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Decolorization of Congo red mediated by marine Alcaligenes species isolated from Indian West coast sediments

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Abstract

Alcaligenes species capable of degrading highly recalcitrant, carcinogenic, water-soluble dye—Congo red—were isolated from Indian West coastal sediments. Individual strains showed decolorization rates ranging from 76.49 to 98.76% within 24–48 h. Decolorization was most efficient at anoxic conditions catalyzed by intracellular azoreductase enzyme with an activity of 0.032 µmol min−1 mg−1 of protein. Degradation was confirmed by HPLC and FTIR analysis. LC/MS analysis of degraded metabolites established the cleavage of the azo bond-producing biphenyl diamine and 1,2′-diaminonapthalene-4-sulfonic acid. These results signify the effectiveness and ease to engineer processes such as feed batch/immobilized cell systems using these strains as biocatalysts to address the problem of global coastal water pollution caused by increased disposal of azo dye-containing industrial effluents.

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Acknowledgements

The authors are grateful to Director CSIR-National Institute of Oceanography Goa and Scientist-in-charge RC—Mumbai—for providing the infrastructure facilities and financial support. The authors are also thankful to Dr. Supriya Shet Tilvi for FTIR analysis. The funding for this work was provided by Ministry of Earth Sciences (MOES), Government of India. This is the CSIR-NIO contribution number 6121.

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  1. Abhay B. Fulke

    Present address: CSIR- National Institute of Oceanography (CSIR-NIO), Regional Centre, Lokhandwala Road, Four Bungalows, Andheri (West), Mumbai, 400053, India

Authors and Affiliations

  1. CSIR- National Institute of Oceanography (CSIR-NIO), Regional Centre, Lokhandwala Road, Four Bungalows, Andheri (West), Mumbai, 400053, India

    Edna D’Souza, Najmuddin Mulani, Anirudh Ram, Manish Asodekar & S. N. Gajbhiye

  2. CSIR- Indian Institute of Integrative Medicine (CSIR-IIIM), CSIR Innovation Complex, Mumbai, Maharashtra, 400053, India

    Niteen Narkhede

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  1. Edna D’Souza
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  2. Abhay B. Fulke
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  3. Najmuddin Mulani
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Correspondence to Abhay B. Fulke.

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D’Souza, E., Fulke, A.B., Mulani, N. et al. Decolorization of Congo red mediated by marine Alcaligenes species isolated from Indian West coast sediments. Environ Earth Sci 76, 721 (2017). https://doi.org/10.1007/s12665-017-7077-8

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