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Antioxidant and anti-hyaluronidase activities of taurine-enriched enzymatic hydrolysates prepared from Turbo cornutus, a by-product of fish processing in Jeju Island

Abstract

Turbo cornutus is an edible sea snail species whose viscera parts are by-products of fish processing. Although they can be used for human consumption as good sources of functional ingredients, they are generally discarded. The objective of the present study was to prepare bioactive hydrolysate (MSVAH) from viscera parts of T. cornutus using enzymatic hydrolysis, and to investigate their antioxidant and anti-hyaluronidase properties for use as potential functional food ingredients. In MSVAH, taurine was found to be the most abundant. Taurine is known to have excellent physiological activity. Taurine-enriched MSVAH showed good capacities of DPPH, peroxyl radical scavenging, and hyaluronidase inhibition, with IC50 values of 0.50 mg/mL, 0.32 mg/mL, and 2.87 mg/mL, respectively. MSVAH also significantly improved cell viability and inhibited intracellular reactive oxygen species (ROS) dose-dependently against oxidative stress induced by 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH), a free-radical-generating compound, in immortalized human keratinocytes (HaCaT cells). Furthermore, MSVAH upregulated hyaluronic acid (HA)-synthesizing enzyme hyaluronan synthase 2 (HAS2) expression level, but downregulated expression level of HA-degrading enzyme hyaluronidase 2 (HYAL2), resulting in increased HA production in keratinocytes. Taken together, these results suggest that taurine-enriched MSVAH derived from viscera parts of T. cornutus, known to be by-products of fish processing, could be used to prepare potential bioactive hydrolysates for developing functional foods and nutraceutical products.

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Acknowledgements

This research was funded by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the “Regional Specialized Industry Development Plus Program (R&D, S3083328)” supervised by the Korea Institute for Advancement of Technology (KIAT) and research grants from the Korea Institute of Ocean Science and Technology (PEA0022). It was also supported by Soonchunhyang University Research Fund.

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Correspondence to Soo-Jin Heo or Seung-Hong Lee.

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Im, S.T., Kang, N., Kim, J. et al. Antioxidant and anti-hyaluronidase activities of taurine-enriched enzymatic hydrolysates prepared from Turbo cornutus, a by-product of fish processing in Jeju Island. Fish Sci 88, 509–517 (2022). https://doi.org/10.1007/s12562-022-01605-1

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Keywords

  • Turbo cornutus
  • Viscera
  • Enzymatic hydrolysis
  • Antioxidant and anti-hyaluronidase
  • Functional food ingredients