Probiotics and Antimicrobial Proteins

, Volume 6, Issue 2, pp 73–81 | Cite as

Probiotic Potential of Lactobacillus plantarum LD1 Isolated from Batter of Dosa, a South Indian Fermented Food

Article

Abstract

Lactobacillus plantarum LD1 was isolated from dosa batter and identified by biochemical, physiological and genetic methods. Species level identification was done by 16S rDNA amplification and sequencing. The probiotic potential of strain LD1 was assessed by different standard parameters. Cell surface hydrophobicity was recorded to be 62 % with SAT value <0.007 M. Seventy-eight percent of viable count was found after treatment with simulated gastric juice containing pepsin (pH 2.0). Bile salt tolerance and bile salt hydrolase activity were also demonstrated by strain LD1. The culture supernatant was able to inhibit food-borne as well as clinical pathogenic microorganisms such as Staphylococcus aureus, Salmonella typhimurium, Shigella flexneri, Pseudomonas aeruginosa, urogenic Escherichia coli and Vibrio sp. Strain LD1 was found to be sensitive to most of the antibiotics used in the study. Since strain has been isolated from food source that is most typical of Southern India, it would be safe for further consumption in probiotic products.

Keywords

Lactic acid bacteria Fermented foods Probiotics Bacteriocins Food-borne pathogens 

References

  1. 1.
    Battcock M, Azam-Ali S (1998) Fermented fruits and vegetables: a global perspective. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  2. 2.
    Begley M, Hill C, Gahan CG (2006) Bile salt hydrolase activity in probiotics. Appl Environ Microbiol 72:1729–1738CrossRefGoogle Scholar
  3. 3.
    Bhardwaj A, Kaur G, Gupta H, Vij S, Malik RK (2011) Interspecies diversity, safety and probiotic potential of bacteriocinogenic Enterococcus faecium isolated from dairy food and human faeces. World J Microbiol Biotechnol 27:591–602CrossRefGoogle Scholar
  4. 4.
    Charteris WP, Kelly PM, Morelli L, Collins JK (1998) Antibiotic susceptibility of potentially probiotic Lactobacillus species. J Food Prot 61:1636–1643Google Scholar
  5. 5.
    Chavan JK, Kadam SS (1989) Nutritional improvement of cereals by sprouting. Critical Rev in Food Sci Nutr 28:401–437CrossRefGoogle Scholar
  6. 6.
    Chou L, Weimer B (1999) Isolation and characterization of acid and bile-tolerant isolates from strains of Lactobacillus acidophilus. J Dairy Sci 82:23–31CrossRefGoogle Scholar
  7. 7.
    Divya BJ, Varsha KK, Nampoothiri MK (2012) Newly isolated lactic acid bacteria with probiotic features for potential application in food industry. Appl Biochem Biotechnol 167:1314–1324CrossRefGoogle Scholar
  8. 8.
    Franz CM, Specht I, Haberer P, Holzapfel WH (2001) Bile salt hydrolase activity of Enterococci isolated from food: screening and quantitative determination. J Food Prot 64:725–729Google Scholar
  9. 9.
    Ganguli K, Walker WA (2011) Probiotics in the prevention of necrotizing enterocolitis. J Clin Gastroenterol 45:S133–S138CrossRefGoogle Scholar
  10. 10.
    Ganguly NK, Bhattacharya SK, Sesikeran B, Nair GB, Ramakrishna BS, Sachdev HPS, Batish VK, Kanagasabapathy AS, Muthuswamy V, Kathuria SC, Katoch VM, Satyanarayana K, Toteja GS, Rahi M, Rao S, Bhan MK, Kapur R, Hemalatha R (2011) ICMR-DBT guidelines for evaluation of probiotics in food. Indian J Med Res 134:22–25Google Scholar
  11. 11.
    Ghosh N, Kumar M, Tiwari SK, Srivastava S (2008) Probiotic potential of two isolates of lactic acid bacteria, Lactobacillus plantarum LR/14 and Enterococcus faecium LR/6. Int J Probio Prebio 3:1–6Google Scholar
  12. 12.
    Guarner F, Schaafsma G (1998) Probiotics. Int J Food Microbiol 39:237–238CrossRefGoogle Scholar
  13. 13.
    Jin LZ, Ho YW, Abdullah N, Ali MA, Jalaludin S (1996) Antagonistic effects of intestinal Lactobacillus isolates on pathogens of chicken. Lett Appl Microbiol 23:67–71CrossRefGoogle Scholar
  14. 14.
    Kumar M, Tiwari SK, Srivastava S (2010) Purification and characterization of Enterocin LR/6 a bacteriocin from Enterococcus faecium LR/6. Appl Biochem Biotechnol 160:40–49CrossRefGoogle Scholar
  15. 15.
    Lavilla- Larma L, Pérez-Pulido R, Martínez-Bueno M, Valdivia E (2013) Characterization of functional, safety, and gut survival related characteristics of Lactobacillus strains isolated from farmhouse goat’s milk cheeses. Int J Food Microbiol 163:136–145CrossRefGoogle Scholar
  16. 16.
    Liong MT, Shah NP (2005) Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. Int Dairy J 15:391–398CrossRefGoogle Scholar
  17. 17.
    Mattila-Sandholm T, Matto J, Saarela M (1999) Lactic acid bacteria with health claims-interactions and interference with gastrointestinal flora. Int Dairy J 91:25–35CrossRefGoogle Scholar
  18. 18.
    Mélançon D, Grenier D (2003) Production and properties of bacteriocin-like inhibitory substances from the swine pathogen Streptococcus suis Serotype 2. Appl Environ Microbiol 69:4482–4488CrossRefGoogle Scholar
  19. 19.
    Naidu AS, Bidlack WR, Clemens RA (1999) Probiotic spectra of lactic acid bacteria (LAB). CRC Crit Rev Food Sci Nutr 39:13–126CrossRefGoogle Scholar
  20. 20.
    Palanisamy BD, Rajendran V, Sathyaseelan S, Bhat R, Venkatesan BP (2012) Enhancement of nutritional value of finger millet-based food (Indian dosa) by co-fermentation with horse gram flour. Int J Food Sci Nutr 63:5–15CrossRefGoogle Scholar
  21. 21.
    Patil MM, Ajay P, Anand T, Ramana VK (2010) Isolation and characterization of lactic acid bacteria from curd and cucumber. Ind J Biotechnol 9:166–172Google Scholar
  22. 22.
    Schiffrin EJ, Brassart D, Servin AL, Rochat F, Donnet-Hughes A (1997) Immune modulation of blood leucocytes in humans by lactic acid bacteria: criteria for strain selection. Am J Clinical Nutr 66:515S–520SGoogle Scholar
  23. 23.
    Soni SK, Sandhu DK, Vilkhu KS, Kamra N (1986) Microbiological studies on dosa fermentation. Food Microbiol 3:45–53CrossRefGoogle Scholar
  24. 24.
    Spadaccini A, De-Fanis C, Sciampa G, Masciulli V, Pantaleone U, Di Virgili M, Magnarini C, Pizzicannella G (1996) Omeprazole versus ranitidine: short-term triple-therapy in patients with Helicobacter pylori-positive duodenal ulcers. Aliment Pharmacol Ther 10:829–831CrossRefGoogle Scholar
  25. 25.
    Tiwari SK (2008) Production, Purification and Characterization of plantaricin LR14: A Novel Two-Peptide Bacteriocin from Lactobacillus plantarum LR/14. PhD Thesis, Department of Genetics, University of Delhi South Campus, New Delhi, IndiaGoogle Scholar
  26. 26.
    Tiwari SK, Srivastava S (2008) Characterization of a bacteriocin from Lactobacillus plantarum strain LR/14. Food Biotechnol 22:241–267Google Scholar
  27. 27.
    Velazquez JB (2012) Recent Patents on Bacteriocins: Food and Biomedical Applications. Recent Pat DNA Gene Seq. Aug 16Google Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of GeneticsMaharshi Dayanand UniversityRohtakIndia

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