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Isolation and characterization of tri and tetranucleotide microsatellite markers for the tambaqui (Colossoma macropomum, Serrasalmidae, Characiformes)

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Abstract

We isolated 13 tri and tetranucleotide microsatellite markers for the species Colossoma macropomum that can be used in management programmes for this species of Amazon fish. This panel of microsatellite markers was used in the genotyping of 20 individual specimens collected in the lakes of Óbidos city, in the Brazilian Amazon. The number of alleles per locus varied from four to ten. The observed heterozygosity varied from 0.31 to 0.95. We observed no significant deviation from the expected Hardy–Weinberg equilibrium assumption. In the sample investigated, it was not possible to identify any significant linkage disequilibrium among the 78 possible loci pairs. In our analysis, we found no indication of genotyping error attributed to stutter bands, large allele dropout or null alleles.

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Acknowledgments

This research was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Financiadora de Estudos e Projetos (FINEP).

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Authors and Affiliations

  1. Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Cidade Universitária Prof. José da Silveira Netto. Av. Augusto Corrêa, 01, Belém, PA, Brazil

    Igor Guerreiro Hamoy & Sidney Santos

  2. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil

    Fernanda Witt Cidade

  3. Laboratório de Polimorfismo de DNA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Cidade Universitária Prof. José da Silveira Netto. Av. Augusto Corrêa, 01, Belém, PA, Brazil

    Maria Silvanira Barbosa & Evonnildo Costa Gonçalves

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  1. Igor Guerreiro Hamoy
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  2. Fernanda Witt Cidade
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  3. Maria Silvanira Barbosa
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  4. Evonnildo Costa Gonçalves
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  5. Sidney Santos
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Correspondence to Igor Guerreiro Hamoy.

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Hamoy, I.G., Cidade, F.W., Barbosa, M.S. et al. Isolation and characterization of tri and tetranucleotide microsatellite markers for the tambaqui (Colossoma macropomum, Serrasalmidae, Characiformes). Conservation Genet Resour 3, 33–36 (2011). https://doi.org/10.1007/s12686-010-9275-5

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  • DOI: https://doi.org/10.1007/s12686-010-9275-5

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