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Shift of sensitivity of Botrytis cinerea to azoxystrobin in greenhouse vegetables before and after exposure to the fungicide

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Abstract

From 2004 to 2006, 213 isolates of Botrytis cinerea never exposed to QO center inhibitors (QOIs) were collected to determine the baseline sensitivity to azoxystrobin. In the absence of salicylhydroxamic acid (SHAM), the mean EC50 values were 10.49 ± 13.12 and 0.36 ± 0.48 mg l−1 for inhibiting mycelial growth and conidium germination, respectively. In the presence of SHAM, the mean EC50 values were 2.24 ± 1.29 and 0.22 ± 0.11 mg l−1. In 2010, five azoxystrobin-resistant isolates were detected with the resistance frequency of 2.25% in greenhouse tomatoes after 4 years of continuous exposure. These resistant isolates showed cross-resistance to other QOIs but not to boscalid. In addition, these resistant isolates had comparable growth, sporulation and pathogenicity ability as sensitive isolates and maintained resistance in plants and the presence of SHAM. The G143A point mutation predicted to cause a change from glycine to alanine at codon 143 of cyt b gene was found in all resistant isolates.

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Acknowledgments

This research was part of Project 31071711 supported by NSFC, grant no. Y3090378, of the Zhejiang Natural Science Foundation, and Project 2010C32083 of the Zhejiang Public-Interest Technology Application Study.

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Authors and Affiliations

  1. College of Agriculture and Food Science, Zhejiang Agriculture & Forest University, Lin’an, China

    Chuan-qing Zhang, Ya-hui Liu & Guo-nian Zhu

  2. Extension Centre of Agriculture Technology of Lin’an City, Lin’an, Zhejiang Province, China

    Lan Ding

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  1. Chuan-qing Zhang
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  2. Ya-hui Liu
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  3. Lan Ding
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  4. Guo-nian Zhu
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Correspondence to Chuan-qing Zhang.

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Zhang, Cq., Liu, Yh., Ding, L. et al. Shift of sensitivity of Botrytis cinerea to azoxystrobin in greenhouse vegetables before and after exposure to the fungicide. Phytoparasitica 39, 293–302 (2011). https://doi.org/10.1007/s12600-011-0159-3

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