Abstract
The clingfish, Lepadogaster lepadogaster is a reef fish species, abundant in temperate nearshore rocky reefs of the Eastern Atlantic and central and Eastern Mediterranean. To study genetic variability and population connectivity of this species, we developed fifteen polymorphic microsatellite markers. These were tested in one population and all but one, showed no departure from Hardy–Weinberg equilibrium. Average overall observed heterozygosity was 0.66 and allelic richness was 8.9. Two primer pairs revealed possible linkage disequilibrium. These markers open perspectives for population genetic studies of this species to unravel connectivity and population biology, vital information for future conservation studies.
References
Almany GR, Berumen ML, Thorrold SR, Planes S, Jone GP (2007) Local replenishment of coral reef fish populations in a marine reserve. Science 316:742–744
Beldade R, Pedro T, Gonçalves EJ (2007) Pelagic larval duration of 10 temperate cryptobenthic fishes. J Fish Biol 71:376–382
Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (1996–2004) GENETIX 4.05, logiciel sous Windows™ pour la génétique des populations. Laboratoire Génome, Populations, Interactions, CNRS UMR 5000, Université de Montpellier II, Montpellier (France)
Black WC, Krafsur ES (1985) A FORTRAN program for the calculation and analysis of two-locus linkage disequilibrium coefficients. Theor App Genet 70:491–496
Borges R, Ben-Hamadou RR, Chícharo MA, Ré P, Gonçalves EJ (2007) Horizontal spatial and temporal patterns of nearshore larval fish assemblages at a temperate rocky shore. Estuar Coast Shelf Sci 71:412–428
Briggs JC (1955) A monograph of the clingfishes (order Xenopterygii). Stanf Ichthyol Bull 6:1–224
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Faircloth BC (2008) Msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Faria A, Gonçalves E (2010) Ontogeny of swimming behaviour of two temperate clingfishes, Lepadogaster lepadogaster and Lepadogaster purpurea (Gobiesocidae). Mar Ecol Progr Ser 414:237–248
Gonçalves D, Gonçalves EJ, Almada VC, Almeida SP (1998) Comparative behaviour of two species of Lepadogaster (Pisces: Gobiesocidae) living at different depths. J Fish Biol 53:447–450
Henriques M, Lourenço R, Almada F, Calado G, Gonçalves DM, Guillemaud T, Cancela ML, Almada VC (2002) A revision of the status of Lepadogaster lepadogaster (Teleostei: Gobiesocidae): sympatric subspecies or a long misunderstood blend of species? Biol J Linn Soc 76:327–338
Jones GP, Planes S, Thorrold SR (2005) Coral reef fish larvae settle close to home. Curr Biol 15:1314–1318
Leis JM (2006) Are larvae of demersal fishes plankton or nekton? Adv Mar Biol 51:59–141
Leis J, Siebeck U, Dixson DL (2011) How nemo finds home: the neuroecology of dispersal and of population connectivity in larvae of marine fishes. Integr Comp Biol 51:826–843
Quetglas A (2000) Effects of marine protected areas on recruitment processes with special reference to Mediterranean littoral ecosystems. Environ Conserv 27:126–143
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schuelke M (2000) An economic method for the fluorescent labelling of PCR fragments. Nat Biotech 18:233–234
Tojeira I, Faria AM, Henriques S, Faria C, Gonçalves EJ (2012) Early development and larval behaviour of two clingfishes, Lepadogaster purpurea and Lepadogaster lepadogaster (Pisces: Gobiesocidae). Environ Biol Fish 93:449–459
Van Oosterhout C, Hutchinson W, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 2:377–379
Acknowledgments
This study was supported by the Portuguese Science Foundation (FCT) through the project MATRIX (PTDC/MAR/115226/2009) and the fellowships SFRH/BPD/39097/2007 (to ST) SFRH/BPD/23743/2005 (to RB) and SFRH/BD/72970/2010 (to MK).
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Teixeira, S., Candeias, R., Klein, M. et al. Characterization of 15 polymorphic microsatellite loci in the temperate reef fish Lepadogaster lepadogaster, developed using 454-sequencing. Conservation Genet Resour 5, 55–57 (2013). https://doi.org/10.1007/s12686-012-9732-4
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DOI: https://doi.org/10.1007/s12686-012-9732-4