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Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst’s frog (B. holsti)

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Abstract

The Otton frog (Babina subaspera) and Holst’s frog (Babina holsti) are both endangered sister species belonging to family Ranidae. For extensive genetic and ecological research of these species, we isolated and characterized 8 new microsatellite loci of the Otton frog and validated cross-amplification in Holst’s frog along with 8 previously reported loci. The total number of alleles and the expected heterozygosity of newly isolated loci in the Otton frog population ranged from 5 to 12 and from 0.620 to 0.905, respectively. We also confirmed cross-amplification in 4 of the new loci and in all previously reported loci in Holst’s frog with the same level of polymorphism as the Otton frog. Our findings suggest that these novel loci will be applicable for conservation genetic studies across varying scales.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (B and C) (No. 24310173 and No. 20510216) to MS and a Grant-in-Aid for Young Scientists (B) (No. 23710282) to TI from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Graduate School of Science, Institute for Amphibian Biology, Hiroshima University, Hiroshima, 739-8526, Japan

    Ryosuke Kakehashi, Takeshi Igawa & Masayuki Sumida

  2. Graduate School of Agricultural and Life Sciences, Ecohydrology Research Institute, The University of Tokyo, Goizuka Seto, Aichi, 489-0031, Japan

    Noriko Iwai

  3. Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki, 305-8687, Japan

    Etsuko Shoda-Kagaya

Authors
  1. Ryosuke Kakehashi
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  2. Takeshi Igawa
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  3. Noriko Iwai
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  4. Etsuko Shoda-Kagaya
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  5. Masayuki Sumida
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Corresponding author

Correspondence to Masayuki Sumida.

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Kakehashi, R., Igawa, T., Iwai, N. et al. Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst’s frog (B. holsti). Conservation Genet Resour 5, 1071–1073 (2013). https://doi.org/10.1007/s12686-013-9981-x

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  • DOI: https://doi.org/10.1007/s12686-013-9981-x

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