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Effects of dietary microalgal species and hormone treatment on the lorica size and reproductivity of heavy-ion beam irradiated rotifers

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  • Aquaculture
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Abstract

In aquatic seeding production, the feeding regime of fish larvae consists of rotifers to Artemia, while there is a lack of intermediate-sized feed in the range of 350–600 µm. To solve this problem, in our previous study, the euryhaline rotifer Brachionus plicatilis (L-type, Notojima strain) were irradiated with carbon heavy-ion-beams, and large-sized rotifer mutant lines (> 350 µm lorica length) were established. This study aimed to enhance the mutated morphometric characteristics and reproductivity through the combined use of three microalgal species (Nannochloropsis oculata, Tetraselmis tetrathele, and Chlorella vulgaris), and two hormones [juvenile hormone (JH) and gamma-aminobutyric acid (GABA)]. The combination of T. tetrathele and JH was effective in enhancing the mutated phenotype, and the largest lorica length of 344–425 µm was observed with no significant differences in population growth between the wild-type and mutant strains. The combination of N. oculata and GABA enhanced the population growth of the mutant line, and its growth rate was 2.4 times higher than that of the control group (without GABA). This study indicates that the morphometric characteristics and reproductivity of rotifer mutant lines could be regulated by feed and hormone supplementation. These findings can promote the efficient use of mutant rotifer lines in aquaculture facilities.

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Acknowledgements

This study was financially supported by the Japan Fisheries Agency (JFA) and JSPS KAKENHI grant numbers JP17H03862, JP20H03063, and JP20K21340 to A.H.. The authors would like to express our sincere gratitude to all the contributors improved the manuscript.

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Authors and Affiliations

  1. Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki, Japan

    Hee-Jin Kim, Kaichi Nakamura, Yoshitaka Sakakura, Koushirou Suga & Atsushi Hagiwara

  2. Organization for Marine Science and Technology, Nagasaki University, Nagasaki, Japan

    Yoshitaka Sakakura & Atsushi Hagiwara

  3. Nishina Center for Accelerator-Based Science, RIKEN, Wako, Japan

    Kazuhide Tsuneizumi, Tomoko Abe & Mieko Yamada

  4. Japan Fisheries Research and Education Agency, Japan Sea National Fisheries Research Institute, Miyazu, Japan

    Miki Kawada, Takashi Katayama & Nobuhiro Tezuka

  5. Japan Fisheries Research and Education Agency, National Research Institute of Fisheries Science, Yokohama, Japan

    Takanori Kobayashi

  6. Japan Fisheries Research and Education Agency, Seikai National Fisheries Research Institute, Ishigaki, Japan

    Masahiko Koiso

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  1. Hee-Jin Kim
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  2. Kaichi Nakamura
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  3. Yoshitaka Sakakura
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Correspondence to Hee-Jin Kim.

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Kim, HJ., Nakamura, K., Sakakura, Y. et al. Effects of dietary microalgal species and hormone treatment on the lorica size and reproductivity of heavy-ion beam irradiated rotifers. Fish Sci 89, 61–69 (2023). https://doi.org/10.1007/s12562-022-01652-8

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