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Comparison of resting egg gene expression with different hatchability related to salinity variations in the marine rotifer Brachionus manjavacas

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Abstract

Salinity is a significant factor in affecting resting egg hatching in the euryhaline rotifers. In order to clarify the effects of salinity on resting egg hatchability, this study investigated gene expressions of resting eggs subjected to two different incubation salinities (at 17 and 33 ppt) in the rotifer Brachionus manjavacas. The resting eggs formed at 17 ppt showed a higher hatching rate at 17 ppt incubation salinity, compared to those at 33 ppt. Related to these circumstances, the resting eggs incubated at 17 ppt expressed genes which have putative functions implying cellular differentiation and embryonic development: late embryogenesis abundant protein (LEAs-1), α-amylase, and deaminase. The resting eggs incubated at 33 ppt highly expressed the genes related to the environmental stresses: AP2 transcription factors (AP2TF), and ATP decomposition: ABC transporter permease (ABC-TP), NAD + synthase, copper-translocating P-type ATPase (CTP-ATPase). It is expected that the resting eggs incubated at 33 ppt may need more energy (ATP) to endure saline stress during incubation. The obtained results indicated that the resting eggs regulate their hatching by allocating energy between embryo development and self-defense against environmental conditions like salinity stresses.

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Acknowledgements

This research was supported by JSPS KAKENHI Grant Number JP17H03862 and 15K14791 to Atsushi Hagiwara. The authors deeply appreciate the comments and suggestions from Dr. Helen Marcial and the anonymous reviewers who greatly improved the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China

    Chengyan Han & Mingyou Li

  2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China

    Chengyan Han & Mingyou Li

  3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China

    Chengyan Han & Mingyou Li

  4. Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Bunkyo 1-14, Nagasaki, 852-8521, Japan

    Hee-Jin Kim, Koushirou Suga & Atsushi Hagiwara

  5. Organization for Marine Science and Technology, Nagasaki University, Bunkyo 1-14, Nagasaki, 852-8521, Japan

    Koushirou Suga & Atsushi Hagiwara

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  1. Chengyan Han
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  2. Hee-Jin Kim
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  3. Koushirou Suga
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  4. Mingyou Li
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  5. Atsushi Hagiwara
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Correspondence to Hee-Jin Kim.

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Han, C., Kim, HJ., Suga, K. et al. Comparison of resting egg gene expression with different hatchability related to salinity variations in the marine rotifer Brachionus manjavacas. Fish Sci 84, 663–669 (2018). https://doi.org/10.1007/s12562-018-1213-6

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  • DOI: https://doi.org/10.1007/s12562-018-1213-6

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