Abstract
Gamma-aminobutyric acid (GABA) at 50 μg/ml and porcine growth hormone (GH) at 0.025 IU/ml were tested to see whether these chemicals would reduce the stress experienced by euryhaline rotifers Brachionus plicatilis species complex (L-, S- and SS-morphotypes) during low temperature (4–12°C) storage. Rotifers cultured at 25°C were transferred to 4–12°C for 10–30 days and transferred back to 25°C for recovery. GABA or GH were added to the rotifers at three different time points: 6 h before transfer from 25°C to low temperature (6h−), on day 7 after preservation at low temperature (7d+) and on the first day of recovery. For L-type rotifers, the GH treatment before the transfer to 4°C for 30 days was effective for better survival, while the GABA treatment was most effective for the S-type preserved at 10°C for 14 days. For the SS-type, the chemical treatments were not effective when the rotifers were preserved at 12°C for 14 days. After low temperature preservation, GABA treatments with the S- and SS-type rotifers just after their transfer to 25°C induced a relatively faster recovery of the rotifer population.
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This research was partly supported by the Ministry of Education, Science, Sports and Culture, Japan, Grant-in-Aid for Scientific Research (B) (2009–2011, No. 21380125).
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Assavaaree, M., Hagiwara, A. Effect of gamma-aminobutyric acid and porcine growth hormone on survival of the euryhaline rotifers Brachionus plicatilis sp. complex preserved at low temperature. Fish Sci 77, 599–605 (2011). https://doi.org/10.1007/s12562-011-0356-5
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DOI: https://doi.org/10.1007/s12562-011-0356-5