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Phylogenetic analysis of partial sequences from Fig mosaic virus isolates in Turkey

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Abstract

Fig mosaic virus (FMV), which was described recently, is the only characterized causal agent of fig mosaic disease (FMD). It has six RNA segments and belongs to the Bunyaviridae family. In order to determine the genetic diversity of Turkish FMV isolates, the most common fig cultivars showing FMD symptoms were collected from different fig-growing provinces of Turkey. Nucleoprotein (Np) and Glycoprotein (Gp) gene-specific primers of FMV were used for RT-PCR analysis. According to RT-PCR results, 71 of 90 samples from 20 different cultivars and unknown fig seedlings were found to be infected by FMV. Among them, 41 isolates were sequenced and subjected to phylogenetic analyses based on the partial Gp and Np sequences at the amino acid level by the neighbor-joining method. The isolates showed more than 80% identity with reference FMV isolates (Acc. nos. FM991954.1 and FM864225.2). Based on phylogenetic analysis, the sequences clustered into two main groups for Np and Gp regions. Significant relationships between FMV isolates based on geographic origin and cultivars were not observed.

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Acknowledgments

The authors would like to thank Prof. Dr. Bryce W. Falk and Dr. Jeewan Jyot Walia from the University of California-Davis for their kind help during the experiments. The authors also thank Prof. Dr. Anne Frary from İzmir Institute of Technology for the English editing of the manuscript. Eminur (Barutçu) Elçi is grateful to The Scientific and Technological Research Council of Turkey (TÜBİTAK) for International research fellowships (2214). This study was supported by TÜBİTAK-TOVAG 111O827 and Mustafa Kemal University-BAP-1202M0113 projects. Accession numbers: JQ173767–JQ173848

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  1. Agriculture Faculty, Plant Protection Department, Mustafa Kemal University, 31034, Antakya-Hatay, Turkey

    Eminur Elçi, Çiğdem Ulubaş Serçe & Kadriye Çağlayan

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  1. Eminur Elçi
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  2. Çiğdem Ulubaş Serçe
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  3. Kadriye Çağlayan
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Correspondence to Eminur Elçi.

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Elçi, E., Serçe, Ç.U. & Çağlayan, K. Phylogenetic analysis of partial sequences from Fig mosaic virus isolates in Turkey. Phytoparasitica 41, 263–270 (2013). https://doi.org/10.1007/s12600-013-0286-0

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  • DOI: https://doi.org/10.1007/s12600-013-0286-0

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