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Microbiological characteristics of Vibrio scophthalmi isolates from diseased olive flounder Paralichthys olivaceus

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  • Aquaculture
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Abstract

In 2005, massive mortality occurred in olive flounder Paralichthys olivaceus farms in Korea, and five isolates were collected from diseased fish. In this study, microbiological and pathogenic characteristics of these isolates were studied. The isolates gave negative results in lysine and ornithine decarboxylase, ortho-nitrophenyl-β-galactoside, and citrate tests, and positive results in urease, esculinase, and nitrate reduction tests. The isolates produced acid from adipate, fructose, d-glucose, and maltose, and gave positive results in alkaline phosphatase, esterase lipase, leucine arylamidase, and naphthol-AS-BI-phosphohydrolase. According to genetic analysis, 16S rRNA gene sequences showed 98–100 % identity with both Vibrio scophthalmi and V. ichthyoenteri. The dnaJ gene sequences presented a higher identity with V. scophthalmi than with V. ichthyoenteri. Thus, the isolates were identified as V. scophthalmi. Pathogenicity of the five isolates in olive flounder was different and LD50 values were from 106 to 108 CFU/g fish. Symptoms included darkening of skin, hemorrhage of liver and intestine, ascites, and distended abdomen. Histopathological changes included hemopoiesis dilatation and epithelial hyaline droplets in kidney, macrophage infiltration and ellipsoid dilatation in spleen, vascular dilatation, submucosal edema, and serosa inflammation of intestine. Cumulative mortality was 25 % for fish singly infected by isolate A19008 or Streptococcus parauberis, and increased to 87.5 % in super-infection group with these two pathogens.

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Authors and Affiliations

  1. Department of Aquatic Life Medicine, Pukyong National University, Busan, 608737, Republic of Korea

    Guo Qiao, Sung Ho Woo & Soo Il Park

  2. Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute (NFRDI), Tongyeong, 650-943, Republic of Korea

    Deok Chan Lee

  3. Key Laboratory of Mariculture and Biotechnology, Agriculture Ministry, PRC, Dalian Ocean University, Dalian, 116023, Liaoning, China

    Hua Li

  4. US Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, Auburn, AL, 36832, USA

    De-Hai Xu

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  1. Guo Qiao
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Correspondence to Soo Il Park.

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Qiao, G., Lee, D.C., Woo, S.H. et al. Microbiological characteristics of Vibrio scophthalmi isolates from diseased olive flounder Paralichthys olivaceus . Fish Sci 78, 853–863 (2012). https://doi.org/10.1007/s12562-012-0502-8

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