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Direct Electrochemistry of Glucose Oxidase on a Three-Dimensional Porous Zirconium Phosphate–Carbon Aerogel Composite

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Abstract

Biocompatible materials with large specific surface areas can play a crucial role in direct electron transfer between redox proteins and an electrode surface. Here, we report zirconium phosphate–carbon aerogel (ZrP-CA) composites with a large specific surface area and uniform nanopore distributions as matrix for glucose oxidase immobilization. The immobilized glucose oxidase displays two stable, well-defined redox peaks with an electron transfer rate constant of 9.34 s−1 in nitrogen-saturated phosphate-buffered saline (PBS) solution (0.1 M, pH 7.0), at a scan rate of 100 mV s−1. The modified electrode was also used as a glucose biosensor, which was found to exhibit a linear calibration range of 0.12–2.0 mM, sensitivity of 5.56 μA mM−1 cm−2 at an applied potential of −0.5 V, and detection limit of 34 μM based on a signal-to-noise ratio of 3. After 80 scan cycles, the decreases in the peak current were less than 8 %, indicating good stability of the as-prepared ZrP-CA. The unique characteristics of the ZrP-CA nanocomposite make it a good matrix for protein immobilization and biosensor preparation.

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Acknowledgments

We gratefully acknowledge the General Program of Science and Technology Development Project of Beijing Municipal Education Commission of China (KM201210005008), National Natural Science Foundation of China (21275014), and the Program for New Century Excellent Talents in University (NCET-12-0603).

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Authors and Affiliations

  1. College of Environmental and Energy Engineering, Beijing University of Technology, Beijing, 100124, People’s Republic of China

    Zhihui Yu, Yanna Kou, Ying Dai & Xiayan Wang

  2. College of Engineering, Peking University, Beijing, 100871, People’s Republic of China

    Hang Wei & Dingguo Xia

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  1. Zhihui Yu
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  2. Yanna Kou
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  3. Ying Dai
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  4. Xiayan Wang
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  5. Hang Wei
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  6. Dingguo Xia
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Correspondence to Dingguo Xia.

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Yu, Z., Kou, Y., Dai, Y. et al. Direct Electrochemistry of Glucose Oxidase on a Three-Dimensional Porous Zirconium Phosphate–Carbon Aerogel Composite. Electrocatalysis 6, 341–347 (2015). https://doi.org/10.1007/s12678-015-0249-y

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