Skip to main content
Log in

3rd Generation Bioethanol Production from Microalgae Isolated from Slaughterhouse Wastewater

  • Original Paper
  • Published:
Waste and Biomass Valorization Aims and scope Submit manuscript

Abstract

The present study consists of an essay about the 3rd generation bioethanol production from microalgae. The strain of microalgae used in this research has been isolated from slaughterhouse wastewater. The study starts by applying five methods (thermic shock, grinding, acid treatment, basic treatment, and enzyme hydrolysis) to disrupt cell wall. The maximum total sugar content has been obtained after enzymatic hydrolysis with a yield of 82.2 % relative to dry matter. The fermentation was applied on the recovered supernatant after pH setting (7 ± 0.1) in presence of the yeast Saccharomyces cereviseae. The maximum of ethanol production was obtained with basic, enzymatic and acid treatments with a yield of 43, 40 and 38 % respectively. These results demonstrate the importance of the microalgae strain used as an attractive feedstock for third generation bioethanol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Shilton, A., Guieyesse, B.: Sustainable sunlight to biogas is via marginal organics. Curr. Opin. Biotechnol. 21, 287–291 (2010)

    Article  Google Scholar 

  2. Rawat, I., Ranjith Kumar, R., Mutanda, T., Bux, F.: Biodiesel from microalgae: a critical evaluation from laboratory to large scale production. Appl. Energy 103, 444–467 (2013)

    Article  Google Scholar 

  3. Khambhaty, Y., Mody, K., Gandhi, M.R., Thampy, S., Maiti, P., Brahmbhatt, H., Eswaran, K., Ghosh, P.K.: Kappaphycus alvarezii as a source of bioethanol. Bioresour. Technol. 103, 180–185 (2012)

    Article  Google Scholar 

  4. Fargione, J., Hill, J., Tilman, D., Polasky, S., Hawthorne, P.: Land clearing and the biofuel carbon debt. Science 319, 1235–1238 (2008)

    Article  Google Scholar 

  5. Daroch, M., Mos, M.: Role of biotechnology in developing the bioenergy sector in Poland. BioTechnologia. 92, 23–32 (2011)

    Article  Google Scholar 

  6. Molina-Grima, E., Acién-Fernández, F.G., Robles-Medina, A.: Downstream processing of cell-mass and products. In: Richmond, A. (ed.) Handbook of Microalgal Culture: Biotechnology and Applied Phycology, pp. 215–252. Blackwell, London (2004)

    Google Scholar 

  7. Nguyen, M.T., Choi, S.P., Lee, J., Lee, J.H., Sim, S.J.: Hydrothermal acid pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. J. Microbiol. Biotechnol. 19, 161–166 (2009)

    Article  Google Scholar 

  8. Fu, C.C., Hung, T.-C., Chen, J.-Y., Su, C.-H., Wu, W.-T.: Hydrolysis of microalgae cell walls for production of reducing sugar and lipid extraction. Bioresour. Technol. 101, 8750–8754 (2010)

    Article  Google Scholar 

  9. Harun, R., Jason, W.S.Y., Cherrington, T., Danquah, M.K.: Exploring alkaline pre-treatment of microalgal biomass for bioethanol production. Appl. Energy 88, 3464–3467 (2011)

    Article  Google Scholar 

  10. Harun, R., Danquah, M.K.: Influence of acid pre-treatment on microalgal biomass for bioethanol production. Process Biochem. 46, 304–309 (2011)

    Article  Google Scholar 

  11. Alvira, P., Tomas-Peja, E., Ballesteros, M., Negro, M.J.: Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour. Technol. 101, 4851–4861 (2009)

    Article  Google Scholar 

  12. Eshaq, F.S., Ali, M.N., Mohd, M.K.: Spirogyra biomass a renewable source for biofuel (bioethanol) production. Eng. Sci. Technol. 2(12), 7045–7054 (2010)

    Google Scholar 

  13. Yang, B., Dai, Z., Ding, S.-Y., Wyman, C.E.: Enzymatic hydrolysis of cellulosic biomass. Biofuels. 2(4), 421–450 (2011)

    Article  Google Scholar 

  14. Chu, F.L.E., Dupuy, J.L., Webb, K.L.: Polysaccharide composition of five algal species used as food for larvae of the American oyster, Crassostrea virginica. Aquaculture 29, 241–252 (1982)

    Article  Google Scholar 

  15. Liu, J., Pan, Y., Yao, Ch., Wang, H., Cao, X., Xue, S.: Determination of ash content and concomitant acquisition of cell compositions in microalgae via thermogravimetric (TG) analysis. Algal Research. 12, 149–155 (2015)

    Article  Google Scholar 

  16. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination on sugars and related substances. Anal. Chem. 28, 350–356 (1956)

    Article  Google Scholar 

  17. Miller, G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426–428 (1959)

    Article  Google Scholar 

  18. Řezanka, T., Dembitsky, V.M.: Metabolites produced by cyanobacteria belonging to several species of the family Nostocaceae. Folia Microbiol. 51(3), 159–182 (2006)

    Article  Google Scholar 

  19. Spolaore, P., Joannis-Cassan, C., Duran, E., Isambert, A.: Commercial applications of microalgae. J. Biosci. Bioeng. 101(2), 87–96 (2006)

    Article  Google Scholar 

  20. Becker, W.: Microalgae in human and animal nutrition. In: Richmond, A. (ed.) Handbook of Microalgal Culture. Blackwell, Oxford (2004)

    Google Scholar 

  21. Chacón-Lee, T.L., González-Mariño, G.E.: Microalgae for “Healthy” foods—possibilities and challenges. Compr. Rev. Food Sci. Food Saf. 9(6), 655–675 (2010)

    Article  Google Scholar 

  22. Brown, M.R., Jeffrey, S.W.: Biochemical composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 1. Amino acids, sugars and pigments. J. Exp. Mar. Biol. Ecol. 161(1), 91–113 (1992)

    Article  Google Scholar 

  23. Harun, R., Danquah, M.K.: Enzymatic hydrolysis of microalgal biomass for bioethanol production. Chem. Eng. J. 168, 1079–1084 (2011)

    Article  Google Scholar 

  24. Demirbas, M.F.: Biofuels from algae for sustainable development. Appl. Energy 88, 3473–3480 (2011)

    Article  Google Scholar 

  25. Li, K., Liu, S., Liu, X.: An overview of algae bioethanol production. Int. J. Energy Res. 38, 965–977 (2014)

    Article  Google Scholar 

  26. Percival E., McDowell R. H.: Algal walls. Composition and biosynthesis. In Encyclopedia of Plant Physiology, Vol. 13B, Tanner W., Loewus F. A., editors. (Berlin: Springer) pp 277–316 (1981)

  27. Estevez, J.M., Leonardi, P.I., Alberghina, J.S.: Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae F. minor (Oedogoniales, Chlorophyta). J. Phycol. 44, 1257–1268 (2008)

    Article  Google Scholar 

  28. Sørensen, I., Rose, J.K.C., Doyle, J.J., Domozych, D.S., Willats, W.G.T.: The Charophycean green algae as model systems to study plant cell walls and other evolutionary adaptations that gave rise to land plant. Plant Signal. Behav. 7, 1–3 (2012). doi:10.4161/psb.7.1.18574

    Article  Google Scholar 

  29. Ho, S.H., Huang, S.W., Chen, C.Y., Hasunuma, T., Kondo, A., Chang, J.S.: Bioethanol production using carbohydrate-rich microalgae biomass as feedstock. Bioresour. Technol. 135, 191–198 (2013)

    Article  Google Scholar 

  30. Rabelo, S.C., Maciel-Filho, R., Costa, A.C.: Lime pretreatment of sugarcane bagasse for bioethanol production. Appl. Biochem. Biotechnol. 153, 139–150 (2009). doi:10.1007/s12010-008-8433-7

    Article  Google Scholar 

  31. Choi, S.P., Nguyen, M.T., Sim, S.J.: Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. Bioresour. Technol. 101(14), 5330–5336 (2010)

    Article  Google Scholar 

  32. Guo, H., Daroch, M., Liu, L., Qiu, G., Geng, S., Wang, G.: Biochemical features and bioethanol production of microalgae from coastal waters of Pearl River Delta. Bioresour. Technol. 127, 422–428 (2013)

    Article  Google Scholar 

  33. Harun, R., Singh, M., Forde, G.M., Danquah, M.K.: Bioprocess engineering of microalgae to produce a variety of consumer products. Renew. Sustain. Energy Rev. 14, 1037–1047 (2010)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to F. Saïdane-Bchir.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saïdane-Bchir, F., El Falleh, A., Ghabbarou, E. et al. 3rd Generation Bioethanol Production from Microalgae Isolated from Slaughterhouse Wastewater. Waste Biomass Valor 7, 1041–1046 (2016). https://doi.org/10.1007/s12649-016-9492-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12649-016-9492-6

Keywords

Navigation