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Impacts of nitrogen and phosphorus on atrazine-contaminated soil remediation and detoxification by Arthrobacter sp. strain HB-5

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Abstract

The relationship of atrazine-degrading bacteria Arthrobacter sp. HB-5 and nitrogen and phosphorus fertilizer to atrazine degradation and detoxification in soil was investigated in a microcosm pot experiment. Treatments of soil containing atrazine (AW) with atrazine plus strain HB-5 alone (A), together with atrazine and strain HB-5 plus nitrogen alone (AN), phosphate alone (AP), and nitrogen and phosphate together (ANP) were used to investigate atrazine degradation and ecotoxicity. Atrazine residues in the soils were determined by high performance liquid chromatography, while soil ecotoxicity was tested by micronucleus (MN) assay of Vicia faba root tip cells. The results showed that degradation of atrazine in soil could be facilitated by the treatment of strain HB-5 as well as strain HB-5 application with the addition of nitrogenous and/or phosphorus fertilizers. The degradation rates varied as the following: ANP > AP > AN > A > AW in different treatments. At 10 days post treatment, degradation efficiency of over 90 % was achieved in all strain HB-5 treatments except AW, but with no statistically significant differences found between treatments. Soil ecotoxicity was significantly reduced along with the degradation of atrazine by strain HB-5, and the ecotoxicity of soils with applied fertilizer was below that of treatments without fertilizer. On the seventh day and later, the MN frequencies of all treatments were decreased in the control levels except for AW. Thus, adjusting soil nutrient contents not only promoted strain HB-5 to remove atrazine in soil but also mitigated soil ecotoxicity effects caused by atrazine. These results are important keystones for future remediation of atrazine-contaminated soils.

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Acknowledgments

The authors thank the two anonymous reviewers for their critical comments on the manuscript. This study was funded by the National Natural Science Foundation of China [No.41071164, 40801203, 41001152 and 21277083] and the Specialized Research Fund for the Doctoral Program of Higher Education (20113702110007).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, 61 Daizong Road, Taian, 271018, China

    Qingming Zhang, Lusheng Zhu, Jun Su, Jun Wang, Hui Xie, Jinhua Wang & Fenghua Wang

  2. College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, China

    Qingming Zhang

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  1. Qingming Zhang
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  2. Lusheng Zhu
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  3. Jun Su
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Correspondence to Lusheng Zhu.

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Zhang, Q., Zhu, L., Su, J. et al. Impacts of nitrogen and phosphorus on atrazine-contaminated soil remediation and detoxification by Arthrobacter sp. strain HB-5. Environ Earth Sci 71, 1465–1471 (2014). https://doi.org/10.1007/s12665-013-2551-4

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