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Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus

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Abstract

This study employed the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the tropical sea cucumber species Stichopus horrens. Sixteen novel microsatellite loci were identified and we assessed the polymorphism of 40 individual samples from Hainan Province, China. The numbers of alleles ranged from 8 to 18 (total 213, mean 13.3), the observed and expected heterozygosity varied from 0.294 to 0.971, and from 0.720 to 0.913, and 12 of the 16 loci were found to be in accordance with the Hardy–Weinberg equilibrium. In addition, the successful cross-amplification of 8 loci with the congeneric Stichopus monotuberculatus, suggests that these markers could also be used in this species. These polymorphic markers can be useful for additional studies of population structure and stock management of S. horrens and related species.

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Acknowledgments

This work was funded by the National Key Technology R&D Program (2009BAB44B02), Guangdong Province and CAS Cooperation Program (2009B091300155) and Fishery Scientific and Technical Program of Guangdong Province (A200899E02, A200901E01, A201001E01) assigned to CQ Hu. LH Yuan is supported by the China Postdoctoral Science Foundation (20100480788). We greatefully thank the Deep Ocean Observation and Research Station on XiSha Islands (South China Sea institution of Oceanology, CAS) for the support on samples collection, Ms. C. Monagin for her helpful comments on the earlier version, and acknowledge the support of K.C. Wong Education Foundation, Hong Kong.

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Authors and Affiliations

  1. Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Lihong Yuan, Jianjun Xia, Lvping Zhang & Chaoqun Hu

  2. Key Laboratory of Applied Marine Biology of Guangdong Province (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Lihong Yuan, Jianjun Xia, Lvping Zhang & Chaoqun Hu

  3. South China Institute of Endangered Animals, Guangzhou, 510260, China

    Lihong Yuan

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  1. Lihong Yuan
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  2. Jianjun Xia
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  3. Lvping Zhang
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  4. Chaoqun Hu
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Correspondence to Chaoqun Hu.

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Yuan, L., Xia, J., Zhang, L. et al. Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus . Conservation Genet Resour 4, 303–305 (2012). https://doi.org/10.1007/s12686-011-9530-4

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  • DOI: https://doi.org/10.1007/s12686-011-9530-4

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