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Anti-Biofilm, Antioxidant and Cytotoxic Potential of F5, a Peptide Derived from Waste Generated During the Processing of the White Shrimp, Metapenaeus monoceros (Fabricius, 1798)

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Abstract

In previous research works, we have described the production of 7 kDa F5 peptide after a hydrolysis of the white shrimp, Metapenaeus monoceros (Fabricius, 1798) by-product, using a serine alkaline protease (SPVP) purified from Aeribacillus pallidus strain VP3. The present study aims to explore the antioxidative potentials of F5 peptide by both in vitro and in vivo assays. The anti-biofilm activity was performed, showing 50% inhibition at 2 µg/mL, 3 µg/mL, 19 µg/mL, and 45 µg/mL for Staphylococcus aureus, Escherichia coli, Bacillus cereus, and Pseudomonas aeruginosa, respectively. Consistently, the antioxidative capacity was tested in vitro against 2,2′-azino-bis-(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) cation radical, β-caroten-linoleic acid bleaching, chelating capacity of ferrous ion, and ferric reducing power assays. The cytotoxic effects of F5 peptide on the Human embryonic kidney HEK293 cells were subsequently tested. Remarkably, the F5 peptide was able to improve significantly HEK293 cell viability. To further assess this bioactivity, an in vivo study was performed on adult mice models. The animals were divided into three groups: controls, 100 mg and 200 mg of F5/kg per bodyweight. Indeed, the F5 peptide could prevent the lipid and protein oxidation damage in kidney cells, improving the antioxidative enzymatic and non-enzymatic capacities.

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Data Availability

The authors declare that all datasets supporting the findings, used, and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank Prof. M. Chamkha (LBPE, CBS, Tunisia) for his kind gift of the Aeribacillus pallidus strain VP3. They also extend their thanks to Mr. C. Tmar (Dept. Biology, Faculty of Sciences, University of Sfax, Tunisia) for his kind assistance in feeding and watering mice in the Pet Shop during the experimentation period. The authors would also like to express their sincere gratitude to Mrs. L. Mahfoudhi, Professor of English language, Senior ESL/EFL Instructor, Translator, and Proofreader expert, from the English Language Section of the Faculty of Sciences of Sfax, University of Sfax, Tunisia, for English editing and proofreading services.

Funding

This study was supported by the Ministry of Higher Education and Scientific Research (MESRS) in Tunisia under the framework of the Contract Programs LBMIE-CBS, code Grants Nos.: LR15CBS06 (2015–2018 and 2019–2022), the Multilateral Project Partenariats Hubert Curien (PHC)-Maghreb 2020 Program (FranMaghZYM 2020–2023, code Campus France: 43791TM & code PHC: 01MAG20), and the Algerian-Tunisian RDP Cooperation Program 2021–2024 (AliPoiAgro, code PRD/TN/DZ/21/13).

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Authors and Affiliations

  1. Laboratory of Microbial Biotechnologyand Engineering Enzymes, Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia

    Sondes Mechri, Fadoua Jabeur, Wacim Bejar & Bassem Jaouadi

  2. Laboratory of Enzyme Engineering and Microbiology, Engineering National School of Sfax, University of Sfax, Road of Soukra Km 3.5, P.O. Box 1173, 3038, Sfax, Tunisia

    Imen Jaballi & Mohamed Hajji

  3. Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, University of Monastir, Road of Ibn Sina, P.O. Box 56, 5000, Monastir, Tunisia

    Fadia Ben Taheur, Chalbia Mansour, Ridha Mzoughi & Kamel Chaieb

  4. Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia

    Jihen Elloumi

  5. Laboratory of Toxicology Microbiology, and Environmental Health, Faculty of Sciences, University of Sfax, Road of Soukra Km 4, P.O. Box 1171, 3000, Sfax, Tunisia

    Hamadi Fetoui

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  1. Sondes Mechri
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Correspondence to Bassem Jaouadi.

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Ethical Approval

The experimental proceedings were executed following the general recommendations on the use of living animals in scientific explorations, endorsed by the Ethical Committee of the Sciences Faculty of Guidelines for Care and Use of Laboratory Animals of Tunis University and approved by the Animal Ethics Committee of National Institute of Health Sfax. These efforts were followed to reduce animal numbers and their sufferance.

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Mechri, S., Jaballi, I., Ben Taheur, F. et al. Anti-Biofilm, Antioxidant and Cytotoxic Potential of F5, a Peptide Derived from Waste Generated During the Processing of the White Shrimp, Metapenaeus monoceros (Fabricius, 1798). Waste Biomass Valor 13, 3233–3244 (2022). https://doi.org/10.1007/s12649-022-01722-z

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