Skip to main content
SpringerLink
Log in

Evolution of resistance to different classes of fungicides in Botrytis cinerea from greenhouse vegetables in eastern China

  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Between 2003 and 2005, 337 isolates of Botrytis cinerea collected from greenhouse vegetables were characterized for resistance to fungicides. A low level of chlorothalonil resistance was detected and in these resistant isolates there was cross-resistance to captan and thiram. To the best of our knowledge, this is the first report of chlorothalonil resistance in B. cinerea from vegetables in China. The sub-population of B. cinerea highly resistant to benzimidazoles developed quickly during the years 2003 to 2005. Rapid spread of double resistance to benzimidazoles and diethofencarb was also observed. Resistance to dicarboximides was of low-level character and no highly resistant isolates were detected. In contrast, emergence of resistance to pyrimethanil, the only anilinopyrimidine fungicide used in China at present, was detected in 2003 just 3 years after pyrimethanil introduction. Pyrimethanil-resistant isolates demonstrated fitness comparable with that of wild sensitive isolates. These results suggest that pyrimethanil has a high risk of leading to resistance development in B. cinerea in greenhouse vegetables.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Barak, E., & Edgington, L. V. (1984). Cross-resistance of Botrytis cinerea to captan, thiram, chlorothalonil and related fungicides. Canadian Journal of Plant Pathology, 6, 318–320.

    CAS  Google Scholar 

  • Baroffio, C. A., Siegfried, W., & Hilber, U. W. (2003). Long-term monitoring for resistance of Botryotinia fuckeliana to anilinopyrimidine, phenylpyrrole, and hydroxyanilide fungicide in Switzerland. Plant Disease, 87, 662–666.

    Article  CAS  Google Scholar 

  • Beever, R. E., Laracy, E. P., & Park, H. A. (1989). Strain of Botrytis cinerea resistant to dicarboximide and benzimidazole fungicides in New Zealand vineyards. Plant Pathology, 38, 427–437.

    Article  Google Scholar 

  • Brent, K. J., & Hollomon, D. W. (1998). Fungicide resistance: The assessment of risk. Brussels, Belgium: Global Crop Protection Federation.

    Google Scholar 

  • Chapeland, F., Fritz, R., Lanen, C., Gredt, M., & Leroux, P. (1999). Inheritance and mechanisms of resistance to anilinopyrimidine fungicides in Botrytis cinerea (Botryotinia fuckeliana). Pesticide Biochemistry and Physiology, 64, 85–100.

    Article  CAS  Google Scholar 

  • Dianez, F., Santos, M., & Blanco, R. (2002). Fungicide resistance in Botrytis cinerea isolates from strawberry crops in Huelva (southwestern Spain). Phytoparasitica, 30, 529–534.

    Article  CAS  Google Scholar 

  • Elad, Y., Shabi, E., & Katan, T. (1988). Negative cross-resistance between benzimidazole and N-phenylcarbamate fungicides and control of Botrytis cinerea on grapes. Plant Pathology, 37, 141–147.

    Article  CAS  Google Scholar 

  • Faretra, F., & Pollastro, S. (1991). Genetic basis of resistance to benzimidazole and dicarboximide fungicides in Botryotinia fukeliana (Botrytis cinerea). Mycological Research, 95, 943–951.

    Article  CAS  Google Scholar 

  • Fritz, R., Lanen, C., Colas, V., & Leroux, P. (1997). Inhibition of methionine biosynthesis in Botrytis cinerea by the anilinopyrimidine fungicide pyrimethanil. Pesticide Science, 49, 40–46.

    Article  CAS  Google Scholar 

  • Hayashi, K., Schoonbeek, H., Sugiura, H., & DeWaard, M. (2001). Multidrug resistance in Botrytis cinerea associated with decreased accumulation of the azole fungicide oxpoconazole and increased transcription of the ABC transporter gene BcatrD. Pesticide Biochemistry and Physiology, 70, 168–179.

    Article  CAS  Google Scholar 

  • Jarvis, W. R. (1977). Botryotinia and Botrytis species - Taxonomy, physiology and pathogenicity. A guide to the literature, monograph no. 14. Ottawa, Canada: Department of Agriculture.

  • Kang, L. J., Zhang, X. F., Wang, W. Q., Ma, Z. Q., & Ma, Z. G. (2000). Estimation of fungicide-resistance and fitness of Botrytis cinerea. Chinese Journal of Pesticide Science, 2, 39–42.

    CAS  Google Scholar 

  • Katan, T. (1982). Resistance to 3, 5-dichlorophenyl-N-cyclic imide (‘dicarboximide’) fungicides in the grey mould pathogen Botrytis cinerea on protected crops. Plant Pathology, 31, 133–141.

    Article  CAS  Google Scholar 

  • Kato, T., Suzuki, K., Takahashi, J., & Kamoshita, K. (1984). Negatively correlated cross-resistance between benzimidazole fungicides and methyl A′-(3.5-dichlorophenyl) carbamate. Japanese Journal of Pesticide Science, 9, 489–495.

    CAS  Google Scholar 

  • Latorre, B. A., Flores, V., Sara, A., & Roco, A. (1994). Dicarboximide-resistant isolates of Botrytis cinerea from table grapes in Chile: survey and characterization. Plant Disease, 78, 990–994.

    CAS  Google Scholar 

  • Leroux, P., Chapeland, F., Desbrosses, D., & Gredt, M. (1999). Patterns of cross-resistance to fungicides in Botrytis cinerea isolates from French vineyards. Crop Protection, 18, 687–697.

    Article  CAS  Google Scholar 

  • Leroux, P., Fritz, R., Debieu, D., Albertini, C., Lanen, C., Bach, J., et al. (2002). Mechanisms of resistance to fungicides in field strains of Botrytis cinerea. Pest Management Science, 58, 876–888.

    Article  PubMed  CAS  Google Scholar 

  • Malatrakis, N. E. (1989). Resistance of Botrytis cinerea to dichlofluanid in greenhouse vegetables. Plant Disease, 73, 138–141.

    Article  Google Scholar 

  • Noethover, J., & Matteoni, J. A. (1986). Resistance of Botrytis cinerea to benomyl and iprodione in vineyards and greenhouses after exposure to the fungicides alone or mixed with captan. Plant Disease, 70, 398–402.

    Article  Google Scholar 

  • Pappas, A. C. (1997). Evolution of fungicide resistance in Botrytis cinerea in protected crops in Greece. Crop Protection, 16, 257–263.

    Article  CAS  Google Scholar 

  • Raposo, R., Delcan, K., Gomez, V., & Melgarrejo, P. (1996). Distribution and fitness of isolates of Botrytis cinerea with multiple fungicide resistance in Spanish greenhouses. Plant Pathology, 45, 497–505.

    Article  Google Scholar 

  • Rewal, N., Coley-Smith, J. R., & Sealy-Lewis, H. M. (1991). Studies on resistance to dichlofluanid and other fungicides in Botrytis cinerea. Plant Pathology, 40, 554–560.

    Article  CAS  Google Scholar 

  • Rosslenbroich, H. J., & Stuebler, D. (2000). Botrytis cinerea - history of chemical control and novel fungicides for its management. Crop Protection, 19, 557–561.

    Article  CAS  Google Scholar 

  • Yang, Y. T. (2003). Advance in research of epidemiology and control strategies for grey mould disease in China. Chinese Journal of Pesticide Science, 42, 6–9.

    Google Scholar 

  • Yuan, Z. H., Zhang, X. F., & Han, X. Y. (1996). Advances in fungicide-resistance research in Botrytis cinerea. Journal of Agricultural University of Hebei, 19, 107–111.

    Google Scholar 

  • Zhang, Y. J., Yan, X. Q., Han, J. C., & Liu, H. P. (2003). Detection of resistance to diethofencarb (NPC) in Botrytis cinerea Pers. Journal of Shanxi Agriculture University, 23, 308–311.

    Google Scholar 

  • Zhang, C. Q., Yuan, S. K., Sun, H. Y., Qi, Z. Q., Zhou, M. G., & Zhu, G. N. (2007). Sensitivity of Botrytis cinerea from vegetable greenhouses to boscalid. Plant Pathology, 56, 646–653.

    Article  CAS  Google Scholar 

  • Zhang, C. Q., Zhang, Y., & Zhu, G. N. (2008). The mixture of kresoxim-methyl and boscalid, an excellent alternative controlling grey mould caused by Botrytis cinerea. Annals of Applied Biology, 153, 205–213.

    Article  CAS  Google Scholar 

  • Zhou, M. G., Ye, Z. Y., & Liu, J. F. (1994). Advance in fungicide resistance in China. Journal of Nanjing Agricultural University, 17, 33–41.

    CAS  Google Scholar 

Download references

Acknowledgments

This research was partially supported by grant no. Y3080042 of Zhejiang Natural Science Foundation, grant no. 080104 of Zhejiang Undergraduate Innovation Project, and grant no. 2351000971 of Zhejiang Forest College funding. We sincerely thank Dr. S. K. Yuan of the Institute for the Control of Agrochemicals, Ministry of Agriculture (CAMA), P.R. China, for providing the technical grade fungicides.

Author information

Authors and Affiliations

  1. School of Agriculture and Food Science, Zhejiang Forest College, Hangzhou, China

    Chuan-qing Zhang

  2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China

    Chuan-qing Zhang, Jia-li Hu, Fang-ling Wei & Guo-nian Zhu

Authors
  1. Chuan-qing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jia-li Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fang-ling Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guo-nian Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chuan-qing Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Cq., Hu, Jl., Wei, Fl. et al. Evolution of resistance to different classes of fungicides in Botrytis cinerea from greenhouse vegetables in eastern China. Phytoparasitica 37, 351–359 (2009). https://doi.org/10.1007/s12600-009-0050-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12600-009-0050-7

Keywords

Search

Navigation