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The potential curative and hepatoprotective effects of platelet rich plasma on liver fibrosis in Schistosoma mansoni experimentally infected mice

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Abstract

Trapped Schistosoma mansoni eggs trigger fibrotic liver disease that can continue to liver cirrhosis and failure. This work evaluates the outcome of platelet rich plasma (PRP) on S. mansoni-induced liver fibrosis by intraperitoneal (IP) and intrahepatic (IH) routes with/without Praziquantel (PZQ) treatment. Swiss albino mice (n = 162) were divided into non-infected (n = 66) and infected (n = 96) groups, then subdivided into non-treated and treated subgroups with PRP(IP), PRP(IH) 6th and 10th weeks post-infection, PZQ, PZQ + PRP(IP) and PZQ + PRP(IH) 6th and 10th weeks post-infection. Effects of treatments were evaluated by parasitological, histopathological and Immunohistochemical assessments. In the early assessment (12th week post-infection) of infected-treated groups, the mean granuloma number showed significant reduction in groups treated with PZQ + PRP (IH) 10th week, PRP (IP), PZQ + PRP (IP) and PZQ + PRP (IH) 6th week (33.33%, 33%, 27.77% and 27.22%, respectively). Furthermore, the mean granuloma diameter showed significant reduction in groups treated with PRP (IH) 10th week and PZQ + PRP (IP) (24.17% and 15.5%, respectively). Also, the fibrotic index showed significant reduction in groups treated with PZQ + PRP (IP), PRP (IP) and PZQ + PRP (IH) 6th week (48.18%, 46.81% and 41.36%, respectively). Transforming growth factor β1(TGF-β1) expression was in correlation with parasitological and histopathological results. Diminished TGF-β1 expression was mostly in infected groups treated with PZQ + PRP (IP), PZQ + PRP (IH) 6th week and PRP (IP) (88.63%, 88.63% and 77.27%, respectively). In the late assessment (14th week post-infection) of infected treated groups, TGF-β1expression was reduced in groups treated with PZQ, PRP (IH) 10th weeks, PRP (IP) (83.33%, 66.66%, 33.33% respectively). PRP showed promising anti-fibrotic effects on S. mansoni-induced liver fibrosis.

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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Medical Parasitology Department, Faculty of Medicine, Damietta University, Damietta, Egypt

    Angham M. A. Bayoumi

  2. Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Kasr Al-Ainy St, Cairo, 11562, Egypt

    Mousa A. M. Ismail & Hebat-Allah S. A. Yousof

  3. Parasitology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt

    Soheir S. Mahmoud

  4. Pathology Department, National Research Centre, Cairo, Egypt

    Ahmed S. A. Soliman

  5. Pathology Department, Faculty of Medicine, October 6 University, 6th of October City, Egypt

    Amr M. A. Mousa

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All manuscript authors participated to every activity of it, research idea and design, research methods, writing the article and reviewing it. All authors contributed to the study conception and design. Material preparation, data collection and analysis. All authors read and approved the final manuscript.

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Correspondence to Hebat-Allah S. A. Yousof.

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This study was granted by the research ethics committee (REC) for human and animal research, Faculty of Medicine, Cairo University (Code: MD-204-2022).

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Bayoumi, A.M.A., Ismail, M.A.M., Mahmoud, S.S. et al. The potential curative and hepatoprotective effects of platelet rich plasma on liver fibrosis in Schistosoma mansoni experimentally infected mice. J Parasit Dis 47, 349–362 (2023). https://doi.org/10.1007/s12639-023-01576-9

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