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Environmental Earth Sciences

, Volume 71, Issue 5, pp 2177–2183 | Cite as

Effect of metal content on chlorophyll fluorescence and chlorophyll degradation in lichen Pyxine cocoes (Sw.) Nyl.: a case study from Uttar Pradesh, India

  • Neha Karakoti
  • Rajesh Bajpai
  • D. K. UpretiEmail author
  • G. K. Mishra
  • A. Srivastava
  • S. Nayaka
Original Article

Abstract

The major aim of the present study is to identify the relationship of physiological parameters of the photosynthetic system with the elemental content of the naturally growing lichen Pyxine cocoes. The epiphytic foliose lichen P. cocoes was used as biomonitoring indicator and effect of atmospheric pollutants on physiological integrity was examined. Potential quantum yield of Photosystem II (fluorescence ratio Fv/Fm), chlorophyll degradation ratio and quantitative estimation of Al, As, Cd, Cr, Cu, Pb, Fe and Zn contained in the lichen thallus were ascertained. Statistical analysis revealed significantly positive correlations between Fv/Fm and element contents (Al and Cr). The chlorophyll degradation as well as alteration in the pigment content was found to be the most sensitive parameters to assess the vitality of lichen thallus against polluted environment. The species accumulated higher amounts of elements (Al, As, Cu, Fe and Zn) in the polluted sites as compared to the non polluted sites. It was also evident from this study that vehicular emission played a significant role in the release of elements as pollutants in the surrounding environment. The effectiveness of this lichen could be further investigated by comparing this species with other biomonitors.

Keywords

Chlorophyll fluorescence Lichens Elements Photosystem II 

Notes

Acknowledgments

The authors are grateful to Dr. C. S. Nautiyal, Director, CSIR-National Botanical Research Institute Lucknow, India for providing laboratory facilities to work. We are also thankful to U. P. Biodiversity Board, Uttar Pradesh. One of the authors (RB) is grateful to SERB Division, Department of Science and Technology (DST-SERB), New Delhi for Young Scientist Fellow (SR/FTP/ES-134/2010).

References

  1. Adriano DC (2001) Trace elements in terrestrial environment, 2nd edn. Springer-Verlag, New YorkCrossRefGoogle Scholar
  2. Bajpai R, Upreti DK, Nayaka S, Kumari B (2010) Biodiversity, bioaccumulation and physiological changes in lichens growing in the vicinity of coal-based thermal power plant of Raebareily district, north India. J Hazard Mat 174:429–436CrossRefGoogle Scholar
  3. Bajpai R, Pandey AK, Deeba F, Upreti DK, Nayaka S, Pandey V (2012) Physiological effects of arsenate on transplant thalli of the lichen Pyxine cocoes (Sw.) Nyl. Environ Sci Pollut Res 19:1494–1502CrossRefGoogle Scholar
  4. Fang GC, Huang CS (2012) Monitoring and modeling concentration and dry deposition of ambient air particulates and metallic elements Mn, Fe, Zn, Cr and Cu in central Taiwan. Environ Earth Sci. doi: 10.1007/s12665-012-1960-0 (online)Google Scholar
  5. Garty J (2001) Biomonitoring atmospheric heavy metals with lichens: theory and application. Crit Rev Plant Sci 20(4):309–371CrossRefGoogle Scholar
  6. Garty J, Weissman L, Tamir O, Beer S, Cohen Y, Karnidi A, Orlovsky L (2000) Comparison of five physiological parameters to assess the vitality of lichen Ramalina lacera exposed to air pollution. Physiol Plant 9:410–518CrossRefGoogle Scholar
  7. Gries C, Sanz MJ, Nash TH III (1995) The effect of SO2 fumigation on CO2 gas exchange, chlorophyll fluorescence and chlorophyll degradation in different lichen species from western North America. Crypt Bot 5:239–246Google Scholar
  8. Jensen M, Kricke R (2002) Chlorophyll fluorescence measurements in the field: assessment of the vitality of large number of lichen thalli. In: Nimis PL, Scheidegger C, Wolseley PA (eds) Monitoring with lichens—monitoring lichens. Kluwer Academic Publisher, The Netherlands pp, pp 327–332CrossRefGoogle Scholar
  9. Kim Y, Kim BK, Kim K (2010) Distribution and speciation of heavy metals and their sources in Kumho River sediment, Korea. Environ Earth Sci 60:943–952CrossRefGoogle Scholar
  10. Loppi S, Cenni E, Bussot E, Ferreti M (1998) Biomonitoring of geothermal air pollution by epiphytic lichens and forest trees. Chemo 36:1079–1082CrossRefGoogle Scholar
  11. Loppi S, Putorti E, Pirintsos SA, Dominicis VD (2000) Accumulation of heavy metals in epiphytic lichens near municipal waste incinerator (central Italy). Environ Monit Assess 61:361–371CrossRefGoogle Scholar
  12. Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668CrossRefGoogle Scholar
  13. Moran R (1982) Formulae for determination of chlorophyllous pigments extracted with N-dimethyl formamide. Plant Physiol 69:1376–1381CrossRefGoogle Scholar
  14. Mrak T, Šlejkovec Z, Jeran Z (2006) Extraction of arsenic compounds from lichens. Talanta 69:251–258CrossRefGoogle Scholar
  15. Nimis PL, Castello M, Perotti M (2002) Lichens as bioindicators of heavy metal pollution: A case study at La Spezia (N. Italy), the environment. In: Markert B (ed) Plants as biomonitors, indicators for VCH Germany. International Conference, September 1989. vol. II. Geneva, CEP, pp 265–284Google Scholar
  16. Richardson DHS (1992) Pollution monitoring with lichens. Richmond Publishing, SloughGoogle Scholar
  17. Ronen R, Galun M (1984) Pigment extraction from lichens with dimethyl sulfoxide (DMSO) and estimation of chlorophyll degradation. Environ Exp Bot 24:239–245CrossRefGoogle Scholar
  18. Schieleit P, Ott S (1994) Ethylene production in lichens with respect to possible bacterial contamination. Lichenologist 29:492–495CrossRefGoogle Scholar
  19. Shukla V, Upreti DK (2012) Air quality monitoring with lichens in India: Heavy metals and Polycyclic aromatic hydrocarbon. In: Lichtfouse E, Schwarzbauer J, Robert D (eds) Environmental Chemistry for a sustainable world, vol 2., Remediation of air and water pollutionSpringer Verlag, New York, pp 277–294CrossRefGoogle Scholar
  20. Wang XS (2013) Heavy metal pollution in urban top soil: mineralogical analysis and magnetic characterzation. Environ Earth Sci. doi: 10.1007/s12665-013-2380-5 (Online)Google Scholar
  21. Ward NI, Sampson KE (1989) The use of bryophytes to monitor the rate of metal deposition along the London orbital (M25) motorway. In: Vernet JP (ed) Heavy metals in the environment. Proceedings of the International Conference, September 1989, CEP Consultants, Vol. 2. Geneva, Edinburgh, UK, pp 444–447Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Neha Karakoti
    • 1
  • Rajesh Bajpai
    • 1
  • D. K. Upreti
    • 1
    Email author
  • G. K. Mishra
    • 1
  • A. Srivastava
    • 1
  • S. Nayaka
    • 1
  1. 1.Lichenology Laboratory, Plant Diversity Systematics and Herbarium DivisionCSIR-National Botanical Research InstituteLucknowIndia

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