Abstract
Fish species identification techniques for authentic food labeling were developed using species-specific PCR primers for cod roe products. A salted, seasoned fish roe product, karashimentaiko (chilli cod roe), is produced from the eggs of Alaska pollock, Theragra chalcogramma, according to the fair trade competition agreement authorized by the Fair Trade Commission of the Japanese government. To examine whether Alaska pollock ovaries or those of other fish species are being used as raw materials for the fish roe products, we developed species identification techniques using PCR amplification of a 255-bp fragment encoding the mitochondrial ATP synthase Fo subunit 6 (ATP6) gene with a species-specific primer set for Alaska pollock mitochondrial DNA. We also designed two species-specific primer sets corresponding to the mitochondrial ATP6 and cytochrome b (cytb) for Gadus spp. and Micromesistius spp. by PCR amplification of 332- and 223-bp fragments, respectively. We examined the species specificity of these PCR-based methods among nine commercially important Gadidae species.
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References
Mafra M, Ferreira IMPLVO, Oliveira MBPP (2008) Food authentication by PCR-based methods. Eur Food Res Technol 227:649–665
Yamashita M, Namikoshi A, Iguchi J, Takashima Y, Hossain MA, Yabu T, Yamashita Y (2008) Molecular identification of species and the geographic origin of seafood. In: Tsukamoto K, Kawamura T, Takeuchi T, Beard TD Jr, Kaiser MJ (eds) Fisheries for global welfare and environment. Memorial book of the 5th World Fisheries Congress. Terrapub, Tokyo, pp 279–306
Yanagimoto T, Kitamura T (2002) Identification of three gadoid species around Hokkaido using the mitochondorial cytochrome b gene (in Japanese). Nippon Suisan Gakkaishi 68:893–899
Calo-Mata P, Sotelo CG, Pérez-Martin RI, Rehbein H, Hold GL, Russell VJ, Pryde S, Quinteiro J, Rey-Mendez M, Rosa C, Santos AT (2003) Identification of gadoid fish species using DNA-based techniques. Eur Food Res Technol 217:259–264
Aranishi F, Okimoto T, Izumi S (2005) Identification of gadoid (Pisces, Gadidae) by PCR-RFLP analysis. J Appl Genet 46:69–73
Akasaki T, Yanagimoto T, Yamakami K, Tomonaga H, Sato S (2006) Species identification and PCR-RFLP analysis of cytochrome b gene in cod fish (Order Gadiformes) products. J Food Sci 71:190–195
Pérez M, Presa P (2008) Validation of a tRNA-Glu-cytochrome b key for the molecular identification of 12 hake species (Merluccius spp.) and Atlantic cod (Gadus morhua) using PCR-RFLPs, FINE, and BLAST. J Agric Food Chem 56:10865–10871
Yanagimoto T, Kitamura T, Kobayashi T (2008) Population structure of walleye pollock inferred by DNA analysis (in Japanese). DNA Polymorph 16:135–139
Miya M, Nishida M (2000) Use of mitogenomic information in teleostean molecular phylogenetics: a tree-based exploration under the maximum-parsimony optimality criterion. Mol Phylogenet Evol 17:437–455
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
Yanagimoto T, Kitamura T, Kobayashi T (2004) Complete nucleotide sequence and variation of mitochondrial DNA from 10 individuals of walleye pollock, Theragra chalcogramma. Fish Sci 70:885–895
Johansen S, Bakkle I (1996) The complete mitochondrial DNA sequence of Atlantic cod (Gadus morhua): relevance to taxonomic studies among codfishes. Mol Mar Biol Biotechnol 3:203–214
Coulson MW, Marshall HD, Pepin P, Carr SM (2006) Mitochondrial genomics of gadine fishes: implications for taxonomy and biogeographic origins from whole-genome data set. Genome 49:1115–1130
Taylor MI, Fox C, Rico I, Rico C (2002) Species-specific TaqMan probe for simultaneous identification of Atlantic cod (Gadus morhua L.), haddock (Melanogrammus aeglefinus L.) and whiting (Merlagius merlangus L.). Mol Ecol Notes 2:599–601
Ursvik A, Breines R, Christiansen JS, Fevolden SE, Coucheron DH, Johansen SD (2007) A mitogenomic approach to the taxonomy of pollocks: Theragra chalcogramma and T. finnmarchica represent one single species. BMC Evol Biol 7:86
Carr SM, Marshall HD (2008) Intraspecific phylogeographic genomics from multiple complete mtDNA genomes in Atlantic cod (Gadus morhua): origins of the “codmother” transatlantic vicariance and midglacial population expansion. Genetics 180:381–389
Bakke I, Johansen SD (2005) Molecular phylogenetics of Gadidae and related Gadiformes based on mitochondrial DNA sequences. Mar Biotechnol 7:61–69
Teletchea F, Laudet V, Hänni C (2006) Phylogeny of the Gadidae (sensu Svetovidov, 1948) based on their morphology and two mitochondrial genes. Mol Phylogenet Evol 38:189–199
Dooley JJ, Sage HD, Clarke M-AL, Brown HM, Garrett SD (2005) Fish species identification using PCR-RFLP analysis and Lab-on-a-Chip capillary electrophoresis: application to detect white fish species in food products and an interlaboratory study. J Agric Food Chem 53:3348–3357
Hird HJ, Hold GL, Chisholm J, Reece P, Russell VJ, Brown J, Goodier R, MacArthur R (2005) Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR. Eur Food Res Technol 220:633–637
Acknowledgments
This study was conducted as part of the development of evaluation and management methods for supply of safe, reliable, and functional food and farm produce. It was funded by the National Food Research Institute and the Ministry of Agriculture, Forestry, and Fisheries of Japan. We thank Dr. Iciar Martinez and Nippon Suisan Kaisha, Ltd., for providing fish samples.
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Namikoshi, A., Takashima, Y., Iguchi, J. et al. Species identification of Alaska pollock, Gadus spp., and Micromesistius spp. in cod roe products using a PCR-based method. Fish Sci 77, 671–678 (2011). https://doi.org/10.1007/s12562-011-0349-4
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DOI: https://doi.org/10.1007/s12562-011-0349-4