Abstract
Modori-inducing proteinases (MIPs) in horse mackerel and the effect of sodium citrate on the modori phenomenon were investigated. Cysteine protease caused the modori phenomenon only at 60 ℃, while metalloproteinase caused it at both 60 ℃ and 70 ℃. Moreover, the autolysis of myofibrillar proteins was mainly inhibited by ethylene diamine tetra-acetic acid (EDTA), verifying the existence of insoluble metalloproteinase. From the results comparing thermal gel properties and protease activities, we confirmed that increased activity of insoluble metalloproteinase corresponded to the weakening of the thermal gel. Thus, insoluble metalloproteinase was the major MIP in horse mackerel. Moreover, sodium citrate could markedly inhibit insoluble metalloproteinase activity at 60 ℃. The addition of sodium citrate significantly improved the breaking force of the thermal gel at 60 ℃ and 70 ℃, suggesting that sodium citrate could suppress the modori phenomenon by inhibiting insoluble metalloproteinase. Therefore, it is desirable to use sodium citrate in horse mackerel surimi-based products to prevent the modori phenomenon.
Similar content being viewed by others
References
An H, Weerasinghe V, Seymour TA, Morrissey MT (1994) Cathepsin degradation of pacific whiting surimi proteins. J Food Sci 59(5):1013–1017
CondoD, Herranz B, Borderías AJ, Moreno HM (2016) Different additives to enhance the gelation of surimi gel with reduced sodium content. Food Chem 196:791–799
Cao MJ, Osatomi K, Hara K, Ishihara T (2000) Identification of a myofibril-bound serine proteinase (MBSP) in the skeletal muscle of lizard fish Saurida wanieso which specifically cleaves the arginine site. Comp Biochem Physiol 125B:255–264
Edwards DR, Handsley MM, Pennington CJ (2008) The ADAM metalloproteinases. Mol Aspects Med 29(5):258–289
Geng J, Takahashi K, Toshiki K, Kasukawa M, Okazaki E, Osako K (2018) The effect of organic salts on the browning of dried squid products processed by air-drying. Food Chem 269:212–219
Haejung AN, Weerasinghe V, Seymour TA, Morrissey MT (1994) Cathepsin degradation of pacific whiting surimi proteins. J Food Sci 59(5):1013–1017
Hu YQ, Ji R, Jiang H, Zhang JJ, Chen JC, Ye XQ (2012) Participation of cathepsin L in modori phenomenon in carp (Cyprinus carpio) surimi gel. Food Chem 134(4):2014–2020
Kanzawa N, Yabuta H, Fujimi TJ, Tsuchiya T (2004) Solubility properties of a 65-kDa peptide prepared by restricted digestion of myosin with astacin-like squid metalloprotease. Zoolog Sci 21(2):159–162
Kanzawa N, Ogawa T, Asakura M, Okiyama K, Honda M, Tsuchiya T (2008) Comparative expression and tissue distribution analyses of astacin-like squid metalloprotease in squid and cuttlefish. Zoolog Sci 25(1):14–21
Klomklao S, Benjakul S, Kishimura H, Osako K, Simpson BK (2016) Trypsin inhibitor from yellowfin tuna (Thunnus albacores) roe: effects on gel properties of surimi from bigeye snapper (Priacanthus macracanthus). LWT-Food Sci Technol 65:122–127
Kudre T, Benjakul S, Kishimura H (2013) Effects of protein isolates from black bean and mungbean on proteolysis and gel properties of surimi from sardine (Sardinella albella). LWT Food Sci Technol 50(2):511–518
Kuwahara K, Konno K (2010) Suppression of thermal denaturation of myosin and salt-induced denaturation of actin by sodium citrate in carp (Cyprinus carpio). Food Chem 122(4):997–1002
Kuwahara K, Osako K, Okamoto A, Konno K (2006) Solubilization of myofibrils and inhibition of autolysis of squid mantle muscle by sodium citrate. J Food Sci 71(6):C358–C362
Kuwahara K, Osako K, Konno K (2004) Improved gel formation of Japanese common squid muscle as a result of suppression of autolysis by some organic salt. Nippon Suisan Gakkaishi 70(6):922–927
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lee J, Yuan P, Heidolph BB, Park JW (2018) Physicochemical properties of frozen Alaska Pollock fillets and surimi as affected by various sodium phosphates. J Food Process Preserv 42(3):e13530
Mueller JP, Liceaga AM (2016) Characterization and cryoprotection of invasive silver carp (Hypophthalmicthys Molitrix) protein hydrolysates. J Aquat Food Prod Technol 25:131–143
Matthew RF, Jae WP (2015) Salmon blood plasma: effective inhibitor of protease-laden Pacific whiting surimi and salmon mince. Food Chem 176(1):448–454
Noguchi H, Okazaki T, Minoshima R, Wakamiya E, Tsuji Y, Takano K (2016) Factors decreasing the gel-forming ability of threadfin bream surimi and effect of soybean whey addition. Food Preserv Sci 42(3):119–124
Osatomi K, Sasai H, Cao MJ, Hara K, Ishihara T (1997) Purification and characterization of myofibril-bound serine proteinase from carp Cyprinus carpio ordinary muscle. Comp Biochem Physiol 116B:183–190
Ohkubo M, Osatomi K, Hara K, Ishibashi T, Aranishi F (2005) A novel type of myofibril-bound serine proteinase from white croaker (Argyrosomus argentatus). Comp Biochem Physiol B 141:231–236
Park JW, Nozaki H, Suzuki T, Beliveau JL (2014) Historical review of surimi technology and market development. In: Park JW (ed) Surimi and surimi seafood. CRC Press, London, pp 3–24
Parvathy U, Sajan G (2014) Influence of cryoprotectant levels on storage stability of surimi from Nemipterus japonicus and quality of surimi-based products. J Food Sci Technol 51(5):982–987
Quan TH, Benjakul S (2017) Comparative study on the effect of duck and hen egg albumens on proteolysis and gel property of sardine surimi. Int J Food Prop 20(S3):S2786–S2797
Singh A, Benjakul S (2017) Effect of serine protease inhibitors from squid ovary on gel properties of surimi from Indian mackerel. J Texture Stud 48:541–549
Singh A, Benjakul S (2018) Proteolysis and its control using protease inhibitors in fish and fish products: a review. Comprehensive Rev Food Sci Food Safety 17:496–509
Snyman C, Niesler CU (2015) MMP-14 in skeletal muscle repair. J Muscle Res Cell Motil 36:215–225
Tamori J, Kanzawa N, Tajima T, Tamiya T, Tsuchiya T (1999) Purification and characterization of a novel isoform of myosinase from spear squid liver. J Biochem 126(5):969–974
Tan Y, Osatomi K, Nozaki Y, Ishihara T, Hara K (2006) Occurrence of two distinct molecular species of cathepsin B in carp Cyprinus carpio. Fish Sci 72(1):185–194
Tang SW, Feng GX, Gao RC, Ren JY, Zhou XD, Wang HY, Xu H, Zhao YH, Zeng MY (2019) Thermal gel degradation (Modori) in sturgeon (Acipenseridae) surimi gels. J Food Sci 84(12):3601–3607
Toyohara H (2008) Studies on fish muscle tenderization and improvement of fish meat quality by transgenic technology. Bullet Fish Res Agency 26(5):69–75
Ueki N, Wan J, Watabe S (2016a) Proteolytic profiles of walleye pollack (Theragra calcogramma) and white croaker (Pennahia argntata) meats in the presence of intestinal extracts from their own or different fish species. Food Sci Technol Res 22(6):787–792
Ueki N, Wan J, Watabe S (2016b) Deterioration of white croaker (Pennahia argentata) meat thermally-induced gel products caused by proteolytic enzymes in the contaminated intestine and kidney. Food Chem 199:416–422
Visessanguan W, Benjakul S, An H (2003) Purification and characterization of cathepsin L in arrowtooth flounder (Atheresthes stomias) muscle. Comp Biochem Physiol B: Biochem Mol Biol 134(3):477–487
Xu C, Wang C, Cai Q, Zhang Q, Weng L, Liu G, Su W, Cao M (2015) Matrix metalloproteinase 2 (MMP-2) plays a critical role in the softening of common carp muscle during chilled storage by degradation of type I and V collagens. J Agric Food Chem 63(51):10948–10956
Yokozawa Y, Tamai H, Tatewaki S, Tajima T, Tsuchiya T, Kanzawa N (2002) Cloning and biochemical characterization of astacin-like squid metalloprotease. J Biochem 132(5):751–758
Yamada K, Matsumiya M, Fukushima H (2020) Modori reaction in blue grenadier and Alaska pollock frozen surimi and myosin degradation behavior upon addition of protease inhibitors. CyTA-J Food 18(1):451–460
Yoshida A, Kirihara M, Ogata H, Cao M, Osatomi K, Hara K (2014) Proteolytic degradation of myofibrillar components by endogenous proteases in red sea bream muscle. Japanese J Food Chem 21(2):107–114
Yoshida A, Ohta M, Kuwahara K, Cao MJ, Hara K, Osatomi K (2015) Purification and characterization of cathepsin B from the muscle of horse mackerel trachurus japonicus. Mar Drugs 13(11):6550–6565
Yoshioka T, Kinoshita Y, Kato A, Kato S, Cho YJ, Konno K (2010) Preparation of heavy meromyosin from the autolyzed squid mantle muscle homogenate. Fish Sci 71(1):213–219
Yu NN, Xu YS, Jiang QX, Xia WS (2017) Textural and physicochemical properties of surimi gels prepared with potassium and calcium chloride as substitutes for sodium chloride. Int J Food Prop 20(S2):S1539–S1552
Zhou XX, Chen T, Lin HH, Chen H, Liu JH, Lyu F, Ding YT (2019) Physicochemical properties and microstructure of surimi treated with egg white modified by tea polyphenols. Food Hydrocolloids 90:82–89
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Jiang, YR., Yoshida, A., Ohta, M. et al. Quality improvement of the thermal gel of horse mackerel by sodium citrate and its inhibition of the insoluble metalloproteinase. Fish Sci 89, 93–100 (2023). https://doi.org/10.1007/s12562-022-01651-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12562-022-01651-9