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Poly(3-hydroxybutyrate) production using mannitol as a sole carbon source by Burkholderia sp. AIU M5M02 isolated from a marine environment

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  • Chemistry and Biochemistry
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Abstract

We isolated Burkholderia sp. AIU M5M02, which accumulates poly(3-hydroxybutyrate) [P(3HB)] homopolymer in a nitrogen-limiting mineral salt medium containing mannitol as a sole carbon source, from a marine environment. When the isolated strain was cultured in a nitrogen-limiting mineral salt medium (pH 5.0) containing 10% (v/v) mannitol as a sole carbon source for 4 days, P(3HB) content and P(3HB) productivity reached 40 weight percent and 1.5 g/l, respectively. Thus, we demonstrated that Burkholderia sp. AIU M5M02 can be utilized for P(3HB) production from mannitol, which is a major sugar in seaweed. Furthermore, the isolate utilized not only mannitol but also gluconic acid, glycerol, starch, and sugars such as fructose, glucose, maltose, sucrose, and xylose to produce P(3HB). Based on these results, we expect to be able to apply Burkholderia sp. AIU M5M02 to the production of P(3HB) from other raw materials with low environmental loads.

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Fig. 1a–d

Reproduced with permission from Yamada et al.

Fig. 2

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Fig. 3a, b

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Fig. 4

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Fig. 5

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Fig. 6

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Acknowledgements

We thank Mr. Kimiaki Naiki (Iwate Fisheries Technology Center) for sampling of the shallow sea mud from Ofunato Bay. The work described here was supported by the Regional Innovation R&D Program (to M. Yamada) of Iwate Prefecture, Japan.

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Authors and Affiliations

  1. Department of Biological Chemistry and Food Science, Iwate University, Ueda-3, Morioka, 020-8550, Japan

    Miwa Yamada, Ai Yukita, Yuta Hanazumi, Yuki Yamahata, Hiroki Moriya, Masao Miyazaki, Tetsuro Yamashita & Hitoshi Shimoi

  2. Education and Research On Sanriku Fishery Industry Department, Organization for Revitalization of the Sanriku Region and Regional Development, Iwate University, Ueda-3, Morioka, 020-8550, Japan

    Miwa Yamada & Tetsuro Yamashita

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  1. Miwa Yamada
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  2. Ai Yukita
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Correspondence to Miwa Yamada.

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Yamada, M., Yukita, A., Hanazumi, Y. et al. Poly(3-hydroxybutyrate) production using mannitol as a sole carbon source by Burkholderia sp. AIU M5M02 isolated from a marine environment. Fish Sci 84, 405–412 (2018). https://doi.org/10.1007/s12562-017-1164-3

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