Abstract
We describe a microsatellite panel of 12 newly developed and four cross-amplification loci for the endangered Australian sea lion, Neophoca cinerea. Next-generation 454 sequencing was used to obtain species-specific partial genomic library. We genotyped 28 individuals sampled from a single breeding colony to characterize these 16 polymorphic loci. Number of alleles per locus ranged from three to seven, and observed and expected heterozygosites from 0.333 to 0.852 and from 0.377 to 0.787, respectively. These markers will be used to examine genetic diversity, genetic connectivity among colonies and the mating system of Australian sea lions.
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Acknowledgments
This work was funded by Australian Marine Mammal Centre, Department of Environment, Heritage SA and WA, Water and the Arts through the Commonwealth Environment Research Fund. We would like to thank Benjamin Pitcher and Isabelle Charrier for their assistance in the field, Jessica Boomer, Siobhan Dennison and Heather Baldwin for laboratory assistance and Mike Gardner for assistance with 454 sequencing run.
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Ahonen, H., Lowther, A.D., Goldsworthy, S.D. et al. Characterization of 12 novel microsatellite loci and cross-amplification of four loci in the endangered Australian sea lion (Neophoca cinerea). Conservation Genet Resour 5, 283–285 (2013). https://doi.org/10.1007/s12686-012-9788-1
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DOI: https://doi.org/10.1007/s12686-012-9788-1