Abstract
The study aims to evaluate the cell-free supernatant (CFS) from Lactobacillus plantarum strain MYS44 against the growth and aflatoxin production by Aspergillus parasiticus MTCC 411. Standard in vitro techniques revealed the potential antifungal activity of CFS of LpMYS44. In poison food technique, it was observed that 6% CFS of LpMYS44 retarded maximum growth. The inhibition of A. parasiticus on peanuts confirmed the ability of CFS of LpMYS44 for biopreservation. Further, CFS of LpMYS44 was purified by chromatography and analyzed by GC-MS. The major antifungal compounds were oleic acid, octanoic acid, butanamide, and decanoic acid derivatives. Twofold concentrated 80 μL of CFS was found to be minimum inhibitory concentration (MIC) of CFS of LpMYS44. CFS of LpMYS44 suppressed the germination and growth of the spores of A. parasiticus. Microscopic observation showed that CFS of LpMYS44 severely affected the hyphal wall of A. parasiticus by the leakage of cytoplasmic content leading to complete destruction. Acidic condition is favorable for CFS of LpMYS44 activity. In poultry feed sample, CFS of LpMYS44 reduced the aflatoxin B1 content by 34.2%, reflecting its potentiality to use as detoxification agent. The multiple antifungal components in CFS of LpMYS44 exhibited antifungal properties against aflatoxigenic A. parasiticus resulted in causing overall morphological changes. Furthermore, we also observed the biopreservative ability of CFS of LpMYS44 against A. parasiticus and AFB1 reduction in for poultry feed. This study makes a contribution to using CFS of LpMYS44 and their applications in food and feed as pretreatment against aflatoxigenic A. parasiticus to reduce or eliminate AFB1 and maybe other aflatoxins, produced by other Aspergillus spp.
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References
Yu J, Whitelaw C, NiermanWC BD, Cleveland TE (2004) Aspergillus flavus expressed sequence tags for identification of genes with putative roles in aflatoxin contamination of crops. FEMS Microbiol Lett 237:333–340
Rodrigues P, Venâncio A, Kozakiewicz Z, Lima N (2009) A polyphasic approach to the identification of aflatoxigenic and non-aflatoxigenic strains of Aspergillus Section Flavi isolated from Portuguese almonds. Int J Food Microbiol 129:187–193
Gerbaldo G, Barberis C, Pascual L, Dalcero A, Barberis L (2012) Antifungal activity of two Lactobacillus strains with potential probiotic properties. FEMS Microbiol Lett 332:27–33
Khanafari A, Soudi H, Miraboulfathi M (2007) Biocontrol of Aspergillus flavus and aflatoxin B1 production in corn. Iran J Environ Health Sci Eng 4:163–168
Guan D, Li P, Zhang Q, Zhang W, Zhang D, Jiang J (2011) An ultra-sensitive monoclonal antibody-based competitive enzyme immunoassay for aflatoxin M1 in milk and infant milk products. Food Chem 125:1359–1364
El-aziz ARMA, Mahmoud MA, Al-othman MR, Al-gahtani MF (2015) Use of selected essential oils to control aflatoxin contaminated stored cashew and detection of aflatoxin biosynthesis gene. Sci World J 1–13
Huwig A, Freimund S, Käppeli O, Dutler H (2001) Mycotoxin detoxication of animal feed by different adsorbents. Toxicol Lett 122(2):179–188
Yang EJ, Chang HC (2010) Purification of a new antifungal compound produced by Lactobacillus plantarum AF1 isolated from kimchi. Int J Food Microbiol 139:56–63
Wang H, Yan H, Shin J, Huang L, Zhang H, Qi W (2011) Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang koumiss in China. Ann Microbiol 61:879–885
Dalié DK, Deschamps AM, Atanasova-Penichon V, Richard-Forget F (2010) Potential of Pediococcus pentosaceus (L006) isolated from maize leaf to suppress fumonisin-producing fungal growth. J Food Prot 73(6):1129–1137
Ahmad Rather I, Seo BJ, Rejish Kumar VJ, Choi UH, Choi KH, Lim JH, Park YH (2013) Isolation and characterization of a proteinaceous antifungal compound from Lactobacillus plantarum YML007 and its application as a food preservative. Lett Appl Microbiol 57(1):69–76
Corsetti A, Gobbetti M, Rossi J, Damiani P (1998) Antimould activity of sourdough lactic acid bacteria: identification of a mixture of organic acids produced by Lactobacillus sanfrancisco CB1. Appl Microbiol Biotechnol 50(2):253–256
Strom K, Sjogren J, Broberg A, Schnurer J (2002) Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo (L-Phe-L-Pro) and cyclo (L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Appl Environ Microbiol 68(9):4322–4327
Sangmanee P, Hongpattarakere T (2014) Inhibitory of multiple antifungal components produced by Lactobacillus plantarum K35 on growth, aflatoxin production and ultrastructure alterations of Aspergillus flavus and Aspergillus parasiticus. Food Control 40:224–233
Kachouri F, Ksontini H, Hamdi M (2014) Removal of aflatoxin B1 and inhibition of Aspergillusflavus growth by the use of Lactobacillus plantarum on olives. J Food Prot 77:1760–1767
Deepthi BV, Rao KP, Chennapa G, Naik MK, Chandrashekara KT, Sreenivasa MY (2016) Antifungal attributes of Lactobacillus plantarum MYS6 against fumonisin producing Fusarium proliferatum associated with poultry feeds. PloS One 11(6):e0155122
Poornachandra Rao K, Chennappa G, Suraj U, Nagaraja H, Charith Raj AP, Sreenivasa MY (2015) Probiotic potential of Lactobacillus strains isolated from sorghum-based traditional fermented food. Probiotics Antimicrob Proteins 7(2):146–156
Gerez CL, Torres MJ, Font de Valdez G, Rollán G (2013) Control of spoilage fungi by lactic acid bacteria. Biol Control 64:231–237
Wang H, Yan Y, Wang J, Zhang H, Qi W (2012) Production and characterization of antifungal compounds produced by Lactobacillus plantarum IMAU10014. PLoS One 7:e29452
Ilavenil S, Park HS, Vijayakumar M, Valan Arasu M, Kim DH, Ravikumar S, Choi KC (2015) Probiotic potential of Lactobacillus strains with antifungal activity isolated from animal manure. Sci World J
Arasu MV, Kim DH, Kim PI, Jung MW, Ilavenil S, Jane M, Choi KC (2014) In vitro antifungal, probiotic and antioxidant properties of novel Lactobacillus plantarum K46 isolated from fermented sesame leaf. Ann Microbiol 64(3):1333–1346
Schillinger U, Villarreal JV (2010) Inhibition of Penicillium nordicumin MRS medium by lactic acid bacteria isolated from foods. Food Control 21(2):107–111
De Muynck C, Leroy AI, De Maeseneire S, Arnaut F, Soetaert W, Vandamme EJ (2004) Potential of selected lactic acid bacteria to produce food compatible antifungal metabolites. Microbiol Res 159(4):339–346
Mauch A, Dal Bello F, Coffey A, Arendt EK (2010) The use of Lactobacillus brevis PS1 to in vitro inhibit the outgrowth of Fusarium culmorum and other common Fusarium species found on barley. Int J Food Microbiol 141(1):116–121
Crowley S, Mahony J, van Sinderen D (2013) Current perspectives on antifungal lactic acid bacteria as natural bio-preservatives. Trends Food Sci Technol 33(2):93–109
Guo J, Mauch A, Galle S, Murphy P, Arendt EK, Coffey A (2011) Inhibition of growth of Trichophyton tonsurans by Lactobacillus reuteri. J Appl Microbiol 111(2):474–483
Adebayo CO, Aderiye BI (2011) Suspected mode of antimycotic action of brevicin SG1 against Candida albicans and Penicillium citrinum. Food Control 22(12):1814–1820
Mandal V, Sen SK, Mandal NC (2013) Production and partial characterization of an inducer-dependent novel antifungal compound(s) by Pediococcus acidilactici LAB 5. J Sci Food Agric 93(10):2445–2453
Walters D, Raynor L, Mitchell A, Walker R, Walker K (2004) Antifungal activities of four fatty acids against plant pathogenic fungi. Mycopathologia 157:87–90
Corcoran BM, Stanton C, Fitzgerald GF, Ross RP (2007) Growth of probiotic Lactobacilli in the presence of oleic acid enhances subsequent survival in gastric juice. Microbiology 153:291–299
Al-Naseri A, Bowman JP, Wilson R, Nilsson RE, Britz ML (2013) Impact of lactose starvation on the physiology of Lactobacillus casei GCRL163 in the presence or absence of tween 80. J Proteome Res 12:5313–5322
Jiang Y, Liu W, Zou H, Cheng T, Tian N, Xian M (2014) Microbial production of short-chain diols. Microb Cell Factories 13(1):165
Sjogren J, Magnusson J, Broberg A, Schnürer J, Kenne L (2003) Antifungal 3-hydroxy fatty acids from Lactobacillus plantarum MiLAB 14. Appl Environ Microbiol 69(12):7554–7557
Gourama H, Bullerman LB (1995) Antimycotic and antiaflatoxigenic effect of lactic acid bacteria: a review. J Food Prot 58(11):1275–1280
Karunaratne A, WezenbergE BLB (1990) Inhibition of mold growth and aflatoxin production by Lactobacillus sp. J Food Prot 53(3):230–236
Zinedine A, Faid M, Benlemlih M (2005) In vitro reduction of aflatoxin B1 by strains of lactic acid bacteria isolated from Moroccan sourdough bread. Int J Agric Biol 7:67–70
Kumar SN, Sreekala SR, Chandrasekaran D, Nambisan B, Anto RJ (2014) Biocontrol of Aspergillus species on peanut kernels by antifungal diketopiperazine producing Bacillus cereus associated with entomopathogenic nematode. PLoS One 9(8):e106041
Acknowledgments
We thank the Central Instrumentation Facility, Institute of Excellence (IOE), University of Mysore for providing timely assistance for fluorescent microscopy. We also thank the SERB (Science and Engineering Research Board), India (No. EEQ/2016/000273; dated 23/02/2017) for their valuable support.
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Poornachandra Rao, K., Deepthi, B.V., Rakesh, S. et al. Antiaflatoxigenic Potential of Cell-Free Supernatant from Lactobacillus plantarum MYS44 Against Aspergillus parasiticus . Probiotics & Antimicro. Prot. 11, 55–64 (2019). https://doi.org/10.1007/s12602-017-9338-y
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DOI: https://doi.org/10.1007/s12602-017-9338-y