Abstract
Escherichia coli strains from swine origin, either susceptible or resistant to colistin, were grown under planktonic and biofilm cultures. After which, they were treated with antibacterial agents including nisin and enterocin DD14 bacteriocins, colistin and their combinations. Importantly, the combination of colistin, enterocin DD14 and nisin eradicated the planktonic and biofilm cultures of E. coli CIP54127 and the E. coli strains with colistin-resistance phenotype such as E. coli 184 (mcr-1 +) and E. coli 289 (mcr-1 −), suggesting therefore that bacteriocins from lactic acid bacteria could be used as agents with antibiotic augmentation capability.
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Acknowledgments
Ahmed Khassaf Al Atya received a PhD scholarship from the French and Iraqi governments. The authors are grateful to Campus France for providing funds and Lille 1 University for supporting visiting professorship position for Prof. Hikmate Abriouel. The authors wish to thank the RESAPATH laboratories, Dr. Sophie Granier (Anses) for the mcr-1 positive control and Dr. Benoit Cudennec for statistical analysis.
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Ahmed K. Al Atya, Hikmate Abriouel, Isabelle Kempf, Eric Jouy, Eric Auclair, Anne Vachée and Djamel Drider declare no conflict of interest.
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Al Atya, A.K., Abriouel, H., Kempf, I. et al. Effects of Colistin and Bacteriocins Combinations on the In Vitro Growth of Escherichia coli Strains from Swine Origin. Probiotics & Antimicro. Prot. 8, 183–190 (2016). https://doi.org/10.1007/s12602-016-9227-9
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DOI: https://doi.org/10.1007/s12602-016-9227-9