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Insecticidal potential of an endophytic fungus, Cladosporium uredinicola, against Spodoptera litura

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Abstract

The tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae) (Fabricius), is a polyphagous pest which has developed physiological resistance against a number of known insecticides. In order to reduce the load of synthetic insecticides, ecofriendly alternative strategies are needed. The present studies were conducted to evaluate the insecticidal potential of the endophytic fungus Cladosporium uredinicola isolated from Tinospora cordifolia (Thunb.) on S. litura. Development of S. litura was significantly prolonged when larvae were fed on diet amended with ethyl acetate extract of C. uredinicola at concentrations of 1.25–2.00 μl g−1. The negative effects of fungal toxin on development of S. litura further resulted in a significant reduction in adult emergence, longevity and reproductive potential at higher concentrations. A significantly higher number of adults showed morphological deformities when larvae were fed on diet amended with 2.00 μl g−1 concentration. The inhibitory effects on growth and development of S. litura indicate toxicity of ethyl acetate extract of C. uredinicola, which is further evidenced by reduced food utilization by larvae. The antifeedant and toxic effects of ethyl acetate extract of C. uredinicola observed in the present studies can be attributed to a bioactive compound produced by the fungus, so this study provides evidence that C. uredinicola isolated from T. cordifolia possesses anti-insect properties and may play an important role in protecting plants against insect pests.

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References

  • Abdel-Baky, N. F., Arafat, N. S., & Abdel-Salam, A. H. (1998). Three Cladosporium spp. as promising biological control candidates for controlling whiteflies (Bemisia spp.) in Egypt. Pakistan. Journal of Biological Science, 1, 188–195.

    Google Scholar 

  • Afkhami, M. E., & Rudgers, J. A. (2009). Endophyte-mediated resistance to herbivores depends on herbivore identity in the wild grass Festuca subverticillata. Environmental Entomology, 38, 1086–1095.

    Article  PubMed  Google Scholar 

  • Azevedo, J. L., Maccheroni, W., Jr., Pereira, J. O., & Araujo, W. L. (2000). Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electronic Journal of Biotechnology, 3, 40–65.

    Article  Google Scholar 

  • Bacon, C. W., Porter, J. K., & Robbins, J. D. (1986). Ergot toxicity from endophyte infected weed grasses: a review. Agronomy Journal, 78, 106–116.

    Article  CAS  Google Scholar 

  • Bahar, M. H., Backhouse, D., Gregg, P. C., & Mensah, R. (2011). Efficacy of a Cladosporium sp. fungus against Helicoverpa armigera (Lepidoptera: Noctuidae), other insect pests and beneficial insects of cotton. Biocontrol Science and Technology, 21, 1387–1397.

    Article  Google Scholar 

  • Ball, O. J. P., Coudron, T. A., Tapper, B. A., Davies, E., Trently, D., Bush, L. P., et al. (2006). The importance of host plant species, Neotyphodium endophyte isolate, and alkaloids on feeding by Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae. Journal of Economic Entomology, 99, 1462–1473.

    Article  PubMed  CAS  Google Scholar 

  • Baoyu, H., Hangu, Z., & Liu, C. (1997). Epizootic infection and spatial pattern within epizootic peak period of Cladosporium sp. to the population of Aleurocanthus spiniferus. Entomology Journal of East China, 6, 66–70.

    Google Scholar 

  • Carroll, G. C. (1991). Beyond pest deterrence. Alternative strategies and hidden costs of endophytic mutualisms in vascular plants. In J. H. Andrews & S. S. Monano (Eds.), Microbial ecology of leaves (pp. 358–378). New York, NY: Springer-Verlag.

    Chapter  Google Scholar 

  • Clay, K., Hardy, T. N., & Hammond, A. M., Jr. (1985). Fungal endophytes of Cyperus and their effects on an insect herbivore. American Journal of Botany, 72, 1284–1289.

    Article  Google Scholar 

  • Crawford, K. M., Land, J. M., & Rudgers, J. A. (2010). Fungal endophytes of native grasses decrease insect herbivore preference and performance. Oecologia, 164, 431–444.

    Article  PubMed  Google Scholar 

  • Daisy, B. H., Strobel, G. A., Castillo, U., Ezra, D., Sears, J., Weaver, D. A., et al. (2002). Naphthalene, an insect repellent, is produced by Muscodor vitigenus, a novel endophytic fungus. Microbiology, 148, 3737–3741.

    PubMed  CAS  Google Scholar 

  • Farias, A. R. N., & Filho, H. P. S. (1987). Ocorrencia de Cladosporium spp. infectando a masca branca Aleurothrixus aepim em mandioca no Estado da Bahia. Revista Brasileira Mandioca, 6, 79–80.

    Google Scholar 

  • Findlay, J. A., Buthelezi, S., Li, G., Seveck, M., & Miller, J. D. (1997). Insect toxins from an endophytic fungus from wintergreen. Journal of Natural Products, 60, 1214–1215.

    Article  CAS  Google Scholar 

  • Gilman, J. C. (2001). A manual of soil fungi. Delhi, India: Biotech Books.

    Google Scholar 

  • Hammon, K. E., & Faeth, S. H. (1992). Ecology of plant herbivore communities: a fungal component. Natural Toxins, 1, 197–208.

    Article  PubMed  CAS  Google Scholar 

  • Hernawati, H., Wiyono, S., & Santoso, S. (2011). Leaf endophytic fungi of chilli (Capsicum annuum) and their role in the protection against Aphis gossypii (Homoptera: Aphididae). Biodiversitas, 12, 187–191.

    Google Scholar 

  • Jeyarani, S., Banu, J. G., & Ramaraju, K. (2011). First record of natural occurrence of Cladosporium cladosporioides (Fresenius) de Vries and Beauveria bassiana (Bals..-Criv.) Vuill. on two spotted spider mite Tetranychus urticae Koch from India. Journal of Entomology, 8, 274–279.

    Article  Google Scholar 

  • Koul, O., Shankar, J. S., Mehta, N., Taneja, S. C., Tripathi, A. K., & Dhar, K. L. (1997). Bioefficacy of crude extracts of Aglaia species (Meliaceae) and some active fractions against lepidopteran larvae. Journal of Applied Entomology, 121, 245–248.

    Article  Google Scholar 

  • Koul, O., Singh, G., Singh, R., & Singh, J. (2005). Bioefficacy and Mode-of-Action of Aglaroxin B and Aglaroxin C from Aglaia elaeagnoidea (syn A. roxburghiana) against Helicoverpa armigera and Spodoptera litura. Biopesticide International, 1(12), 54–64.

    Google Scholar 

  • Lagowka, B. (1995). The biological control perspective of scale insects (Homoptera: Coccinea) on ornamental plants in glasshouses. Wiadomosci Entomologiczne, 14, 5–10.

    Google Scholar 

  • Morimoto, M., & Komai, K. (2006). Insect antifeedant activity of natural products and the structure-activity relationship of their derivatives. pp. 182–193. In: M. Morimoto & K. Komai [Eds.] Natural products for pest management. ACS Symposium Series, American Chemical Society.

  • Müller, C. B., & Krauss, J. (2005). Symbiosis between grasses and asexual fungal endophytes. Current Opinion in Plant Biology, 8, 450–456.

    Article  PubMed  Google Scholar 

  • Pan, W. Y., Chen, S. L., Lian, J. H., Qin, H. Z., & Lan, G. (1989). A preliminary report on control of Hemiberlesia pitysophila using Cladosporium cladosporioides. Forest Pests and Disease, 3, 22–23.

    Google Scholar 

  • Phan, V. K., Chau, V. M., Nguyen, T. D., La, V. K., Dan, T. H., Nguyen, H. N., et al. (2010). Aporphine alkaloids, clerodane diterpenes and other constituents from Tinospora cordifolia. Fitoterapia, 81, 485–489.

    Article  PubMed  Google Scholar 

  • Powell, R. G., & Petroski, R. J. (1992). Alkaloid toxins in endophyte-infected grasses. Natural Toxins, 1, 163–170.

    Article  PubMed  CAS  Google Scholar 

  • Qin, H., Ye, Z., Huang, S., Ding, J., & Luo, R. (2004). The correlations of the different host plants with preference level, life duration and survival rate of Spodoptera litura (Fabricius). Chinese Journal of Eco-Agriculture, 12, 40–42.

    Google Scholar 

  • Raps, A., & Vidal, S. (1998). Indirect effects of an unspecialized endophytic fungus on specialized plant herbivorous insect interactions. Oecologia, 114, 541–547.

    Article  Google Scholar 

  • Rojas, T., Pons, N., & Arnal, E. (1998). Cladosporium herbarum on whiteflies (Homoptera: Aleyrodidae) in Venezuela. Boletin de Entomologia Venezolana, 13, 57–65.

    Google Scholar 

  • Rowan, D. D., Hunt, M. B., & Gaynor, D. L. (1986). Peramine, a novel insect feeding deterrent from ryegrass infected with the endophyte Acremonium loliae. Journal of Chemical Society D: Chemical Communications, 142, 935–936.

    Article  Google Scholar 

  • Samways, M. J., & Grech, N. M. (1986). Assessment of the fungus Cladosporium oxysporum (Berk and Curt) as a potential biocontrol agent against certain homoptera. Agriculture, Ecosystems & Environment, 15, 231–239.

    Article  Google Scholar 

  • Sharma, M., Chadha, B. S., Kaur, M., Ghatora, S. K., & Saini, H. S. (2008). Molecular characterization of multiple xylanase producing thermophilic/thermotolerant fungi isolated from composting materials. Letters in Applied Microbiology, 46, 526–535.

    Article  PubMed  CAS  Google Scholar 

  • Singh, S. S., Pandey, S. C., Srivastava, S., Gupta, V. S., Patro, B., & Ghosh, A. C. (2003). Chemistry and medicinal properties of Tinospora cordifolia (Guduchi). Indian Journal of Pharmacology, 35, 83–91.

    CAS  Google Scholar 

  • Slansky, F., Jr. (1993). Nutritional ecology: The fundamental quest for nutrients. pp. 29–91. In N. E. Stamp & T. M. Casey (Eds.), Caterpillars: Ecological and evolutionary constraints on foraging. New York, NY: Chapman and Hall.

    Google Scholar 

  • Stone, J. K., Bacon, C. W., & White, J. F., Jr. (2000). An overview of endophytic microbes: Endophytism defined. pp. 3–29. In C. W. Bacon & J. F. White Jr. (Eds.), Microbial endophytes. New York, NY: Marcel Dekker.

    Google Scholar 

  • Sumarah, M. W., & Miller, J. D. (2009). Anti-insect secondary metabolites from fungal endophytes of conifer trees. Natural Product Communications, 4, 1497–1504.

    PubMed  CAS  Google Scholar 

  • Sumarah, M. W., Puniani, E., Sorensen, D., Blackwell, B. A., & Miller, J. D. (2010). Secondary metabolites from anti-insect extracts of endophytic fungi isolated from Picea rubens. Phytochemistry, 71, 760–765.

    Article  PubMed  CAS  Google Scholar 

  • Thakur, A., Kaur, S., Kaur, A., & Singh, V. (2012). Detrimental effects of endophytic fungus Nigrospora sp. on survival and development of Spodoptera litura. Biocontrol Science and Technology, 22, 151–161.

    Article  Google Scholar 

  • Thakur, A., Kaur, S., Kaur, A., & Singh, V. (2013). Enhanced resistance to Spodoptera litura in endophyte infected cauliflower plants. Environmental Entomology, 42(2). doi:10.1603/EN12001.

  • Thumar, R. K., & Kapadia, M. N. (1994). Ovicidal control of Aleurolobus barodensis (Maskell) and its suppression by the entomophagous fungus. Indian Sugar, 44, 501–502.

    Google Scholar 

  • Vallejo, L. F., Soto, S. U., & Bernal, T. D. V. (1996). Identification of pathogenic fungi for Lutzomyia sp. (Diptera: Psychodidae), vectors of Leishmaniasis. Revista Colombiana de Entomologia, 22, 13–17.

    Google Scholar 

  • Venkateswarlu, U. T., Madhumathi, P. A. R. R., & Rao, V. S. (2006). Status of insecticidal resistance in Spodoptera litura on cotton in Guntur and Prakasan District of Andhara Pradesh. Indian Journal of Plant Protection, 34, 26–32.

    CAS  Google Scholar 

  • Waldbauer, G. P. (1968). The consumption and utilization of food by insects. Advances in Insect Physiology, 5, 229–288.

    Article  Google Scholar 

  • Wheeler, D. A., & Isman, M. B. (2001). Antifeedant and toxic activity of Trichilia americana extract against the larvae of Spodoptera litura. Entomologia Experimentalis et Applicata, 98, 9–16.

    Article  Google Scholar 

  • Xie, Y. S., Isman, M. B., Gunning, P., MacKinnon, S., Arnason, J. T., Taylor, D. R., et al. (1994). Biological activity of extracts of Trichilia species and the limonoid hirtin against lepidopteran larvae. Biochemical Systematics and Ecology, 22, 129–136.

    Article  CAS  Google Scholar 

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Acknowledgments

Financial assistance from University Grants Commission (UGC), Government of India, New Delhi, is duly acknowledged.

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Authors and Affiliations

  1. Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India

    Abhinay Thakur & Sanehdeep Kaur

  2. Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India

    Varinder Singh & Amarjeet Kaur

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  1. Abhinay Thakur
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Correspondence to Sanehdeep Kaur.

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Thakur, A., Singh, V., Kaur, A. et al. Insecticidal potential of an endophytic fungus, Cladosporium uredinicola, against Spodoptera litura . Phytoparasitica 41, 373–382 (2013). https://doi.org/10.1007/s12600-013-0298-9

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