroup. Phylogenetic trees constructed with the generated SNP data corroborated the existence of at least three major lineages of SMB (Northern, Neosho, and Ouachita), each containing varying degrees of differentiation among major drainages. Simulation analyses revealed that chosen SNPs had high power (> 0.9) to assign SMB × SPB hybrid categories and similarly high power (> 0.8) for Northern SMB × Interior Highlands SMB hybrids. Clustering methods delineated major inter-basin population structure within the native ranges of Neosho and Ouachita SMB with chosen SNPs. Anticipated uses of the resulting 192-loci SNP panel include conservation planning, fisheries management assessments, and ecological investigations of the Neosho and Ouachita SMB lineages.

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Acknowledgements

For contributing fin clips: Jeff Buckingham, Aaron Kern, John Deller, Dray Carl, Mark Rogers, Ashley Nealis, Randy Jackson, Colt Holley, and Joel Bjornen. For constructive review prior to publication, we thank M. Lewis. This study was performed, in part, under the auspices of Oklahoma State University’s Institutional Animal Care and Use Committee’s Protocol # AG-13-8. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Funding was provided by the Oklahoma Cooperative Fish and Wildlife Research Unit (U.S. Geological Survey, Oklahoma State University, Oklahoma Department of Wildlife Conservation, Wildlife Management Institute, and U.S. Fish and Wildlife Service, cooperating).

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  1. Andrew T. Taylor

    Present address: Department of Biology, University of Central Oklahoma, Edmond, OK, 73034, USA

Authors and Affiliations

  1. U.S. Geological Survey, Oklahoma Cooperative Fish and Wildlife Research Unit, Department of Natural Resource Ecology and Management, Oklahoma State University, 007 Agriculture Hall, Stillwater, OK, 74078, USA

    James M. Long

  2. Oklahoma Cooperative Fish and Wildlife Research Unit, Department of Natural Resource Ecology and Management, Oklahoma State University, 007 Agriculture Hall, Stillwater, OK, 74078, USA

    Andrew T. Taylor

  3. The Center for Aquaculture Technologies, 8395 Camino Santa Fe, Suite E, San Diego, CA, 92121, USA

    Vince Buonaccorsi

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Correspondence to James M. Long.

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Long, J.M., Taylor, A.T. & Buonaccorsi, V. A conservation-oriented SNP panel for Smallmouth Bass (Micropterus dolomieu), with emphasis on Interior Highlands lineages. Conservation Genet Resour 13, 47–59 (2021). https://doi.org/10.1007/s12686-020-01170-8

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