Skip to main content
SpringerLink
Log in

New Nano-Bioactive Glass/Magnesium Phosphate Composites by Sol-Gel Route for Bone Defect Treatment

  • Original Paper
  • Published:
Silicon Aims and scope Submit manuscript

Abstract

This work was mainly aimed to synthesize new ceramic composites based on nano-bioactive glass (nBG) and magnesium phosphate ceramic (MgP) with different ratios using the sol-gel approach in order to overcome the limitations of both materials. The glass was based on 85SiO2-10CaO-5P2O5 (mole %), and MgP was based on the formula; Mg3(PO4)2. The obtained composites were characterized by DTA, XRD, FTIR, SEM/EDX and Zeta potential techniques. The in vitro bioactivity test was carried out in SBF, and the cell viability test was conducted by culturing the composites with human normal fibroblast cell line (BJ1). The results of XRD analyses showed that there were often no strong diffraction peaks detected which indicated the amorphous nature of the ceramic composites. Moreover, soaking of the ceramic composites in SBF exhibited that addition of MgP to nBG was increased the degradation of the latter one, and nBG was improved the formation of apatite crystals on MgP ceramic. Moreover, the cell viability results showed that MgP was showed significant higher viability than nBG at high concentrations, and it improved the viability of nBG in the ceramic composites.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wang W, Yeung KW (2017) Bone grafts and biomaterials substitutes for bone defect repair: a review. Bioactive materials 2(4):224–247

    Article  Google Scholar 

  2. Dimitriou R, Mataliotakis GI, Angoules AG, Kanakaris NK, Giannoudis PV (2011) Complications following autologous bone graft harvesting from the iliac crest and using the RIA: a systematic review. Injury 42:S3–S15

    Article  Google Scholar 

  3. Giannoudis PV, Dinopoulos H, Tsiridis E (2005) Bone substitutes: an update. Injury 36(3):S20–S27

    Article  Google Scholar 

  4. Zimmermann G, Moghaddam A (2011) Allograft bone matrix versus synthetic bone graft substitutes. Injury 42:S16–S21

    Article  Google Scholar 

  5. LeGeros RZ (2008) Calcium phosphate-based osteoinductive materials. Chem Rev 108(11):4742–4753

    Article  Google Scholar 

  6. Jongman Lee MMF (2014) Eui Kyun Park, Jiwon Lim, Hui-suk Yun, A simultaneous process of 3D magnesiumphosphate scaffold fabrication and bioactive substance loading for hard tissue regeneration. Mater Sci Eng C 36:52–260

    Google Scholar 

  7. M.M. Farag, H.-s.Y., Effect of gelatin addition on fabrication of magnesium phosphate-based scaffolds prepared by additive manufacturing system. Mater Lett, 2014. 132: p. 111–111, 115

  8. Hench LL, Jones JR (2015) Bioactive glasses: frontiers and challenges. Frontiers in bioengineering and biotechnology 3:194

    Article  Google Scholar 

  9. Bellucci D, Sola A, Anesi A, Salvatori R, Chiarini L, Cannillo V (2015) Bioactive glass/hydroxyapatite composites: mechanical properties and biological evaluation. Mater Sci Eng C 51:196–205

    Article  CAS  Google Scholar 

  10. Moseke C, Saratsis V, Gbureck U (2011) Injectability and mechanical properties of magnesium phosphate cements. J Mater Sci Mater Med 22(12):2591–2598

    Article  CAS  Google Scholar 

  11. Yu Y, Wang J, Liu C, Zhang B, Chen H, Guo H, Zhong G, Qu W, Jiang S, Huang H (2010) Evaluation of inherent toxicology and biocompatibility of magnesium phosphate bone cement. Colloids Surf B: Biointerfaces 76(2):496–504

    Article  CAS  Google Scholar 

  12. Tamimi F, Nihouannen DL, Bassett DC, Ibasco S, Gbureck U, Knowles J, Wright A, Flynn A, Komarova SV, Barralet JE (2011) Biocompatibility of magnesium phosphate minerals and their stability under physiological conditions. Acta Biomater 7(6):2678–2685

    Article  CAS  Google Scholar 

  13. Zreiqat H, Howlett CR, Zannettino A, Evans P, Schulze-Tanzil G, Knabe C, Shakibaei M (2002) Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials 62(2):175–184

    Article  CAS  Google Scholar 

  14. Yamasaki Y, Yoshida Y, Okazaki M, Shimazu A, Uchida T, Kubo T, Akagawa Y, Hamada Y, Takahashi J, Matsuura N (2002) Synthesis of functionally graded MgCO3 apatite accelerating osteoblast adhesion. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials 62(1):99–105

    Article  CAS  Google Scholar 

  15. Cao W, Hench LL (1996) Bioactive materials. Ceram Int 22(6):493–507

    Article  CAS  Google Scholar 

  16. Hench LL, Splinter RJ, Allen WC, Greenlee TK (1971) Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res 5(6):117–141

    Article  Google Scholar 

  17. Ojansivu M, Vanhatupa S, Björkvik L, Häkkänen H, Kellomäki M, Autio R, Ihalainen JA, Hupa L, Miettinen S (2015) Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells. Acta Biomater 21:190–203

    Article  CAS  Google Scholar 

  18. Balasubramanian P, Hupa L, Jokic B, Detsch R, Grünewald A, Boccaccini AR (2017) Angiogenic potential of boron-containing bioactive glasses: in vitro study. J Mater Sci 52(15):8785–8792

    Article  CAS  Google Scholar 

  19. Vallet-Regí M, Ragel CV, Salinas AJ (2003) Glasses with medical applications. Eur J Inorg Chem 2003(6):1029–1042

    Article  Google Scholar 

  20. Bellucci D, Sola A, Salvatori R, Anesi A, Chiarini L, Cannillo V (2014) Sol–gel derived bioactive glasses with low tendency to crystallize: synthesis, post-sintering bioactivity and possible application for the production of porous scaffolds. Mater Sci Eng C 43:573–586

    Article  CAS  Google Scholar 

  21. Ostrowski N, Roy A, Kumta PN (2016) Magnesium phosphate cement systems for hard tissue applications: a review. ACS Biomaterials Science & Engineering 2(7):1067–1083

    Article  CAS  Google Scholar 

  22. Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27(15):2907–2915

    Article  CAS  Google Scholar 

  23. Bassyouni FA et al (2014) Synthesis and biological evaluation of some new triazolo [1, 5-a] quinoline derivatives as anticancer and antimicrobial agents. RSC Adv 4(46):24131–24141

    Article  CAS  Google Scholar 

  24. Suzuki T, Yamamoto T, Toriyama M, Nishizawa K, Yokogawa Y, Mucalo MR, Kawamoto Y, Nagata F, Kameyama T (1997) Surface instability of calcium phosphate ceramics in tissue culture medium and the effect on adhesion and growth of anchorage-dependent animal cells. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials and The Japanese Society for Biomaterials 34(4):507–517

    Article  CAS  Google Scholar 

  25. Ohgaki M, Kizuki T, Katsura M, Yamashita K (2001) Manipulation of selective cell adhesion and growth by surface charges of electrically polarized hydroxyapatite. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials 57(3):366–373

    Article  CAS  Google Scholar 

  26. Teng N et al (2001) A new approach to enhancement of bone formation by electrically polarized hydroxyapatite. J Dent Res 80(10):1925–1929

    Article  CAS  Google Scholar 

  27. Smith I, Baumann M, McCabe L (2004) Electrostatic interactions as a predictor for osteoblast attachment to biomaterials. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials 70(3):436–441

    Article  CAS  Google Scholar 

  28. Sarkar K, Rahaman M, Agarwal S, Bodhak S, Halder S, Nandi SK, Roy M (2020) Degradability and in vivo biocompatibility of doped magnesium phosphate bioceramic scaffolds. Mater Lett 259:126892

    Article  CAS  Google Scholar 

  29. Wang Z, Ma Y, Wei J, Chen X, Cao L, Weng W, Li Q, Guo H, Su J (2017) Effects of sintering temperature on surface morphology/microstructure, in vitro degradability, mineralization and osteoblast response to magnesium phosphate as biomedical material. Sci Rep 7(1):823

    Article  Google Scholar 

  30. Farag MM, Al-Rashidy ZM, Ahmed MM (2019) In vitro drug release behavior of Ce-doped nano-bioactive glass carriers under oxidative stress. J Mater Sci Mater Med 30(2):18

    Article  Google Scholar 

  31. Jia J, Zhou H, Wei J, Jiang X, Hua H, Chen F, Wei S, Shin JW, Liu C (2010) Development of magnesium calcium phosphate biocement for bone regeneration. J R Soc Interface 7(49):1171–1180

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was funded by Science and Technology Development Fund (STDF) under the project no. 24217. The authors would like to thank the National Research Centre (Biomaterials Group) and University of California Riverside, USA, for a possibility to use their facilities.

Author information

Authors and Affiliations

  1. Glass Research Department, National Research Centre, 33 El Bohouth Str., Dokki, Giza, 12622, Egypt

    M. M. Farag

  2. Department of Bioengineering, MSE227 Materials Science and Engineering Building, University of California, Riverside, Riverside, CA, 92521, USA

    H. H. Liu

  3. Manufacturing Engineering Department, College of Engineering and Computer Science, University of Texas Pan-American, Edinburg, TX, 78539-2999, USA

    A.H. Makhlouf

Authors
  1. M. M. Farag
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. H. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A.H. Makhlouf
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. M. Farag.

Ethics declarations

Conflict of Interest

The authors declare that there have no conflicts of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Farag, M.M., Liu, H.H. & Makhlouf, A. New Nano-Bioactive Glass/Magnesium Phosphate Composites by Sol-Gel Route for Bone Defect Treatment. Silicon 13, 857–865 (2021). https://doi.org/10.1007/s12633-020-00485-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12633-020-00485-3

Keywords

Search

Navigation