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Pathogenicity comparison of high- and low-virulence strains of Vibrio scophthalmi in olive flounder Paralichthys olivaceus

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  • Aquaculture
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Abstract

Vibrio scophthalmi, a bacterial pathogen of olive flounder Paralichthys olivaceus, exhibits strain-dependent virulence. No information is available on the comparative pathogenicity of different strains of V. scophthalmi toward olive flounder. In this study, high- and low-virulence strains (HVS and LVS, respectively) were compared in terms of their pathogenic characteristics, including adhesion and survival, superoxide dismutase (SOD) activity, and extracellular products (ECP) of bacterial cells. The cell-mediated defense of macrophages from olive flounder against V. scophthalmi infection in vitro was also investigated. The results demonstrated that the SOD activity of the HVS was higher than that of the LVS. The number of viable cells of the HVS in serum increased by two log units after 18 h, whereas that of the LVS decreased. The number of cells of the HVS in skin mucus increased significantly while that of the LVS remained constant. The LD50 values of the HVS and LVS ECP toward olive flounder were 10.14 and 15.99 μg protein/g fish, respectively. The ECP were positive for naphthol-AS-BI-phosphohydrolase, lipase, gelatinase, and leucine arylamidase. The extracellular O2 − overflow and intracellular O2 − concentration of macrophages induced by the HVS were lower than those induced by the LVS. Significantly more nitric oxide was produced by the HVS than by the LVS.

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Authors and Affiliations

  1. Department of Aquatic Life Medicine, Pukyong National University, Busan, 608737, Republic of Korea

    Guo Qiao & Soo Il Park

  2. West Sea Fisheries Research Institute, National Fisheries Research & Development Institute (NFRDI), Incheon, 400420, Republic of Korea

    Guo Qiao & In-Kwon Jang

  3. Southwest Sea Fisheries Research Institute, NFRDI, Yeosu, 555823, Republic of Korea

    Kyoung Mi Won

  4. Institute of Fisheries Sciences, Pukyong National University, 259, Dongbaeck-Ri, Ilkwang-Myun, Gijang-gun, Busan, 619911, Republic of Korea

    Sung Ho Woo

  5. Aquatic Animal Health Research Unit, US Department of Agriculture, 990 Wire Road, Auburn, AL, 36832, USA

    De-Hai Xu

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  1. Guo Qiao
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  2. In-Kwon Jang
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Correspondence to Soo Il Park.

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Qiao, G., Jang, IK., Won, K.M. et al. Pathogenicity comparison of high- and low-virulence strains of Vibrio scophthalmi in olive flounder Paralichthys olivaceus . Fish Sci 79, 99–109 (2013). https://doi.org/10.1007/s12562-012-0567-4

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