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Microsatellite loci for testing temporal changes in the population genetics of the Florida mouse (Podomys floridanus)

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Abstract

The Florida mouse (Podomys floridanus) is endemic to Florida and is considered vulnerable by the IUCN due to extensive habitat loss. Here, we describe a panel of 76 microsatellite DNA markers for population genetic studies of this species. Fourteen of these loci were examined to estimate genetic diversity for 39 specimens collected in Florida in 1957 and 2006. DNA extractions from the 1957 specimens were carried out from snips of skin taken from museum voucher skins, whereas the 2006 samples were extracted from fresh liver tissue. The use of museum skins for reconstructing the genetics of historical populations is becoming a critical part of understanding past, present, and future genetic trends for threatened species. Mean expected heterozygosity for the two populations was 0.73 and mean observed heterozygosity was 0.75. The number of alleles per locus ranged from 2 to 15. All microsatellites were polymorphic, and exhibited variability across time.

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Acknowledgments

We thank H. Hoesktra and J. Weber for help with primers and laboratory support; J. Austin, C. McCaffery, L. Harris, J. Weber, J. Pino, J. A. Soto-Centeno, L. Barrow, C. Spirson, B. Pasch, S. Gonzalez, D. Pearson and L. Ramos for help with information, laboratory support and field work.

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Authors and Affiliations

  1. Florida Museum of Natural History, University of Florida, Dickinson Hall, Museum Rd & Newell Dr., Gainesville, FL, 32611, USA

    Catalina Rivadeneira, Julie M. Allen & David L. Reed

  2. School of Natural Resources and the Environment, University of Florida, Gainesville, FL, 32611, USA

    Catalina Rivadeneira

  3. Department of Biology, University of Florida, Gainesville, FL, 32611, USA

    Julie M. Allen

Authors
  1. Catalina Rivadeneira
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  2. Julie M. Allen
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  3. David L. Reed
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Correspondence to David L. Reed.

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Rivadeneira, C., Allen, J.M. & Reed, D.L. Microsatellite loci for testing temporal changes in the population genetics of the Florida mouse (Podomys floridanus). Conservation Genet Resour 3, 135–139 (2011). https://doi.org/10.1007/s12686-010-9308-0

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  • DOI: https://doi.org/10.1007/s12686-010-9308-0

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