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Fabrication of Nontoxic Dye-Embedded Silica Particles for Live Cell Imaging Purposes

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Abstract

Fluorescent imaging agents are desirable components for biological imaging applications. Herein, silica particles with strong fluorescent properties have been prepared by Stöber process for live cell imaging purposes. The morphological and chemical properties of synthesized dye-embedded silica particles were characterized by field emission scanning electron microscopy, energy dispersive X-ray, and X-ray photoelectron spectroscopy. Photoluminescence spectroscopy was used to investigate the room temperature fluorescence excitation and emission spectra of synthesized silica particles. It was found that this kind of fluorescent silica particles exhibits a good biocompatibility and a cellular uptake of the probes verified that they maintain well their optical properties within the intracellular environment.

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Acknowledgments

This work was supported by Seoul National University R&D research grant (no. 3348—20110053).

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Authors and Affiliations

  1. Department of Physics and Astronomy, Seoul National University, Seoul, 151-747, Republic of Korea

    Timur Sh. Atabaev & Nguyen Hoa Hong

  2. Department of Biotechnology, National University of Uzbekistan, Tashkent, 100714, Republic of Uzbekistan

    Gulnoza Urmanova

  3. Institute of Physics and Electronics, University of Peshawar, 2500, Khyber Pakhtunkhwa, Peshawar, Pakistan

    Muhammad Ajmal

Authors
  1. Timur Sh. Atabaev
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  2. Gulnoza Urmanova
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  3. Muhammad Ajmal
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  4. Nguyen Hoa Hong
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Correspondence to Timur Sh. Atabaev or Nguyen Hoa Hong.

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Atabaev, T.S., Urmanova, G., Ajmal, M. et al. Fabrication of Nontoxic Dye-Embedded Silica Particles for Live Cell Imaging Purposes. BioNanoSci. 3, 132–136 (2013). https://doi.org/10.1007/s12668-013-0082-9

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  • DOI: https://doi.org/10.1007/s12668-013-0082-9

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