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Bioactivity Screening of Hydrolysates From Brown Crab Processing Side Streams Fermented by Marine Pseudoalteromonas Strains

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Abstract

Purpose

This study aims to employ five marine Pseudoalteromonas strains, belonging to Psa. arctica DSM 18437T, Psa. carrageenovora DSM 6820T, Psa. issachenkonii LMG 19697T, Psa. rubra DSM 6842T and Psa. tunicata DSM 14096T to ferment brown crab processing side streams.

Methods

The generated hydrolysates after the fermentation were screened for their antioxidant, anthelmintic and anti-biofilm activities.

Results

After 72 h, the highest degree of hydrolysis (DoH) of 14.92% was reached by Psa. issachenkonii. The highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability was obtained by hydrolysates of Psa. rubra with an IC50 of 2.23 mg/mL while for the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging ability, the maximum was reached by the hydrolysates of Psa. tunicata with an IC50 of 1.23 mg/mL. Anthelmintic activity, as tested on Caenorhabditis elegans, revealed a 99% mortality rate at 1 mg/mL with ethyl acetate extracts of the hydrolysates of Psa. arctica, Psa. issachenkonii and Psa. rubra. In terms of antibiofilm activity, aqueous extracts of the hydrolysates of Psa. arctica and Psa. issachenkonii at 500 µg/mL could dramatically decrease the biofilm formation index of Staphylococcus epidermidis RP62A from 1.35 to 0.37 and 0.36, respectively.

Conclusions

Brown crab processing side streams could be valorized by marine Pseudoalteromonas bacteria fermentation for the production of a mixture of bioactive compounds.

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Acknowledgements

This study was performed in the framework of the BlueShell project (Exploring Shellfish By-Products as a source of Blue Bioactivies) supported by ERA-Marine Biotech (ERAMBT) and Fonds voor Wetenschappelijk Onderzoek— Vlaanderen (FWO).

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Authors and Affiliations

  1. Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University Campus Kortrijk, Graaf Karel de Goedelaan 5, 8500, Kortrijk, Belgium

    Yang Zou & Katleen Raes

  2. Flanders Research Institute for Agriculture, Fisheries and Food, Burgemeester Van Gansberghelaan 92 bus 1, 9820, Merelbeke, Belgium

    Yang Zou, Johan Robbens, Marc Heyndrickx & Jane Debode

  3. Institute of Biochemistry and Cellular Biology, National Research Council, Via Pietro Castellino 111, 80121, Naples, Italy

    Emiliana Tortorella & Donatella De Pascale

  4. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University Campus Merelbeke, Salisburylaan 133, 9820, Merelbeke, Belgium

    Marc Heyndrickx

  5. Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Naples, Italy

    Donatella De Pascale

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  1. Yang Zou
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Zou, Y., Tortorella, E., Robbens, J. et al. Bioactivity Screening of Hydrolysates From Brown Crab Processing Side Streams Fermented by Marine Pseudoalteromonas Strains. Waste Biomass Valor 12, 2459–2468 (2021). https://doi.org/10.1007/s12649-020-01195-y

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