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Natural Occurrence of Mycotoxins in Staple Cereals from Ethiopia

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Abstract

The occurrence of mycotoxins in barley, sorghum, teff (Eragrostis tef) and wheat from Ethiopia has been studied. Samples were analyzed for aflatoxin B1 (AFB1), ochratoxin A (OTA), deoxynivalenol (DON), nivalenol (NIV) and zearalenone (ZEN) using high performance liquid chromatography (HPLC) and for fumonisins (FUM) using enzyme linked immunosorbent assay (ELISA). AFB1 and OTA were detected in samples of all the four crops. AFB1 was detected in 8.8% of the 352 samples analyzed at concentrations ranging from trace to 26 μg kg−1. OTA occurred in 24.3% of 321 samples at a mean concentration of 54.1 μg kg−1 and a maximum of 2106 μg kg−1. DON occurred in barley, sorghum and wheat at 40–2340 μg kg−1 with an overall incidence of 48.8% among the 84 mainly ‘suspect’ samples analyzed; NIV was co-analyzed with DON and was detected at 40 μg kg−1 in a wheat sample and at 50, 380, and 490 μg kg−1 in three sorghum samples. FUM and ZEN occurred only in sorghum samples with low frequencies at concentrations reaching 2117 and 32 μg kg−1, respectively. The analytical results indicate higher mycotoxin contamination in sorghum, which could be related to the widespread storage of sorghum grain in underground pits leading to elevated seed moisture contents. This is the first report on the occurrence of OTA in teff.

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Abbreviations

AFB1:

Aflatoxin B1

DON:

Deoxynivalenol

FUM:

Fumonisins

NIV:

Nivalenol

OTA:

Ochratoxin A

ZEN:

Zearalenone

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Authors and Affiliations

  1. Department of Plant Sciences, Alemaya University, P.O. Box 241, Alemaya, Ethiopia

    Amare Ayalew

  2. Institut für Planzenpathologie und Pflanzenschutz, Universität Göttingen, Grisebachstraße 6, 37077, Göttingen, Germany

    Hartmut Fehrmann

  3. Bayerische Landesanstalt für Landwirtschaft, Lange Point 6, 85354, Freising, Germany

    Johann Lepschy & Robert Beck

  4. Department of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

    Dawit Abate

Authors
  1. Amare Ayalew
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  2. Hartmut Fehrmann
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  3. Johann Lepschy
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  4. Robert Beck
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  5. Dawit Abate
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Correspondence to Amare Ayalew.

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Ayalew, A., Fehrmann, H., Lepschy, J. et al. Natural Occurrence of Mycotoxins in Staple Cereals from Ethiopia. Mycopathologia 162, 57–63 (2006). https://doi.org/10.1007/s11046-006-0027-8

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  • DOI: https://doi.org/10.1007/s11046-006-0027-8

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