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Isolation of novel microsatellite markers for tambaqui (Colossoma macropomum, Cuvier 1818), an important freshwater fish of the Amazon

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Abstract

Colossoma macropomum is an endemic species from Amazon basin. It is widely commercialized as food, becoming one of the main items in Amazonian fisheries. Despite its economic importance, genetic information of small captivity populations is not currently available. The present contribution describes 14 new microsatellite loci used to analyze 30 individuals of C. macropomum. The number of alleles for each locus ranged from 4 to 24. The observed (Ho) and expected (H E ) heterozygosity values ranged from 0.318 to 1.000 and 0.729 to 0.949, respectively. Out of 14 polymorphic loci, nine did not deviate from Hardy–Weinberg Equilibrium after Bonferroni correction. These new microsatellite loci will contribute towards the genetic of small artificial populations, as well as pedigree control of fish farms of C. macropomum.

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Acknowledgments

The authors are grateful to the PIATAM Project for providing the samples collected from a wild population in lakes along Solimões river between the cities of Coari and Manaus. The authors would like to thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Amazonas (FAPEAM) and Conselho Nacional de Pesquisa (CNPq) for financial supports. CHAS is the recipient of a Ph.D. fellowship from CNPq. The Program for Academic Cooperation PROCAD between INPA/UNICAMP/UFRGS was supported by CAPES (no.023/2006/CAPES). VMFAV are the recipient of research fellowship from CNPq. This work is part of INCT ADAPTA, coordinated by Adalberto Luis Val.

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Authors and Affiliations

  1. Laboratório de Ecofisiologia e Evolução Molecular (LEEM), Instituto Nacional de Pesquisas da Amazônia (INPA), Aleixo, Manaus, AM, CEP 69060-001, Brazil

    G. X. Santana, C. H. A. Santos, C. F. S. Sousa, P. R. M. Nascimento, M. N. Paula-Silva & V. M. F. Almeida-Val

  2. Universidade do Estado da Bahia, Paulo Afonso, BA, 48608-230, Brazil

    A. C. B. Sousa

  3. Empresa Brasileira de Pesquisa Agropecuária, Rio Branco, AC, 69908-970, Brazil

    T. Campos

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  1. G. X. Santana
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  2. C. H. A. Santos
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  3. C. F. S. Sousa
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  4. P. R. M. Nascimento
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  5. M. N. Paula-Silva
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  6. A. C. B. Sousa
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  7. T. Campos
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  8. V. M. F. Almeida-Val
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Correspondence to C. H. A. Santos.

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Santana, G.X., Santos, C.H.A., Sousa, C.F.S. et al. Isolation of novel microsatellite markers for tambaqui (Colossoma macropomum, Cuvier 1818), an important freshwater fish of the Amazon. Conservation Genet Resour 4, 197–200 (2012). https://doi.org/10.1007/s12686-011-9507-3

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  • DOI: https://doi.org/10.1007/s12686-011-9507-3

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