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Probiotic Potential of Autochthonous Bacteria Isolated from the Gastrointestinal Tract of Four Freshwater Teleosts

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Abstract

In this study, a total of 121 bacterial strains were isolated from the gastrointestinal tract of four teleostean species, namely striped snakehead (Channa striatus), striped dwarf catfish (Mystus vittatus), orangefin labeo (Labeo calbasu) and mrigal carp (Cirrhinus mrigala), among which 8 isolates showed promising antibacterial activity against four potential fish pathogens, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas sobria and Pseudomonas fluorescens and were non-hemolytic. The isolates were further screened in response to fish bile tolerance and extracellular digestive enzyme activity. Two bacterial strains MVF1 and MVH7 showed highest tolerance and extracellular enzymes activities, and selected for further studies. Antagonistic activity of these two isolates was further confirmed by in vitro growth inhibition assay against four selected fish pathogens in liquid medium. Finally, these two bacterial strains MVF1 and MVH7 were selected as potential probiotic candidates and thus identification by partial 16S rRNA gene sequence analysis. The bacterial isolates MVF1 and MVH7 were identified as two strains of Bacillus sp.

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Acknowledgments

This work is a part of the PhD degree of the first author, and the authors are thankful to the Head, Department of Zoology, Visva-Bharati University, West Bengal, India, for providing the necessary facilities to carry out the present investigation. The first author is grateful to the Department of science and Technology (DST), New Delhi, India, for awarding the DST-INSPIRE research fellowship (Registration Number: IF120680).

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Authors and Affiliations

  1. Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, West Bengal, 731235, India

    Ankita Nandi, Suhas Kumar Dan, Goutam Banerjee, Pinki Ghosh & Arun Kumar Ray

  2. Department of Zoology, University of Burdwan, Golap Bag, Burdwan, West Bengal, 713 104, India

    Koushik Ghosh

  3. Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, 9037, Breivika, Norway

    Einar Ringø

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  1. Ankita Nandi
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  2. Suhas Kumar Dan
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  5. Koushik Ghosh
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Correspondence to Arun Kumar Ray.

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Nandi, A., Dan, S.K., Banerjee, G. et al. Probiotic Potential of Autochthonous Bacteria Isolated from the Gastrointestinal Tract of Four Freshwater Teleosts. Probiotics & Antimicro. Prot. 9, 12–21 (2017). https://doi.org/10.1007/s12602-016-9228-8

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