Abstract
Aspergillus fresenii and Aspergillus sulphureus produce ochratoxin A (OTA), which is a secondary metabolite of Aspergillus and Penicillium species, with nephrotoxic effects and potential carcinogenic activity. The aim of this study was to determine the effects of temperature (20, 30, and 37 °C), water activity (0.82, 0.86, 0.90, 0.94, and 0.98 aw), incubation period (5, 10, 15, and 30 days) on fungal growth, and OTA production by A. fresenii and A. sulphureus on Nyjer oil seeds. There was no fungal growth at 0.82 aw. The two fungal species were able to produce OTA from the fifth day of incubation from 0.86 to 0.98 aw and temperature 20 to 37 °C. Aspergillus fresenii produced the highest concentration of OTA (643 μg/kg) at 0.90 aw and 37 °C within 15 days, while A. sulphureus produced the highest level of OTA (724 μg/kg) at 0.98 aw and 20 °C within 10 days. The optimum water activity and temperature for the growth of both fungi were similar at 0.94 aw and at 30 °C. There was statistically significant difference between the levels of OTA production among the two fungi. Overall, A. sulphureus produced significantly higher levels of OTA (p < 0.05). Higher temperature (37 °C) and 0.90–0.94 aw were optimal for OTA production by A. fresenii. Our results show that Nyjer seeds can support the growth of A. fresenii and A. sulphureus and OTA production, and the two species had similar temperature and water activity requirements for growth but different requirements for OTA production on Nyjer seeds.
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References
Adebajo O, Idowu A, Adesanya O (1994) Mycoflora, and mycotoxins production in Nigerian corn and corn-based snacks. Mycopathologia 126:183–192
Alborch L, Bragulat MR, Abarca ML, Cabanes FJ (2011) Effect of water activity, temperature and incubation time on growth and ochratoxin A production by Aspergillus niger and Aspergillus carbonarius on maize kernels. Int J Food Microbiol 147:53–57
Ayalew A, Fehrmann H, Lepschy J, Beck R, Abate D (2006) Natural occurrence of mycotoxins in staple cereals from Ethiopia. Mycopathologia 162:57–63
Bragulat MR, Abarca ML, Cabanes FJ (2001) An easy screening method for fungi producing ochratoxin A in pure culture. Int J Food Microbiol 71:139–144
Commission of the European Communities (2006) Commission regulation (EC) no. 401/2006 of 2006 February 23. Laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. J Eur Union 70:12–30
Das A, Paul T, Halder SK, Jana A, Maity C, Das Mohapatra PK, Pati BR, Mondal KC (2013) Production of cellulolytic enzymes by Aspergillus fumigatus ABK9 in wheat bran-rice straw mixed substrate and use of cocktail enzymes for deinking of waste office paper pulp. Bioresour Technol 128:290–296
Denli M, Perez JF (2010) Ochratoxins in feed, a risk for animal and human health: control strategies. Toxins (Basel) 2:1065–1077
Dohoo IR, Martin SW, Stryhn H (2009) Veterinary epidemiologic research, 2nd edn. VER, Inc., Charlottetown
Drunday V, Pacin A (2013) Occurrence of ochratoxin A in coffee beans, ground roasted coffee and soluble coffee and method validation. Food Control 30:675–678
Food and Agriculture Organization (2004) Food and Agriculture Organization (FAO) food and nutrition papers 81: worldwide regulations for mycotoxins in food and feed in 2003. FAO, Rome
Gizachew D, Szonyi B, Tegegne A, Hanson J, Grace D (2016) Aflatoxin contamination of milk and dairy feeds in the Greater Addis Ababa milk shed, Ethiopia. Food Control 59:773–779
Ibáñez-Vea M, Martinez R, González-Peñas E, Lizarraga E, de Cerain AL (2011) Co-occurrence of aflatoxins, ochratoxin A and zearalenone in breakfast cereals from Spanish market. Food Control 22:1949–1955
Kumagai S, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Aoyama K, Fujita K, Kai S, Sato T, Saito S, Yoshiike N, Sugita-Konishi Y (2008) Aflatoxin and ochratoxin A contamination of retail foods and intake of these mycotoxins in Japan. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:1101–1106
Lin LC, Chen PC, Fu YM, Shih DYC (2005) Ochratoxin A contamination in coffees, cereals, red wines and beers in Taiwan. Food Drug Anal 13:84–92
Madhyastha SM, Marquardt RR, Frohlich AA, Platford G, Abramson D (1990) Effects of different cereal and oilseed substrates on the growth and production of toxins by Aspergillus alutaceus and Penicillium verrucosum. J Agric Food Chem 38:1506–1510
Marin S, Ramos AJ, Cano-Sancho G, Sanchis V (2013) Mycotoxins: occurrence, toxicology, and exposure assessment. Food Chem Toxicol 60:218–237
Nguyen MT, Tozlovanu M, Tran TL, Pfohl-Leszkowicz A (2007) Occurrence of aflatoxin B1, citrinin and ochratoxin A in rice in five provinces of the central region of Vietnam. Food Chem 105:42–47
Northolt MD, Van Egmond HP, Paulsch WE (1979) Ochratoxin A production by some fungal species in relation to water activity and temperature. J Food Prot 42:485–490
Otteneder H, Majerus P (2000) Occurrence of ochratoxin A (OTA) in wines: influence of the type of wine and its geographical origin. Food Addit Contam 17:793–798
Palumbo JD, O'Keeffe TL (2015) Detection and discrimination of four Aspergillus section Nigri species by PCR. Lett Appl Microbiol 60:188–195
Pardo E, Marin S, Sanchis V, Ramos AJ (2004) Prediction of fungal growth and ochratoxin A production by Aspergillus ochraceus on irradiated barley grain as influenced by temperature and water activity. Int J Food Microbiol 95:79–88
Peters J, van Dam R, van Doorn R, Katerere D, Berthiller F, Haasnoot W, Nielen MWF (2017) Mycotoxin profiling of 1000 beer samples with a special focus on craft beer. PLoS One 12:e0185887
Reddy L, Bhoola K (2010) Ochratoxins—food contaminants: impact on human health. Toxins (Basel) 2:771–779
Schatzmayr G, Streit E (2013) Global occurrence of mycotoxins in the food and feed chain: facts and figures. World Mycotoxin J 6:213–222
Schnaufer R (2012) Ochratoxin A in oil seeds and nuts. http://www.cvuas.de/pub/beitrag_printversion.asp?ID=1666. Accessed April 6 2018
Smith MC, Madec S, Coton E, Hymery N (2016) Natural co-occurrence of mycotoxins in foods and feeds and their in vitro combined toxicological effects. Toxins (Basel) 8:94
Teren J, Varga J, Hamari Z, Rinyu E, Kevei F (1996) Immunochemical detection of ochratoxin A in black Aspergillus strains. Mycopathologia 134:171–176
University of British Columbia (n.d.) What is Noug? www3.botany.ubc.ca/noug Accessed April 6 2018
Vega M, Munoz K, Aranda SC, Aranda M, Campos V, Villegas R, Villarroel O (2009) Solidphase extraction and HPLC determination of ochratoxin A in cereals products on Chilean market. Food Control 20:631–634
Wearing AH, Burgess LW (1979) Water potential and the saprophytic growth of Fusarium roseum ‘Graminearum’. Soil Biol Biochem 11:661–667
Windingstad RM, Cole RJ, Nelson PE, Roffe TJ, George RR, Dorner JW (1989) Fusarium mycotoxins from peanuts suspected as a cause of sandhill crane mortality. J Wildl Dis 25:38–46
Wobeser GA (1997) Mycotoxins, in diseases of wild waterfowl, 2nd edn. Plenum Press, New York
Acknowledgements
We thank Jessica Orr for her valuable assistance in the laboratory. We also thank Susan Delatorre for ordering the various laboratory items required for the research.
Funding
This study is supported with a graduate research assistantship to Yung-Chen Hsu by the College of Engineering and Science at Purdue University Northwest (PNW) and financial support for the research by PNW.
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Barbara Szonyi is an independent consultant.
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Gizachew, D., Hsu, YC., Szonyi, B. et al. Effect of water activity, temperature, and incubation period on fungal growth and ochratoxin A production on Nyjer seeds. Mycotoxin Res 35, 1–8 (2019). https://doi.org/10.1007/s12550-018-0325-2
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DOI: https://doi.org/10.1007/s12550-018-0325-2