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Isolation, via 454 sequencing, characterization and transferability of microsatellites for Grevillea thelemanniana subsp. thelemanniana and cross-species amplification in the Grevillea thelemanniana complex (Proteaceae)

  • Microsatellite Letters
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Abstract

A set of 12 polymorphic microsatellite markers were developed for Grevillea thelemanniana subsp. thelemanniana, a rare and threatened shrub, endemic to southwest Western Australia. Genomic sequences were obtained from next generation (454) sequencing in the target species. Primer pairs for a total of 30 microsatellite loci were designed from these of which 13 were successfully amplified in 25 individuals from Kenwick Nature Reserve, Perth. Twelve loci were polymorphic with observed heterozygosity ranging from 0.12 to 0.6 and the number of alleles per locus ranged from 2 to 8. These markers were trialed in all species within the G. thelemanniana species complex. Amplification success and level of polymorphism varied among loci and taxa, but most (96 %) were successful. These loci will be useful in understanding the genetic variation, molecular ecology and phylogenetic relationships in the G. thelemanniana complex.

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Authors and Affiliations

  1. School of Plant Biology, University of Western Australia, 35 Stirling Ave, Crawley, WA, 6009, Australia

    Tanya H. Hevroy

  2. Department of Biological Sciences, University of Texas at El Paso, 500 W. University Ave., El Paso, TX, 79968, USA

    Michael L. Moody

  3. Kings Park and Botanic Garden, Botanic Gardens and Parks Authority, Fraser Ave, West Perth, WA, 6005, Australia

    Siegfried L. Krauss

  4. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, 5001, SA, Australia

    Michael G. Gardner

  5. Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, 5005, Adelaide, Australia

    Michael G. Gardner

  6. Evolutionary Biology Unit South Australian Museum, Nth Tce, Adelaide, SA, 5001, Australia

    Michael G. Gardner

Authors
  1. Tanya H. Hevroy
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  2. Michael L. Moody
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  3. Siegfried L. Krauss
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  4. Michael G. Gardner
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Correspondence to Tanya H. Hevroy.

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Hevroy, T.H., Moody, M.L., Krauss, S.L. et al. Isolation, via 454 sequencing, characterization and transferability of microsatellites for Grevillea thelemanniana subsp. thelemanniana and cross-species amplification in the Grevillea thelemanniana complex (Proteaceae). Conservation Genet Resour 5, 887–890 (2013). https://doi.org/10.1007/s12686-013-9918-4

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  • DOI: https://doi.org/10.1007/s12686-013-9918-4

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