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Isolation and characterization of 14 novel polymorphic loci for the Florida mouse (Podomys floridanus)

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Abstract

We used 454 shotgun sequencing to locate microsatellite loci for use in molecular studies of the Florida mouse (Podomys floridanus). A total of 9,350 microsatellites were detected, from which a total of 23 were selected for screening and characterization. Of the initial 23 markers, 14 were retained based on amplification success and ease of scoring and further screened on 49 Florida mice sampled at four adjacent management units. The number of alleles per locus ranged from 4 to 12, with mean observed and expected heterozygosity values of 0.605 and 0.741, respectively. Three loci showed evidence of null alleles at both the management unit level and across combined samples, while two additional loci deviated from HWE when samples were pooled. No loci exhibited significant linkage disequilibrium.

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Acknowledgments

We thank J. Irwin, J. Townsend, I. Luque-Montes, and A. Smith for assistance with sample collection. Funding for marker development was provided by the School of Natural Resources & Environment at UF. Sampling occurred under permit number LSSC-10-00073.

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Authors and Affiliations

  1. Department of Wildlife Ecology and Conservation, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110430, Gainesville, FL, 32611, USA

    John Hargrove & James D. Austin

  2. Department of Botany, University of Florida, P.O. Box 118526, Gainesville, FL, 32611, USA

    Matt Gitzendanner

  3. Program in Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, Institute of Food and Agricultural Sciences, University of Florida, 7922 NW 71st St, Gainesville, FL, 32653, USA

    James D. Austin

Authors
  1. John Hargrove
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  2. Matt Gitzendanner
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  3. James D. Austin
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Correspondence to John Hargrove.

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Hargrove, J., Gitzendanner, M. & Austin, J.D. Isolation and characterization of 14 novel polymorphic loci for the Florida mouse (Podomys floridanus). Conservation Genet Resour 4, 411–413 (2012). https://doi.org/10.1007/s12686-011-9562-9

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  • DOI: https://doi.org/10.1007/s12686-011-9562-9

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