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Direct electrochemistry of GOD on nitrogen-doped porous carbon and its biosensing

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Abstract

Nitrogen-doped porous carbon (N-DPC) was prepared via a simple and effective method and was characterized by X-ray photoelectron spectroscopy, energy dispersive X-ray spectroscopy, N2 adsorption–desorption isotherms and scanning electron microscopy. The results showed that the N-DPC with two type reticular porosities in an average diameter of 10–100 nm has a large specific surface area, which is favorable to immobilize the redox proteins for constructing biosensors. Direct electrochemistry of glucose oxidase (GOD) on the N-DPC-modified electrode was investigated. UV–vis spectroscopy showed that GOD retained its catalytic activity in the N-DPC film. Electrochemical results indicated that the modified electrode exhibited effective direct electron transfer. It demonstrated that such N-DPC could provide a good matrix for direct electrochemistry of enzymes. A novel biosensor was developed by entrapping GOD in the N-DPC-modified electrode for glucose detection and showed a stable, rapid, and reproducible electrocatalytic response, a high sensitivity, a wide linear range and a low detection limit. Moreover, the biosensor can be applied in practical analysis and exhibit good reproducibility and long-term stability.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (21165010, 21465014, and 21465015), Young Scientist Foundation of Jiangxi Province (20112BCB23006), Foundation of Jiangxi Educational Committee (GJJ13243), the Open Project Program of State Key Laboratory of Electroanalytical Chemistry (SKLEAC201310), the Open Project Program of Key Laboratory of Functional Small organic molecule, Ministry of Education, Jiangxi Normal University (No. KLFS-KF-201214; KLFS-KF-201218).

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Authors and Affiliations

  1. Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, China

    Min Sun, Hongyu Liu, Shouhui Chen, Yonghai Song & Li Wang

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  1. Min Sun
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  2. Hongyu Liu
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  3. Shouhui Chen
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  4. Yonghai Song
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  5. Li Wang
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Correspondence to Li Wang.

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Sun, M., Liu, H., Chen, S. et al. Direct electrochemistry of GOD on nitrogen-doped porous carbon and its biosensing. J Nanopart Res 16, 2707 (2014). https://doi.org/10.1007/s11051-014-2707-5

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