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Effect of Dietary Probiotic Lactobacillus helveticus on Growth Performance, Antioxidant Levels, and Absorption of Essential Trace Elements in Goldfish (Carassius auratus)

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Abstract

In this study, we have investigated the effect of probiotic Lactobacillus helveticus CD6 supplemented into simple laboratory fish feed (LFF) and complex, multi-ingredient market fish feed (MFF) on growth performance, antioxidant levels, and essential trace element absorption in goldfish (Carassius auratus). Twenty-four healthy goldfish (average weight 3–4 g) were acclimatized and divided into four experimental diets supplemented with 3 × 107 CFU/g of probiotic (LFF + Pro, MFF + Pro) and without probiotic (LFF, MFF) for 14 weeks. Fish fed with probiotic were healthy, active, and intense orange-gold as compared to control (without probiotic). At 14 weeks, fish fed with MFF + Pro/LFF + Pro showed 91/47% weight gain as compared to 34/− 12% weight observed with MFF/LFF. The average weight gain differences recorded between probiotic and control diets were not significant. No mortality to report when fish fed with probiotic. In contrast, fish fed without probiotic showed mortalities (LFF, two fish; MFF, one fish) during the trial. DPPH activity revealed high levels of antioxidants into the intestine of probiotic-fed fish. Trace element analysis showed that probiotic colonization enhanced diet-dependent absorption of Fe and Zn. The in vitro antimicrobial activity was exhibited by probiotic L. helveticus CD6 against infected fish isolate Aeromonas spp. JA showed an ability to protect fish from infections. Moreover, complex, multi-ingredient feed had a highest impact on viability of probiotic during storage. In conclusion, L. helveticus CD6 did not significantly enhance growth performance; however, it improved health and reduced mortalities in goldfish (C. auratus) regardless of the composition of the diet.

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Acknowledgements

Patil DK is gratefully acknowledged for his assistance during the study.

Funding

Ahire JJ received a financial support from UGC-RFSMS (Grant No.: F.4-1/2006 (BSR)/7-137/2007(BSR)), New Delhi, during Ph.D. (2010–2012) at North Maharashtra University, Jalgaon, India.

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Author notes

  1. J. J. Ahire

    Present address: Department of Microbiology, University of Stellenbosch, JC Smuts Building, Van der Bijl Street, Matieland, Stellenbosch, 7602, South Africa

Authors and Affiliations

  1. Department of Microbiology, School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, 425001, India

    J. J. Ahire, N. U. Mokashe & B. L. Chaudhari

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  2. N. U. Mokashe
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Correspondence to J. J. Ahire.

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All institutional and national guidelines for the care and use of laboratory animals were followed.

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Ahire, J.J., Mokashe, N.U. & Chaudhari, B.L. Effect of Dietary Probiotic Lactobacillus helveticus on Growth Performance, Antioxidant Levels, and Absorption of Essential Trace Elements in Goldfish (Carassius auratus). Probiotics & Antimicro. Prot. 11, 559–568 (2019). https://doi.org/10.1007/s12602-018-9428-5

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