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Antioxidant Activity and Total Phenolic Content of Oils Extracted from Pinus pinaster Sawdust Waste. Screening of Different Innovative Isolation Techniques

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Abstract

Essential oils from Pinus pinaster sawdust waste was extracted using different technologies including conventional hyrodistillation method (HD) and some intensified technologies namely turbo-hydrodistillation (THD), ultrasound assisted extraction HD (UAE-HD) and two microwave techniques; microwave hydrodiffusion and gravity (MHG) and solvent-free microwave extraction (SFME). The essential oils were qualified and quantified using chromatography coupled to mass spectrometry analysis. The analysis of the oil has resulted in the identification of 45 components; α-terpineol and β-caryophyllene were the main components. The antioxidant activity was assessed by diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power (FRAP) assays. The highest radical-scavenging activity was obtained for the two microwave techniques with IC50 value about 15 μg/ml, followed by the other techniques for which IC50 ranged between 59.8 and 123 μg/ml. For FRAP assay, the same classification was obtained with the highest values of 0.598 and 0.591 μg/ml for respectively microwave extractions (MHG and SFME) and 0.307, 0.322 and 0.401 μg/ml respectively for HD, THD and UAE-HD. DPPH and FRAP assays were found to be highly correlated. The total phenolic compounds (TPC) were more present in microwave extracts (78.5 and 74.6 GAE/g extract) respectively for MHG and SFME) compared to other technique for which the values ranger between 54.1 and 56.6 GAE/g extract. TPC were correlated with DPPH and FRAP (R2 > 0.95; p < 0.01) indicating that the total phenols are highly contributes to the antioxydant activity.

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References

  1. Imelouane, B., Amhamdi, H., Wathelet, J.P., Ankit, M., Khedid, K., El Bachiri, A.: Chemical composition and antimicrobial activity of essential oil of thyme (Thymus vulgaris) from eastern morocco. Int. J. Agric. Biol. 11, 205–208 (2009)

    Google Scholar 

  2. Pistelli, L., Mancianti, F., Bertoli, A., Cioni, P.L., Leonardi, M., Pisseri, F., Mugnaini, L., Nardoni, S.: Antimycotic activity of some aromatic plants essential oils against canine isolates of Malassezia pachydermatis: an in vitro assay. Open Mycol. J. 6, 17–21 (2012)

    Article  Google Scholar 

  3. Maimoona, A., Naeem, I., Saddiqe, Z., Jameel, K.: A review on biological, nutraceutical and clinical aspects of French maritime pine bark extract. J. Ethnopharmacol. 133, 261–277 (2011)

    Article  Google Scholar 

  4. Ozkan, A., Erdogan, A.: A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turk. J. Biol. 35, 735–742 (2011)

    Google Scholar 

  5. Hossain, M.A., Shah, M.D.. A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arab. J Chem. (in Press). doi:10.1016/j.arabjc.2011.01.007

  6. Dorman, H.J.D., Surai, P., Deans, S.G.: In vitro antioxidant activity of a number of plant essential oils and phytoconstituents. J. Essent. Oil Res. 12, 241–248 (2000)

    Google Scholar 

  7. Chen, X.N., Fan, J.F., Yue, X., Wu, X.R., Li, L.T.: Radical scavenging activity and phenolic compounds in persimmon (Diospyros kaki L. cv. Mopan). J. Food Sci. 73, 24–28 (2008)

    Article  Google Scholar 

  8. Vázquez, G., Freire, M.S., Santos, J., Antorrena, G., González-Álvarez, J.: Optimisation of polyphenols extraction from chestnut shell by response surface methodology. Waste Biomass Valor. 1, 219–225 (2010)

    Article  Google Scholar 

  9. Losso, J.N., Shahidi, F., Bagchi, D.: Anti-Angiogenic Functional and Medicinal Foods. Taylor & Francis, Boca Raton, FL (2007)

    Book  Google Scholar 

  10. Sarikurkcu, C., Tepe, B., Daferera, D., Polissiou, M., Harmandar, M.: Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. Globusum (lamiaceae) by three chemical assays. Bioresour. Technol. 99, 4239–4246 (2007)

    Article  Google Scholar 

  11. Li, H.B., Wong, C.C., Cheng, K.W., Chen, F.: Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT-Food Sci. Technol. 41, 385–390 (2008)

    Article  Google Scholar 

  12. Tepe, B., Sokmen, M., Akpulat, H.A., Daferera, D., Polissiou, M., Sokmen, A.: Antioxidative activity of the essential oils of Thymus sipyleus subsp. sipyleus var. sipyleus and Thymus sipyleus subsp. Sipyleus var. rosulans. J. Food Eng. 66, 447–454 (2005)

    Article  Google Scholar 

  13. Donelian, A., Carlson, L.H.C., Lopes, T.J., Machado, R.A.F.: Comparison of extraction of patchouli (Pogostemon cablin) essential oil with supercritical CO2 and by steam distillation. J. Supercrit. Fluid 48, 15–20 (2009)

    Google Scholar 

  14. Ma, C.-H., Yang, L., Zu, Y.-G.: Liu, T-T. Optimization of conditions of solvent-free microwave extraction and study on antioxidant capacity of essential oil from Schisandra chinensis (Turcz.) Baill. Food Chem. 134, 2532–2539 (2012)

    Article  Google Scholar 

  15. Suanarunsawat, T., Na Ayutthaya, W.D., Songsak, T., Thirawarapan, S., Poungshompoo, S.: Antioxidant activity and lipid-lowering effect of essential oils extracted from ocimum sanctum L. leaves in rats fed with a high cholesterol diet. J. Clin. Biochem. Nutr. 46, 52–59 (2010)

    Article  Google Scholar 

  16. Zhang, X., Gao, H., Zhang, L., Liu, D., Ye, X.: Extraction of essential oil from discarded tobacco leaves by solvent extraction and steam distillation, and identification of its chemical composition. Ind. Crop. Prod. 39, 162–169 (2012)

    Article  Google Scholar 

  17. Li, X.M., Tian, S.L., Pang, Z.C., Shi, J.Y., Feng, Z.S., Zhang, Y.M.: Extraction of Cuminum cyminum essential oil by combination technology of organic solvent with low boiling point and steam distillation. Food Chem. 115, 1114–1119 (2009)

    Article  Google Scholar 

  18. Périno-Issartier, S., Zill-e-Huma, Abert-Vian, M., Chemat, F.: Solvent free microwave-assisted extraction of antioxidants from sea buckthorn (Hippophae rhamnoides) food by-products. Food Bioprocess Tech. 4, 1020–1028 (2011)

    Article  Google Scholar 

  19. Tigrine-Tordjani, N., Meklati, B.Y., Chemat, F., Guezil, F.Z.: Kinetic investigation of rosemary essential oil by two methods: solvent-free microwave extraction and hydrodistillation. Food Anal. Method 5, 596–603 (2012)

    Article  Google Scholar 

  20. Chen, Z.L., Chao, J.P., Cao, H.L., Bi, W.T., Cui, H.Y., Li, M.L.: Research on the extraction of plant volatile oils. Procedia Environ. Sci. 8, 426–432 (2011)

    Article  Google Scholar 

  21. Mhemdi, H., Rodier, E., Kechaou, N., Fages, J.: A supercritical tuneable process for the selective extraction of fats and essential oil from coriander seeds. J. Food Eng. 105, 609–616 (2011)

    Article  Google Scholar 

  22. Fornari, T., Vicente, G., Vázquez, E., García-Risco, M.R., Reglero, G.: Isolation of essential oil from different plants and herbs by supercritical fluid extraction. J. Chromatogr. A 1250, 34–48 (2012)

    Article  Google Scholar 

  23. Rezzoug, S.A., Louka, N.: Thermomechanical process intensification for oil isolation from orange peel. Innov. Food Sci. Emerg. 10, 530–536 (2009)

    Article  Google Scholar 

  24. Mellouk, H., Khezami, L., Rezzoug, S.A., Capart, R.: Total Valorisation of red cedar sawmills wastes by instantaneous controlled pressure drop process. Isolation of extractives and production of activated carbon from the solid residue. Bioresources 3, 1156–1172 (2008)

    Google Scholar 

  25. Pingret, D., Fabiano-Tixier, A.-S., Bourvellec, C.L., Renard, C.M.G.C., Chemat, F.: Lab and pilot-scale ultrasound-assisted water extraction of polyphenols from apple pomace. J. Food Eng. 111, 73–81 (2012)

    Article  Google Scholar 

  26. Shirsatha, S.R., Sonawanea, S.H., Gogate, P.R.: Intensification of extraction of natural products using ultrasonic irradiations—a review of current status. Chem. Eng. Process. 53, 10–23 (2012)

    Article  Google Scholar 

  27. Chemat, F., Zill-E-Huma, Khan, M.K.: Applications of ultrasound in food technology: processing, preservation and extraction. Ultrason. Sonochem. 18, 813–835 (2011)

    Article  Google Scholar 

  28. Ma, C.H., Liu, T.T., Yang, L., Zu, Y.G., Chen, X., Zhang, L., Zhang, Y., Zhao, C.: Ionic liquid-based microwave-assisted extraction of essential oil and biphenyl cyclooctene lignans from Schisandra chinensis Baill fruits. J. Chromatogr. A 1218, 8573–8580 (2011)

    Article  Google Scholar 

  29. Gavahian, M., Farahnaky, A., Javidnia, K., Majzoobi, M.: Comparison of ohmic-assisted hydrodistillation with traditional hydrodistillation for the extraction of essential oils from Thymus vulgaris L. Innov. Food Sci. Emerg. 14, 85–91 (2012)

    Article  Google Scholar 

  30. Jiao, J., Fu, Y.J., Zu, Y.G., Luo, M., Wang, W., Zhang, L., Li, J.: Enzyme-assisted microwave hydro-distillation essential oil from Fructus forsythia, chemical constituents, and its antimicrobial and antioxidant activities. Food Chem. 134, 235–243 (2012)

    Article  Google Scholar 

  31. European Pharmacopeia. On line version. http://online6.edqm.eu/ep705/

  32. Adams, R.P.: Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th edn. Allured Publishing Corporation, Carol Stream, IL (2007)

    Google Scholar 

  33. Lapornik, B., Prosek, M., Wondra, A.G.: Comparison of extracts prepared from plant by-products using different solvents and extraction time. J. Food Eng. 71, 214–222 (2005)

    Article  Google Scholar 

  34. Gao, H., Shupe, T.F., Eberhardt, T.L., Hse, C.Y.: Antioxidant activity of extracts from the wood and bark of Port Orford cedar. J. Wood Sci. 53, 147–152 (2007)

    Article  Google Scholar 

  35. Dorman, H.J.D., Peltoketo, A., Hiltunen, R., Tikkanen, M.J.: Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem. 83, 255–262 (2003)

    Article  Google Scholar 

  36. Benzie, I.F.F., Strain, J.J.: Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Method. Enzymol. 299, 15–27 (1999)

    Article  Google Scholar 

  37. Statgraphics Plus for Windows 5.1. Experimental Design. Manugistics, Inc. 1994

  38. Dob, T., Berramdane, T., Chelghoum, C.: Analysis of essential oil from the needles of Pinus pinaster growing in Algeria. Chem. Nat. Compd. 41, 545–548 (2005)

    Article  Google Scholar 

  39. Koukos, P.K., Papadopoulou, K.I., Patiaka, D.T., Papagiannopoulos, A.D.: Chemical composition of essential oils from needles and twigs of balkan pine (Pinus peuce Grisebach) grown in Northern Greece. J. Agric. Food Chem. 48, 1266–1268 (2000)

    Article  Google Scholar 

  40. Liu, X.G., Ju, X.R., Mao, X.D., Zhang, Z.: Studies on enzymic extraction of essential oil from pine needles. Chem. Indus. For. Prod. 25, 101–114 (2005)

    Google Scholar 

  41. Montanari, R.M., Barbosa, L.C.A., Demuner, A.J., Silva, C.J., Carvalho, L.S., Andrade, N.J.: Chemical composition and antibacterial activity of essential oils from verbenaceae species: alternative sources of (E)-Caryophyllene and germacrene-D. Quim. Nova 34, 1550–1555 (2011)

    Article  Google Scholar 

  42. Boubsia, N., Abert Vian, M., Ferhat, M.A., Peticolas, E., Meklati, B.Y., Chemat, F.: Comparison of two isolation methods for essential oil from rosemary leaves: hydrodistillation and microwave hydrodiffusion and gravity. Food Chem. 114, 355–362 (2009)

    Article  Google Scholar 

  43. Li, X.J., Wang, W., Luo, M., Li, C.Y., Zu, Y.G., Mu, P.S., Fu, Y.-J.: Solvent-free microwave extraction of essential oil from Dryopteris fragrans and evaluation of antioxidant activity. Food Chem. 133, 437–444 (2012)

    Article  Google Scholar 

  44. Zill-e-Huma, Vian, M.A., Fabiano-Tixier, A.S., Elmaataoui, M., Dangles, O., Chemat, F.: A remarkable influence of microwave extraction: enhancement of antioxidant activity of extracted onion varieties. Food Chem. 127, 1472–1480 (2011)

    Article  Google Scholar 

  45. Khan, M.K., Abert-Vian, M., Fabiano-Tixier, A.S., Dangles, O., Chemat, F.: Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chem. 115, 851–858 (2010)

    Article  Google Scholar 

  46. Morelli, L.L.L., Prado, M.A.: Extraction optimization for antioxidant phenolic compounds in red grape jam using ultrasound with a response surface methodology. Ultrason. Sonochem. 19, 1144–1149 (2012)

    Article  Google Scholar 

  47. Popović, B.M., Štajner, D., Slavko, K., Sandra, B.: Antioxidant capacity of cornelian cherry (Cornus mas L.)—comparison between permanganate reducing antioxidant capacity and other antioxidant methods. Food Chem. 134, 734–741 (2012)

    Article  Google Scholar 

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Acknowledgments

This study is a part of VALBOIS project which is financially supported by “Région Poitou-Charentes”. The authors would acknowledge this financial support as well as Achimbaud Company for providing the wood waste.

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Authors and Affiliations

  1. Pôles Sciences et Technologie, Université de La Rochelle, LaSIE, FRE-CNRS 3474, Avenue Michel Crépeau, 17042, La Rochelle, France

    A. Meullemiestre, Z. Maache-Rezzoug & S. A. Rezzoug

  2. Chemistry Department, Soran University, Soran, Kurdistan Region, Iraq

    I. Kamal

  3. Sécurité et Qualité des Produits d’Origine Végétale, Université d’Avignon et des Pays de Vaucluse, INRA, UMR408, 84000, Avignon, France

    F. Chemat

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  1. A. Meullemiestre
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Correspondence to S. A. Rezzoug.

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Meullemiestre, A., Kamal, I., Maache-Rezzoug, Z. et al. Antioxidant Activity and Total Phenolic Content of Oils Extracted from Pinus pinaster Sawdust Waste. Screening of Different Innovative Isolation Techniques. Waste Biomass Valor 5, 283–292 (2014). https://doi.org/10.1007/s12649-013-9237-8

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