Abstract
Overall quality attributes between deskinned Asian seabass and mangrove red snapper fillet are similar. This may lead to the substitution of Asian seabass, a cheaper species, with the latter. Therefore, the authentication method for distinguishing these two species could conquer adulteration. Four primers were designed, based on mitochondrial cytb gene alignment. Polymerase chain reaction (PCR) was performed using the DNA of both species with 35 cycles of amplification. The primers provided the amplicons with the size of 480, 396, 185, and 268 for primers 1, 2, 3, and 4, respectively. All primers successfully differentiated Asian seabass from mangrove red snapper both raw and cooked fillets from at least 30 mg of the sample weight. Moreover, primer 4 showed the highest specificity when tested with other 5 species of fish generally sold in the Thai market. The limit of detection was around 6 ng of Asian seabass DNA in a total of 120 ng mangrove red snapper DNA. Therefore, the PCR-based method using primer 4 was a useful tool for the identification of Asian seabass adulterated in mangrove red snapper fillets or slices. This primer could be implemented for authentication of fish and fish products to prevent mislabeling for customers.
Similar content being viewed by others
References
T. Van Holt, W. Weisman, S. Käll, B. Crona, R. Vergara, Ann. N Y Acad. Sci. 1421, 46 (2018)
C.F. Staffen, M.D. Staffen, M.L. Becker, S.E. Löfgren, Y.C.N. Muniz, R.H.A. de Freitas, A.R. Marrero, PeerJ 5, e4006 (2017)
J. Helgoe, K.J. Oswald, J.M. Quattro, Fish. Res. 222, 105400 (2020)
X. Xiong, L. Yao, X. Ying, L. Lu, L. Guardone, A. Armani, A. Guidi, X. Xiong, Food Control 88, 123 (2018)
A. Munguia-Vega, R. Terrazas-Tapia, J.F. Dominguez-Contreras, M. Reyna-Fabian, P. Zapata-Morales, PLOS ONE 17, e0265960 (2022)
K. Kroetz, G.M. Luque, J.A. Gephart, S.L. Jardine, P. Lee, K. Chicojay Moore, C. Cole, A. Steinkruger, C.J. Donlan, Proc. Natl. Acad. Sci. U S A 117, 30318 (2020)
A.M. Peterson, G.E. McBride, S.K. Jhita, R.S. Hellberg, Heliyon 7, e06713 (2021)
R.S. Hellberg, K. Everstine, S.A. Sklare, Food Fraud: A Global Threat with Public Health and Economic Consequences, 1st edn. (Academic Press, Cambridge, 2020)
T.P. Piddocke, G.L. Butler, P.A. Butcher, J. Stewart, D.J. Bucher, L. Christidis, J. Fish. Biol. 86, 1587 (2015)
M. Nurliyana, B. Lukman, M.Y. Ina-Salwany, M. Zamri-Saad, S. Annas, H.T. Dong, C. Rodkhum, M.N.A. Amal, Aquaculture 528, 735600 (2020)
L. Orbán, X. Shen, N. Phua, L. Varga, Front. Genet. 12, 506754 (2021)
N.A. Robinson, G. Schipp, J. Bosmans, D.R. Jerry, Aquac. Res. 41, e643 (2010)
Barramundi - aquaculture prospects. (NSW Department of Primary Industries, 2022), https://www.dpi.nsw.gov.au/fishing/aquaculture/publications/species-freshwater/barramundi-aquaculture-prospects. Accessed 13 February 2022
T.J.R. Fernandes, J.S. Amaral, I. Mafra, Crit. Rev. Food Sci. Nutr. 61, 3904 (2021)
K. Böhme, P. Calo-Mata, J. Barros-Velázquez, I. Ortea, J. Agric. Food Chem. 67, 3854 (2019)
A.J. Silva, R.S. Hellberg, Advances in Food and Nutrition Research (Academic Press, 2021), pp. 207–255
T.J.R. Fernandes, J. Costa, M.B.P.P. Oliveira, I. Mafra, Food Chem. 230, 49 (2017)
V. Hrbek, K. Zdenkova, D. Jilkova, E. Cermakova, M. Jiru, K. Demnerova, J. Pulkrabova, J. Hajslova, Foods 9, 1269 (2020)
Y. Zhang, Q. Qu, M. Rao, N. Zhang, Y. Zhao, F. Tao, Sci. Rep. 10, 8965 (2020)
F. Sievers, D.G. Higgins, Protein Sci. 27, 135 (2018)
K. Gopi, D. Mazumder, J. Sammut, N. Saintilan, Trends Food Sci. Technol. 91, 294 (2019)
C.M. Franco, R.L. Ambrosio, A. Cepeda, A. Anastasio, Curr. Opin. Food Sci. 42, 86 (2021)
A. Cutarelli, G. Galiero, F. Capuano, F. Corrado, Food and Nutrition Sciences 9, 369 (2018)
D. Dwiyitno, S. Hoffman, K. Parmentier, C.V. Keer, Biodiversitas Journal of Biological Diversity 23, (2022)
Y.-M. Lee, S. Lee, H.-Y. Kim, Foods 10, 2631 (2021)
M.Z. Ghouri, M. Ismail, M.A. Javed, S.H. Khan, N. Munawar, A.B. Umar, Mehr-un-Nisa, S.O. Aftab, S. Amin, Z. Khan, A. Ahmad, Frontiers in Marine Science 7, (2020)
J. Quinteiro, C.G. Sotelo, H. Rehbein, S.E. Pryde, I. Medina, R.I. Pérez-Martín, M. Rey-Méndez, I.M. Mackie, J. Agric. Food Chem. 46, 1662 (1998)
A. Komissarov, S. Vij, A. Yurchenko, V. Trifonov, N. Thevasagayam, J. Saju, P.S.R. Sridatta, K. Purushothaman, A. Graphodatsky, L. Orbán, I. Kuznetsova, Genes (Basel) 9, 464 (2018)
R.D. Ward, B.H. Holmes, G.K. Yearsley, J. Fish Biol. 72, 458 (2008)
S. Vij, K. Purushothaman, G. Gopikrishna, D. Lau, J.M. Saju, K.V. Shamsudheen, K.V. Kumar, V.S. Basheer, A. Gopalakrishnan, M.S. Hossain, S. Sivasubbu, V. Scaria, J.K. Jena, A.G. Ponniah, L. Orbán, Frontiers in Marine Science 1, (2014)
N.-Y.-S. Kim, E.-J. Park, S.-H. Lee, K.-H. Mun, J.-Y. Yang, J.-B. Kim, Food Sci. Biotechnol. 30, 497 (2021)
E.N. Hulley, S. Tharmalingam, A. Zarnke, D.R. Boreham, PLoS One 14, e0210165 (2019)
M. Quintrel, F. Jilberto, M. Sepúlveda, M.E. Marín, D. Véliz, C. Araneda, M.A. Larraín, Foods 10, 1684 (2021)
Acknowledgements
The authors would like to thank the International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University for the use of all facilities. Prachayacharn scholarship (AGR6502111N) from Prince of Songkla University was acknowledged.
Funding
This study was supported in part by The International Research Network program from the National Research Council of Thailand (NRCT), and Chair Professor Grant (grant no: P-20-52297).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Ethics approval
Not applicable.
Consent to participate
Not applicable.
Consent for publication
All authors have read and approved the final manuscript.
Conflict of interest
The authors hereby declare to have no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Saetang, J., Benjakul, S. Identification and differentiation of Asian seabass and mangrove red snapper fillets by CYTB sequence-based PCR analysis. Food Measure 16, 4596–4601 (2022). https://doi.org/10.1007/s11694-022-01545-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-022-01545-5